Flightless I (FLII) (NM_001256264) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLI; Fli1; FLIL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332326 representing NM_001256264. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACCTCCGAGGCCTCCGCCCAGTGCCTGGCGGCTACTTCCCTGAGAATGTCAAGGCCATGACCAGC CTGCGGTGGCTGAAGCTGAACCGCACTGGCCTCTGCTACCTGCCCGAGGAGCTGGCCGCCCTGCAGAAG CTGGAACACTTGTCTGTGAGCCACAACAACCTGACCACGCTTCATGGGGAGCTGTCCAGCCTGCCATCG CTGCGCGCCATCGTGGCCCGAGCCAACAGTCTGAAGAATTCCGGAGTCCCCGATGACATCTTCAAGCTA GATGATCTCTCAGTCCTGGACTTGAGCCACAACCAGCTGACAGAGTGCCCGCGGGAGCTGGAGAACGCC AAGAACATGCTGGTGCTGAACCTCAGCCACAACAGCATCGACACCATCCCCAACCAGCTCTTCATCAAC CTCACTGACCTACTATACCTGGACCTCAGCGAGAACCGCCTGGAGAGCCTGCCCCCGCAGATGCGCCGC CTGGTGCACCTGCAGACGCTCGTGCTCAATGGAAACCCCCTGCTGCATGCACAGCTCCGGCAGCTCCCA GCGATGACGGCCCTGCAGACCCTGCACCTGCGGAGCACCCAGCGCACCCAGAGCAACCTGCCCACCAGC CTGGAGGGTCTGAGCAACCTCGCAGACGTGGATCTGTCCTGCAATGACCTGACACGGGTGCCCGAGTGT CTGTACACCCTCCCCAGCCTGCGCCGCCTCAACCTCAGCAGCAACCAGATCACGGAGCTGTCCCTGTGC ATAGACCAGTGGGTGCACGTGGAAACTCTGAACCTGTCCCGAAATCAGCTCACCTCACTGCCCTCAGCC ATTTGCAAGCTGAGCAAGCTGAAGAAGCTGTACCTGAATTCCAACAAGCTGGACTTTGACGGGCTGCCC TCAGGCATTGGCAAGCTCACCAACCTGGAAGAGTTCATGGCTGCCAACAACAACCTGGAGCTGGTCCCT GAAAGTCTCTGCAGGTGCCCAAAGCTGAGGAAACTTGTCCTGAACAAGAACCACCTGGTGACCCTCCCA GAAGCCATCCATTTCCTGACGGAGATCGAGGTCCTGGATGTGCGGGAGAACCCCAACCTGGTCATGCCG CCCAAGCCCGCAGACCGTGCCGCTGAGTGGTACAACATCGACTTCTCGCTGCAGAACCAGCTGCGGCTA GCGGGTGCCTCTCCTGCTACCGTGGCTGCAGCTGCAGCTGCAGGGAGTGGGCCCAAGGACCCTATGGCT CGCAAGATGCGACTGCGGAGGCGCAAGGATTCAGCCCAGGATGACCAGGCCAAGCAGGTGCTGAAGGGC ATGTCAGATGTTGCCCAGGAGAAGAACAAAAAGCAGGAGGAGAGCGCAGATGCCCGGGCCCCCAGCGGG AAGGTGCGGCGTTGGGACCAGGGCCTGGAGAAGCCCCGCCTTGACTACTCCGAGTTCTTCACGGAGGAC GTGGGCCAGCTGCCCGGACTGACCATCTGGCAGATAGAGAACTTCGTGCCTGTGCTGGTGGAGGAAGCC TTCCACGGCAAGTTCTACGAGGCTGACTGCTACATTGTGCTCAAGACCTTTCTGGATGACAGCGGCTCC CTCAACTGGGAGATCTACTACTGGATTGGCGGGGAGGCCACACTCGACAAGAAAGCTTGCTCTGCCATC CACGCTGTCAACTTGCGCAACTACCTGGGTGCTGAGTGCCGCACTGTCCGGGAGGAGATGGGCGATGAG AGCGAGGAGTTCCTGCAGGTGTTTGACAACGACATCTCCTACATTGAGGGTGGAACAGCCAGTGGCTTC TACACTGTGGAAGACACACACTATGTCACCAGGATGTATCGTGTGTATGGGAAAAAGAACATCAAGTTG GAGCCTGTGCCCCTCAAGGGGACCTCTCTGGACCCAAGGTTTGTTTTCCTGCTGGACCGAGGGCTAGAC ATCTACGTATGGCGGGGGGCCCAGGCCACACTGAGCAGCACCACCAAGGCCAGGCTCTTTGCAGAGAAA ATTAACAAGAATGAGCGGAAAGGGAAGGCTGAGATCACACTGCTGGTGCAGGGCCAGGAGCTCCCAGAG TTCTGGGAGGCACTGGGTGGGGAGCCCTCTGAGATCAAGAAGCACGTGCCTGAAGACTTCTGGCCGCCG CAGCCCAAGCTGTACAAGGTGGGCCTGGGCTTGGGCTACCTGGAGCTGCCACAGATCAACTACAAGCTC TCCGTGGAACATAAGCAGCGTCCCAAGGTGGAGCTGATGCCAAGAATGCGGCTGCTGCAGAGTCTGCTG GACACGCGCTGCGTGTACATTCTGGACTGTTGGTCCGACGTGTTCATCTGGCTCGGCCGCAAGTCCCCG CGCCTGGTGCGCGCTGCCGCCCTCAAGCTGGGTCAGGAGCTGTGCGGGATGCTGCACCGGCCACGCCAT GCCACGGTCAGCCGCAGCCTCGAGGGCACCGAGGCGCAGGTGTTCAAGGCCAAGTTCAAGAATTGGGAC GATGTGTTGACGGTGGACTACACACGCAATGCGGAGGCCGTGCTGCAGAGCCCGGGTCTCTCCGGGAAG GTGAAACGCGACGCCGAGAAGAAAGACCAGATGAAGGCTGACCTCACTGCGCTTTTCCTGCCGCGGCAG CCGCCCATGTCGCTGGCCGAGGCGGAGCAGCTGATGGAGGAGTGGAACGAAGACCTAGACGGCATGGAG GGTTTCGTGCTGGAGGGCAAGAAGTTTGCGCGGCTGCCGGAAGAGGAGTTTGGCCACTTCTACACGCAG GACTGCTACGTCTTCCTCTGCAGGTACTGGGTGCCTGTGGAGTACGAGGAGGAGGAAAAGAAGGAAGAC AAGGAGGAGAAGGCCGAGGGCAAAGAAGGCGAGGAAGCAACCGCTGAGGCAGAGGAGAAGCAGCCAGAG GAGGACTTCCAGTGCATCGTGTACTTCTGGCAGGGCCGTGAAGCCTCCAATATGGGCTGGCTCACCTTC ACCTTCAGCCTGCAAAAGAAGTTCGAGAGCCTCTTCCCTGGGAAGCTGGAGGTGGTACGCATGACGCAG CAGCAGGAGAACCCCAAGTTCCTGTCCCATTTCAAGAGGAAGTTCATCATCCACCGGGGCAAGAGGAAG GCGGTCCAGGGCGCCCAACAGCCCAGCCTCTACCAGATCCGCACCAACGGCAGCGCCCTCTGCACCCGG TGCATCCAGATCAACACCGACTCCAGCCTCCTCAACTCCGAGTTCTGCTTCATCCTCAAGGTTCCCTTT GAGAGTGAGGACAACCAGGGCATCGTGTATGCCTGGGTGGGCCGGGCATCAGACCCTGACGAAGCCAAG TTGGCAGAAGACATCCTGAACACCATGTTTGACACCTCCTACAGCAAGCAGGTTATCAACGAAGGTGAG GAGCCTGAGAACTTCTTCTGGGTGGGCATTGGGGCACAGAAGCCCTATGATGACGATGCCGAGTACATG AAACACACACGTCTCTTCCGGTGCTCCAACGAGAAGGGCTACTTTGCAGTGACTGAGAAATGCTCCGAC TTTTGCCAAGATGACCTGGCAGATGATGACATCATGTTGCTAGACAATGGCCAAGAGGTCTACATGTGG GTGGGGACCCAGACTAGCCAGGTGGAGATCAAGCTGAGCCTGAAGGCCTGCCAGGTATATATCCAGCAC ATGCGGTCCAAGGAACATGAGCGGCCGCGCCGGCTGCGCCTGGTCCGCAAGGGCAATGAGCAGCACGCC TTTACCCGCTGCTTCCACGCCTGGAGCGCCTTCTGCAAGGCCCTGGCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256264
Insert Size	3777 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256264.1
RefSeq Size	4208 bp
RefSeq ORF	3777 bp
Locus ID	2314
UniProt ID	Q13045
Protein Families	ES Cell Differentiation/IPS, Transcription Factors
MW	143.7 kDa
Gene Summary	This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) has an alternate 5' exon, which includes the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.

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