Girdin (CCDC88A) (NM_001254943) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APE; GIRDIN; GIV; GRDN; HkRP1; KIAA1212; PEHO; PEHOL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332271 representing NM_001254943. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGAACGAAATTTTTACTCCCCTTCTGGAGCAGTTCATGACCAGCCCTTTGGTCACTTGGGTTAAA ACGTTTGGACCTCTGGCCGCAGGAAATGGGACCAACCTTGATGAATATGTGGCTTTGGTGGATGGGGTA TTCTTGAACCAGGTCATGCTCCAAATTAATCCTAAATTGGAGAGTCAGAGAGTAAATAAAAAAGTCAAT AATGATGCCTCACTTAGAATGCACAATCTATCCATTTTGGTGAGACAGATAAAATTTTATTACCAGGAG ACTTTGCAGCAATTGATCATGATGTCGTTGCCAAATGTCTTAATCATTGGCAAAAATCCCTTTTCTGAA CAAGGCACAGAAGAAGTTAAAAAACTGCTTTTACTTTTATTGGGTTGTGCAGTTCAGTGTCAGAAAAAA GAGGAATTTATTGAAAGAATTCAAGGTTTAGATTTTGATACAAAAGCAGCGGTTGCCGCACATATTCAA GAGGTAACTCATAATCAGGAAAATGTGTTTGACCTGCAATGGATGGAAGTGACTGATATGTCGCAGGAG GACATAGAACCACTCTTGAAAAATATGGCATTGCATCTAAAAAGACTTATAGATGAGAGAGATGAACAT TCAGAGACTATCATAGAACTCTCTGAAGAGCGGGATGGTCTCCATTTTCTACCCCATGCCTCTTCATCT GCACAGTCACCCTGTGGTTCTCCAGGCATGAAGCGAACAGAAAGTCGACAACATCTGTCGGTGGAACTG GCAGATGCTAAAGCCAAGATAAGAAGGCTTAGGCAGGAATTGGAGGAAAAGACTGAGCAGTTGTTGGAT TGTAAACAAGAACTTGAGCAAATGGAAATAGAACTCAAAAGGCTGCAACAAGAGAACATGAATTTGCTT TCGGATGCTCGCTCTGCCAGAATGTACCGAGATGAATTAGATGCACTTCGAGAGAAAGCAGTCAGAGTC GATAAGCTTGAAAGTGAAGTCAGCAGATATAAAGAGAGACTACATGATATTGAATTTTATAAGGCAAGA GTTGAGGAATTAAAAGAAGACAATCAAGTTTTATTAGAAACAAAAACCATGTTGGAAGACCAACTAGAG GGAACTCGTGCTCGTTCTGATAAATTACATGAATTAGAAAAAGAGAACTTACAACTGAAAGCTAAACTT CATGATATGGAAATGGAACGAGATATGGATAGAAAAAAGATTGAAGAATTAATGGAAGAAAATATGACT TTGGAAATGGCACAGAAACAAAGTATGGATGAATCATTACATCTTGGCTGGGAACTGGAACAGATATCC AGAACTAGTGAACTTTCCGAAGCACCCCAGAAATCCCTGGGCCATGAGGTGAATGAGTTGACATCAAGT AGATTATTGAAGCTAGAGATGGAAAATCAAAGTTTGACAAAAACCGTAGAAGAGCTTCGGACTACTGTG GATTCTGTAGAAGGCAATGCTTCCAAAATCCTGAAAATGGAAAAAGAAAATCAAAGGCTCAGTAAAAAG GTTGAGATTCTTGAAAATGAGATTGTTCAAGAAAAGCAAAGTCTTCAGAATTGTCAGAATTTAAGCAAG GATCTAATGAAGGAGAAAGCTCAGCTTGAAAAAACAATAGAAACACTGAGAGAAAATTCAGAGAGACAG ATTAAGATACTGGAACAGGAAAATGAACATCTGAATCAAACAGTGTCTTCCTTAAGGCAGCGGTCCCAG ATAAGTGCAGAAGCAAGAGTGAAAGACATTGAAAAAGAAAACAAAATTCTTCATGAATCTATCAAAGAA ACAAGTAGCAAGCTAAGCAAGATTGAATTTGAAAAAAGACAAATTAAAAAAGAATTGGAACATTATAAA GAAAAAGGAGAACGAGCTGAAGAACTTGAAAATGAATTGCATCATCTTGAAAAAGAAAATGAATTATTA CAGAAAAAAATAACTAATTTAAAAATTACTTGTGAAAAAATTGAGGCCTTAGAACAAGAAAATTCAGAG CTAGAAAGAGAAAATAGAAAATTAAAAAAAACATTGGATAGCTTTAAAAATCTGACCTTTCAGTTAGAA TCCCTAGAAAAAGAGAATTCCCAACTTGATGAGGAAAACTTAGAACTGCGAAGGAATGTAGAATCTTTG AAGTGTGCAAGCATGAAAATGGCTCAGCTACAGCTAGAAAACAAAGAACTGGAAAGTGAAAAAGAGCAA CTTAAGAAGGGTTTGGAGCTCCTGAAAGCATCTTTCAAGAAAACAGAACGCTTAGAAGTTAGCTACCAG GGTTTAGATATAGAAAATCAAAGACTGCAAAAAACTTTAGAGAACAGCAATAAAAAAATCCAGCAATTA GAGAGTGAACTACAAGACTTAGAGATGGAAAATCAAACATTGCAGAAAAACCTAGAAGAACTAAAAATA TCTAGCAAAAGACTAGAACAGCTGGAAAAAGAAAATAAATCATTAGAGCAAGAGACTTCTCAACTGGAA AAGGATAAGAAACAATTGGAGAAGGAAAATAAGAGACTCCGACAACAAGCAGAAATTAAAGATACCACA TTAGAAGAAAATAATGTGAAGATTGGAAATTTGGAAAAAGAAAACAAAACCCTATCCAAAGAAATTGGT ATATATAAAGAATCTTGTGTCCGTCTGAAAGAACTAGAAAAAGAAAATAAGGAGCTTGTGAAAAGAGCA ACTATTGATATAAAAACGTTGGTTACACTACGTGAGGATTTGGTGAGTGAAAAGTTGAAGACCCAACAG ATGAACAATGATCTCGAAAAATTAACTCATGAGCTTGAGAAGATAGGGTTAAATAAGGAGCGACTCTTA CATGATGAACAAAGTACTGATGACAGGTATAAACTTTTGGAATCAAAATTAGAATCCACTCTTAAGAAG TCTCTTGAAATAAAAGAAGAAAAAATTGCTGCTTTAGAAGCTCGATTAGAAGAATCCACGAATTATAAC CAGCAATTGCGCCAAGAACTTAAAACAGTGAAAAAAAATTATGAAGCTCTCAAACAGAGACAAGATGAG GAAAGGATGGTACAGAGCTCTCCTCCAATATCTGGTGAAGACAACAAATGGGAGCGAGAAAGTCAAGAA ACGACTAGAGAACTTCTGAAAGTTAAAGACAGATTAATTGAAGTAGAAAGAAATAATGCTACACTGCAA GCAGAGAAGCAAGCGTTGAAAACTCAACTGAAGCAACTTGAGACACAGAACAATAATTTGCAGGCTCAG ATTCTTGCACTTCAGAGGCAGACAGTGTCATTACAAGAACAGAATACCACTCTTCAAACACAGAATGCC AAGCTTCAGGTTGAAAATTCCACCCTTAATTCCCAAAGTACCTCACTCATGAACCAGAATGCCCAACTC CTAATCCAGCAGTCTTCCTTAGAAAATGAAAATGAATCTGTAATCAAAGAGCGAGAAGACCTAAAATCT CTCTATGATTCTCTGATCAAAGATCATGAAAAGCTGGAACTTCTTCATGAACGTCAGGCTTCAGAGTAT GAATCTCTTATCTCTAAACATGGAACTCTGAAGTCTGCCCACAAAAATCTTGAGGTGGAACATAGAGAC CTTGAAGACCGTTACAATCAGTTATTAAAACAGAAAGGACAGTTGGAAGATTTGGAAAAAATGCTCAAA GTAGAACAGGAAAAAATGCTGCTTGAAAATAAAAATCATGAAACAGTAGCTGCAGAATACAAGAAACTT TGTGGTGAAAATGATAGGCTGAATCATACCTATAGTCAACTTTTAAAAGAGACTGAAGTTTTACAAACT GACCATAAAAATTTGAAAAGTCTTCTGAATAATTCCAAACTGGAACAAACAAGATTAGAAGCTGAATTT TCAAAACTAAAGGAACAATACCAACAATTGGATATTACATCAACCAAGCTGAATAACCAGTGTGAGTTG CTAAGCCAACTTAAAGGAAATTTAGAAGAAGAAAATCGGCATCTACTAGATCAAATTCAGACATTAATG CTACAGAACAGAACACTTTTGGAGCAGAATATGGAAAGCAAGGATCTTTTTCATGTTGAACAAAGACAG TACATTGATAAGTTAAATGAATTAAGACGTCAGAAGGAGAAACTAGAAGAGAAAATTATGGATCAATAC AAATTTTATGACCCATCTCCTCCTAGAAGGAGAGGCAACTGGATTACTCTAAAAATGAGAAAATTGATA AAGTCTAAGAAAGATATTAATCGGGAACGCCAGAAATCTCTAACATTAACACCCACCCGCTCAGACTCC AGTGAAGGATTTCTTCAGCTCCCTCATCAAGACAGTCAAGATAGTTCTTCAGTAGGTTCAAACTCTTTA GAAGATGGCCAGACCTTGGGGACCAAGAAAAGCAGCATGGTTGCACTGAAAAGACTGCCCTTTTTGAGG AACAGACCGAAGGATAAAGACAAAATGAAGGCCTGCTACCGTCGTTCCATGTCCATGAATGACCTGGTG CAGTCCATGGTCCTAGCAGGACAGTGGACAGGTAGTACTGAGAATTTGGAGGTTCCTGATGATATTTCA ACGGGTAAAAGGAGAAAAGAATTGGGAGCTATGGCCTTCTCTACTACAGCCATCAACTTTTCAACTGTC AACTCTTCTGCAGGCTTCAGATCCAAGCAGTTGGTTAATAATAAAGATACTACATCCTTTGAAGACATA AGTCCACAAGGTGTTAGTGATGATTCTAGTACGGGATCAAGAGTTCATGCTTCAAGACCAGCCAGCCTT GATAGTGGCAGAACATCCACTAGCAATAGCAATAATAATGCTTCACTACATGAAGTCAAAGCAGGTGCA GTTAATAACCAAAGCAGGCCACAAAGCCACAGCAGTGGAGAATTTAGCCTGCTTCATGACCATGAGGCT TGGTCCAGCAGTGGTAGCAGTCCAATCCAGTACTTGAAAAGACAGACCAGATCAAGCCCAGTGCTCCAG CACAAAATATCTGAAACACTGGAGAGTCGACATCACAAGATCAAAACTGGTTCCCCTGGAAGTGAAGTT GTTACTCTACAACAGTTTTTGGAAGAAAGCAATAAGCTTACCTCAGTACAGATAAAGTCCTCAAGTCAA GAGAATCTTTTAGATGAAGTAATGAAAAGTTTGTCTGTCTCTTCTGACTTTTTGGGAAAAGACAAACCA GTTAGCTGTGGTCTGGCCAGCGTGATCTCAACTGCCGAAGGAACTACACGAAGGACAAGCATCCATGAT TTTTTGACCAAGGACAGTAGACTGCCTATATCAGTTGATTCACCACCAGCTGCTGCTGACAGCAACACC ACTGCAGCATCTAATGTGGACAAAGTACAAGAAAGCAGAAATTCAAAAAGCAGGTCTAGGGAGCAACAA AGCTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001254943
Insert Size	5391 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001254943.1
RefSeq Size	9605 bp
RefSeq ORF	5391 bp
Locus ID	55704
UniProt ID	Q3V6T2
MW	207.8 kDa
Gene Summary	This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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