AP4B1 (NM_001253852) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BETA-4; CPSQ5; SPG47
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332243 representing NM_001253852. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGTACCTTGGCTCCGAGGACGTGGTGAAGGAGCTGAAGAAGGCTCTGTGCAATCCTCACATTCAA GCTGATAGGCTGCGCTACCGGAATGTCATCCAGCGAGTGATTAGGTACATGACTCAAGGCTTGGACATG TCTGGTGTTTTTATGGAAATGGTGAAGGCCAGTGCCACTGTAGATATTGTCCAGAAGAAGTTGGTTTAT CTGTACATGTGCACATATGCTCCCCTGAAACCAGATCTGGCTCTCCTGGCCATCAATACGCTGTGCAAA GACTGCTCAGACCCCAATCCAATGGTGCGAGGGCTGGCGTTACGGAGCATGTGTAGCCTCAGGATGCCT GGTGTGCAGGAGTATATACAACAGCCTATTCTCAATGGTCTGCGGGATAAGGCTTCATATGTCAGGAGA GTGGCAGTCCTTGGATGTGCCAAGATGCATAATCTTCATGGAGACTCTGAAGTAGATGGTGCCCTGGTA AATGAATTATACAGTTTGCTGCGTGACCAGGATCCAATTGTAGTTGTGAACTGCTTGAGGTCTCTAGAG GAAATTCTGAAACAGGAAGGAGGCGTTGTCATCAATAAGCCCATTGCTCACCATCTCTTAAATCGAATG TCAAAACTGGACCAATGGGGCCAGGCTGAAGTATTGAACTTTCTGCTACGCTACCAACCCCGCAGTGAG GAAGAACTATTTGACATTCTCAATCTGTTGGATAGTTTCCTCAAGAGCAGTAGCCCAGGTGTGGTGATG GGAGCTACCAAACTTTTTCTGATCTTGGCAAAAATGTTTCCCCACGTACAAACTGATGTCCTTGTGCGG GTCAAGGGACCTTTGCTAGCTGCCTGTTCTTCAGAGAGCCGTGAGCTCTGTTTTGTTGCTCTTTGTCAT GTACGCCAGATCTTGCATAGTTTACCAGGTCACTTTAGCAGCCACTACAAAAAGTTTTTTTGCTCCTAC TCGGAGCCCCACTACATCAAACTACAGAAAGTGGAGGTGCTGTGTGAACTGGTGAACGATGAGAATGTG CAGCAGGTGCTAGAGGAGCTTCGAGGGTACTGCACGGATGTGTCTGCGGACTTTGCACAGGCTGCCATC TTTGCCATAGGTGGCATTGCCAGGACTTACACAGATCAATGTGTTCAGATTTTAACAGAGTTGCTGGGT CTTCGACAAGAGCACATTACCACAGTGGTGGTGCAGACTTTCCGAGACCTGGTTTGGTTGTGTCCTCAG TGTACTGAAGCTGTATGTCAGGCCCTGCCCGGCTGTGAAGAGAACATTCAAGATAGTGAGGGGAAGCAA GCACTTATTTGGCTACTTGGTGTCCATGGGGAAAGAATTCCTAATGCTCCTTATGTGTTAGAGGACTTT GTTGAGAATGTGAAGTCGGAAACATTTCCAGCTGTTAAGATGGAGCTGCTCACTGCTTTGCTGCGCCTT TTCCTCTCCCGACCTGCTGAGTGCCAGGACATGCTAGGACGTTTGTTGTATTACTGCATAGAGGAAGAA AAAGATATGGCTGTACGGGACCGAGGTCTCTTCTATTATCGCCTCCTCTTAGTTGGCATTGATGAAGTT AAGCGGATTCTGTGTAGCCCTAAATCTGACCCTACTCTTGGACTTTTGGAGGATCCGGCAGAAAGACCT GTGAATAGCTGGGCCTCAGACTTCAACACACTGGTGCCAGTGTATGGCAAAGCCCACTGGGCAACTATC TCTAAATGCCAGGGGGCAGAGCGTTGTGACCCAGAGCTTCCTAAAACTTCATCCTTTGCCGCATCAGGA CCCTTGATTCCTGAAGAGAACAAGGAGAGGGTACAAGAACTCCCTGATTCTGGAGCCCTCATGCTAGTC CCCAATCGCCAGCTTACTGCTGATTATTTTGAGAAAACTTGGCTTAGCCTTAAAGTTGCTCATCAGCAA GTGTTGCCTTGGCGGGGAGAATTCCATCCTGACACCCTCCAGATGGCTCTTCAAGTAGTGAACATCCAG ACCATCGCAATGAGTAGGGCTGGGTCTCGGCCATGGAAAGCATACCTCAGTGCTCAGGATGATACTGGC TGTCTGTTCTTAACAGAACTGCTATTGGAGCCTGGAAACTCAGAAATGCAGATCTCTGTGAAACAAAAT GAAGCAAGAACGGAGACGCTGAATAGTTTTATTTCTGTATTAGAAACTGTGATTGGAACAATTGAAGAA ATAAAATCATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253852
Insert Size	2220 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253852.1
RefSeq Size	2723 bp
RefSeq ORF	2220 bp
Locus ID	10717
UniProt ID	Q9Y6B7
Protein Pathways	Lysosome
MW	83.3 kDa
Gene Summary	This gene encodes a subunit of a heterotetrameric adapter-like complex 4 that is involved in targeting proteins from the trans-Golgi network to the endosomal-lysosomal system. Mutations in this gene are associated with cerebral palsy spastic quadriplegic type 5 (CPSQ5) disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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