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Girdin (CCDC88A) (NM_001254943) Human Tagged ORF Clone
CAT＃: RG235337
- TrueORF®
CCDC88A (tGFP-tagged) - Homo sapiens coiled-coil domain containing 88A (CCDC88A), transcript variant 3
ORF Plasmid: DDK
"NM_001254943" in other vectors (2)
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CNY 19,760.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | APE; GIRDIN; GIV; GRDN; HkRP1; KIAA1212; PEHO; PEHOL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235337 representing NM_001254943
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACGAAATTTTTACTCCCCTTCTGGAGCAGTTCATGACCAGCCCTTTGGTCACTTGGGTTAAAA CGTTTGGACCTCTGGCCGCAGGAAATGGGACCAACCTTGATGAATATGTGGCTTTGGTGGATGGGGTATT CTTGAACCAGGTCATGCTCCAAATTAATCCTAAATTGGAGAGTCAGAGAGTAAATAAAAAAGTCAATAAT GATGCCTCACTTAGAATGCACAATCTATCCATTTTGGTGAGACAGATAAAATTTTATTACCAGGAGACTT TGCAGCAATTGATCATGATGTCGTTGCCAAATGTCTTAATCATTGGCAAAAATCCCTTTTCTGAACAAGG CACAGAAGAAGTTAAAAAACTGCTTTTACTTTTATTGGGTTGTGCAGTTCAGTGTCAGAAAAAAGAGGAA TTTATTGAAAGAATTCAAGGTTTAGATTTTGATACAAAAGCAGCGGTTGCCGCACATATTCAAGAGGTAA CTCATAATCAGGAAAATGTGTTTGACCTGCAATGGATGGAAGTGACTGATATGTCGCAGGAGGACATAGA ACCACTCTTGAAAAATATGGCATTGCATCTAAAAAGACTTATAGATGAGAGAGATGAACATTCAGAGACT ATCATAGAACTCTCTGAAGAGCGGGATGGTCTCCATTTTCTACCCCATGCCTCTTCATCTGCACAGTCAC CCTGTGGTTCTCCAGGCATGAAGCGAACAGAAAGTCGACAACATCTGTCGGTGGAACTGGCAGATGCTAA AGCCAAGATAAGAAGGCTTAGGCAGGAATTGGAGGAAAAGACTGAGCAGTTGTTGGATTGTAAACAAGAA CTTGAGCAAATGGAAATAGAACTCAAAAGGCTGCAACAAGAGAACATGAATTTGCTTTCGGATGCTCGCT CTGCCAGAATGTACCGAGATGAATTAGATGCACTTCGAGAGAAAGCAGTCAGAGTCGATAAGCTTGAAAG TGAAGTCAGCAGATATAAAGAGAGACTACATGATATTGAATTTTATAAGGCAAGAGTTGAGGAATTAAAA GAAGACAATCAAGTTTTATTAGAAACAAAAACCATGTTGGAAGACCAACTAGAGGGAACTCGTGCTCGTT CTGATAAATTACATGAATTAGAAAAAGAGAACTTACAACTGAAAGCTAAACTTCATGATATGGAAATGGA ACGAGATATGGATAGAAAAAAGATTGAAGAATTAATGGAAGAAAATATGACTTTGGAAATGGCACAGAAA CAAAGTATGGATGAATCATTACATCTTGGCTGGGAACTGGAACAGATATCCAGAACTAGTGAACTTTCCG AAGCACCCCAGAAATCCCTGGGCCATGAGGTGAATGAGTTGACATCAAGTAGATTATTGAAGCTAGAGAT GGAAAATCAAAGTTTGACAAAAACCGTAGAAGAGCTTCGGACTACTGTGGATTCTGTAGAAGGCAATGCT TCCAAAATCCTGAAAATGGAAAAAGAAAATCAAAGGCTCAGTAAAAAGGTTGAGATTCTTGAAAATGAGA TTGTTCAAGAAAAGCAAAGTCTTCAGAATTGTCAGAATTTAAGCAAGGATCTAATGAAGGAGAAAGCTCA GCTTGAAAAAACAATAGAAACACTGAGAGAAAATTCAGAGAGACAGATTAAGATACTGGAACAGGAAAAT GAACATCTGAATCAAACAGTGTCTTCCTTAAGGCAGCGGTCCCAGATAAGTGCAGAAGCAAGAGTGAAAG ACATTGAAAAAGAAAACAAAATTCTTCATGAATCTATCAAAGAAACAAGTAGCAAGCTAAGCAAGATTGA ATTTGAAAAAAGACAAATTAAAAAAGAATTGGAACATTATAAAGAAAAAGGAGAACGAGCTGAAGAACTT GAAAATGAATTGCATCATCTTGAAAAAGAAAATGAATTATTACAGAAAAAAATAACTAATTTAAAAATTA CTTGTGAAAAAATTGAGGCCTTAGAACAAGAAAATTCAGAGCTAGAAAGAGAAAATAGAAAATTAAAAAA AACATTGGATAGCTTTAAAAATCTGACCTTTCAGTTAGAATCCCTAGAAAAAGAGAATTCCCAACTTGAT GAGGAAAACTTAGAACTGCGAAGGAATGTAGAATCTTTGAAGTGTGCAAGCATGAAAATGGCTCAGCTAC AGCTAGAAAACAAAGAACTGGAAAGTGAAAAAGAGCAACTTAAGAAGGGTTTGGAGCTCCTGAAAGCATC TTTCAAGAAAACAGAACGCTTAGAAGTTAGCTACCAGGGTTTAGATATAGAAAATCAAAGACTGCAAAAA ACTTTAGAGAACAGCAATAAAAAAATCCAGCAATTAGAGAGTGAACTACAAGACTTAGAGATGGAAAATC AAACATTGCAGAAAAACCTAGAAGAACTAAAAATATCTAGCAAAAGACTAGAACAGCTGGAAAAAGAAAA TAAATCATTAGAGCAAGAGACTTCTCAACTGGAAAAGGATAAGAAACAATTGGAGAAGGAAAATAAGAGA CTCCGACAACAAGCAGAAATTAAAGATACCACATTAGAAGAAAATAATGTGAAGATTGGAAATTTGGAAA AAGAAAACAAAACCCTATCCAAAGAAATTGGTATATATAAAGAATCTTGTGTCCGTCTGAAAGAACTAGA AAAAGAAAATAAGGAGCTTGTGAAAAGAGCAACTATTGATATAAAAACGTTGGTTACACTACGTGAGGAT TTGGTGAGTGAAAAGTTGAAGACCCAACAGATGAACAATGATCTCGAAAAATTAACTCATGAGCTTGAGA AGATAGGGTTAAATAAGGAGCGACTCTTACATGATGAACAAAGTACTGATGACAGGTATAAACTTTTGGA ATCAAAATTAGAATCCACTCTTAAGAAGTCTCTTGAAATAAAAGAAGAAAAAATTGCTGCTTTAGAAGCT CGATTAGAAGAATCCACGAATTATAACCAGCAATTGCGCCAAGAACTTAAAACAGTGAAAAAAAATTATG AAGCTCTCAAACAGAGACAAGATGAGGAAAGGATGGTACAGAGCTCTCCTCCAATATCTGGTGAAGACAA CAAATGGGAGCGAGAAAGTCAAGAAACGACTAGAGAACTTCTGAAAGTTAAAGACAGATTAATTGAAGTA GAAAGAAATAATGCTACACTGCAAGCAGAGAAGCAAGCGTTGAAAACTCAACTGAAGCAACTTGAGACAC AGAACAATAATTTGCAGGCTCAGATTCTTGCACTTCAGAGGCAGACAGTGTCATTACAAGAACAGAATAC CACTCTTCAAACACAGAATGCCAAGCTTCAGGTTGAAAATTCCACCCTTAATTCCCAAAGTACCTCACTC ATGAACCAGAATGCCCAACTCCTAATCCAGCAGTCTTCCTTAGAAAATGAAAATGAATCTGTAATCAAAG AGCGAGAAGACCTAAAATCTCTCTATGATTCTCTGATCAAAGATCATGAAAAGCTGGAACTTCTTCATGA ACGTCAGGCTTCAGAGTATGAATCTCTTATCTCTAAACATGGAACTCTGAAGTCTGCCCACAAAAATCTT GAGGTGGAACATAGAGACCTTGAAGACCGTTACAATCAGTTATTAAAACAGAAAGGACAGTTGGAAGATT TGGAAAAAATGCTCAAAGTAGAACAGGAAAAAATGCTGCTTGAAAATAAAAATCATGAAACAGTAGCTGC AGAATACAAGAAACTTTGTGGTGAAAATGATAGGCTGAATCATACCTATAGTCAACTTTTAAAAGAGACT GAAGTTTTACAAACTGACCATAAAAATTTGAAAAGTCTTCTGAATAATTCCAAACTGGAACAAACAAGAT TAGAAGCTGAATTTTCAAAACTAAAGGAACAATACCAACAATTGGATATTACATCAACCAAGCTGAATAA CCAGTGTGAGTTGCTAAGCCAACTTAAAGGAAATTTAGAAGAAGAAAATCGGCATCTACTAGATCAAATT CAGACATTAATGCTACAGAACAGAACACTTTTGGAGCAGAATATGGAAAGCAAGGATCTTTTTCATGTTG AACAAAGACAGTACATTGATAAGTTAAATGAATTAAGACGTCAGAAGGAGAAACTAGAAGAGAAAATTAT GGATCAATACAAATTTTATGACCCATCTCCTCCTAGAAGGAGAGGCAACTGGATTACTCTAAAAATGAGA AAATTGATAAAGTCTAAGAAAGATATTAATCGGGAACGCCAGAAATCTCTAACATTAACACCCACCCGCT CAGACTCCAGTGAAGGATTTCTTCAGCTCCCTCATCAAGACAGTCAAGATAGTTCTTCAGTAGGTTCAAA CTCTTTAGAAGATGGCCAGACCTTGGGGACCAAGAAAAGCAGCATGGTTGCACTGAAAAGACTGCCCTTT TTGAGGAACAGACCGAAGGATAAAGACAAAATGAAGGCCTGCTACCGTCGTTCCATGTCCATGAATGACC TGGTGCAGTCCATGGTCCTAGCAGGACAGTGGACAGGTAGTACTGAGAATTTGGAGGTTCCTGATGATAT TTCAACGGGTAAAAGGAGAAAAGAATTGGGAGCTATGGCCTTCTCTACTACAGCCATCAACTTTTCAACT GTCAACTCTTCTGCAGGCTTCAGATCCAAGCAGTTGGTTAATAATAAAGATACTACATCCTTTGAAGACA TAAGTCCACAAGGTGTTAGTGATGATTCTAGTACGGGATCAAGAGTTCATGCTTCAAGACCAGCCAGCCT TGATAGTGGCAGAACATCCACTAGCAATAGCAATAATAATGCTTCACTACATGAAGTCAAAGCAGGTGCA GTTAATAACCAAAGCAGGCCACAAAGCCACAGCAGTGGAGAATTTAGCCTGCTTCATGACCATGAGGCTT GGTCCAGCAGTGGTAGCAGTCCAATCCAGTACTTGAAAAGACAGACCAGATCAAGCCCAGTGCTCCAGCA CAAAATATCTGAAACACTGGAGAGTCGACATCACAAGATCAAAACTGGTTCCCCTGGAAGTGAAGTTGTT ACTCTACAACAGTTTTTGGAAGAAAGCAATAAGCTTACCTCAGTACAGATAAAGTCCTCAAGTCAAGAGA ATCTTTTAGATGAAGTAATGAAAAGTTTGTCTGTCTCTTCTGACTTTTTGGGAAAAGACAAACCAGTTAG CTGTGGTCTGGCCAGCGTGATCTCAACTGCCGAAGGAACTACACGAAGGACAAGCATCCATGATTTTTTG ACCAAGGACAGTAGACTGCCTATATCAGTTGATTCACCACCAGCTGCTGCTGACAGCAACACCACTGCAG CATCTAATGTGGACAAAGTACAAGAAAGCAGAAATTCAAAAAGCAGGTCTAGGGAGCAACAAAGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235337 representing NM_001254943
Red=Cloning site Green=Tags(s) MENEIFTPLLEQFMTSPLVTWVKTFGPLAAGNGTNLDEYVALVDGVFLNQVMLQINPKLESQRVNKKVNN DASLRMHNLSILVRQIKFYYQETLQQLIMMSLPNVLIIGKNPFSEQGTEEVKKLLLLLLGCAVQCQKKEE FIERIQGLDFDTKAAVAAHIQEVTHNQENVFDLQWMEVTDMSQEDIEPLLKNMALHLKRLIDERDEHSET IIELSEERDGLHFLPHASSSAQSPCGSPGMKRTESRQHLSVELADAKAKIRRLRQELEEKTEQLLDCKQE LEQMEIELKRLQQENMNLLSDARSARMYRDELDALREKAVRVDKLESEVSRYKERLHDIEFYKARVEELK EDNQVLLETKTMLEDQLEGTRARSDKLHELEKENLQLKAKLHDMEMERDMDRKKIEELMEENMTLEMAQK QSMDESLHLGWELEQISRTSELSEAPQKSLGHEVNELTSSRLLKLEMENQSLTKTVEELRTTVDSVEGNA SKILKMEKENQRLSKKVEILENEIVQEKQSLQNCQNLSKDLMKEKAQLEKTIETLRENSERQIKILEQEN EHLNQTVSSLRQRSQISAEARVKDIEKENKILHESIKETSSKLSKIEFEKRQIKKELEHYKEKGERAEEL ENELHHLEKENELLQKKITNLKITCEKIEALEQENSELERENRKLKKTLDSFKNLTFQLESLEKENSQLD EENLELRRNVESLKCASMKMAQLQLENKELESEKEQLKKGLELLKASFKKTERLEVSYQGLDIENQRLQK TLENSNKKIQQLESELQDLEMENQTLQKNLEELKISSKRLEQLEKENKSLEQETSQLEKDKKQLEKENKR LRQQAEIKDTTLEENNVKIGNLEKENKTLSKEIGIYKESCVRLKELEKENKELVKRATIDIKTLVTLRED LVSEKLKTQQMNNDLEKLTHELEKIGLNKERLLHDEQSTDDRYKLLESKLESTLKKSLEIKEEKIAALEA RLEESTNYNQQLRQELKTVKKNYEALKQRQDEERMVQSSPPISGEDNKWERESQETTRELLKVKDRLIEV ERNNATLQAEKQALKTQLKQLETQNNNLQAQILALQRQTVSLQEQNTTLQTQNAKLQVENSTLNSQSTSL MNQNAQLLIQQSSLENENESVIKEREDLKSLYDSLIKDHEKLELLHERQASEYESLISKHGTLKSAHKNL EVEHRDLEDRYNQLLKQKGQLEDLEKMLKVEQEKMLLENKNHETVAAEYKKLCGENDRLNHTYSQLLKET EVLQTDHKNLKSLLNNSKLEQTRLEAEFSKLKEQYQQLDITSTKLNNQCELLSQLKGNLEEENRHLLDQI QTLMLQNRTLLEQNMESKDLFHVEQRQYIDKLNELRRQKEKLEEKIMDQYKFYDPSPPRRRGNWITLKMR KLIKSKKDINRERQKSLTLTPTRSDSSEGFLQLPHQDSQDSSSVGSNSLEDGQTLGTKKSSMVALKRLPF LRNRPKDKDKMKACYRRSMSMNDLVQSMVLAGQWTGSTENLEVPDDISTGKRRKELGAMAFSTTAINFST VNSSAGFRSKQLVNNKDTTSFEDISPQGVSDDSSTGSRVHASRPASLDSGRTSTSNSNNNASLHEVKAGA VNNQSRPQSHSSGEFSLLHDHEAWSSSGSSPIQYLKRQTRSSPVLQHKISETLESRHHKIKTGSPGSEVV TLQQFLEESNKLTSVQIKSSSQENLLDEVMKSLSVSSDFLGKDKPVSCGLASVISTAEGTTRRTSIHDFL TKDSRLPISVDSPPAAADSNTTAASNVDKVQESRNSKSRSREQQSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001254943 |
| ORF Size | 5388 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001254943.2 |
| RefSeq Size | 9605 bp |
| RefSeq ORF | 5391 bp |
| Locus ID | 55704 |
| UniProt ID | Q3V6T2 |
| Gene Summary | This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
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