PKD2L1 (NM_001253837) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PCL; PKD2L; PKDL; TRPP3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332239 representing NM_001253837. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAACCCCAGGAGACGGCATACAGGACCCAGGTGTCCAGCTGCTGCCTCCATATCTGTCAAGGCATCA GAGGCATCTCTCCCATGCCCCTTAGGACTTTGGGGAACAACCCTGACTGAGAACACAGCTGAGAACCGG GAACTTTATATCAAGACCACCCTGAGGGAGCTGTTGGTATATATTGTGTTCCTGGTGGACATCTGTCTA CTGACCTATGGAATGACAAGCTCCAGTGCTTATTACTACACCAAAGTGATGTCTGAGCTCTTCTTACAT ACTCCATCAGACACTGGAGTCTCCTTTCAGGCCATCAGCAGCATGGCGGACTTCTGGGATTTTGCCCAG GGCCCACTACTGGACAGTTTGTATTGGACCAAATGGTACAACAACCAGAGCCTGGGCCATGGCTCCCAC TCCTTCATCTACTATGAGAACATGCTGCTGGGGGTTCCGAGGCTGCGGCAGCTAAAGGTCCGCAATGAC TCCTGTGTGGTGCATGAAGACTTCCGGGAGGACATTCTGAGCTGCTATGATGTCTACTCTCCAGACAAA GAAGAACAACTCCCCTTTGGGCCCTTCAATGGCACAGCGTGGACATACCACTCGCAGGATGAGTTGGGG GGCTTCTCCCACTGGGGCAGGCTCACAAGCTACAGCGGAGGTGGCTACTACCTGGACCTTCCAGGATCC CGACAGGGTAGTGCAGAGGCTCTCCGGGCCCTTCAGGAGGGGCTGTGGCTGGACAGGGGCACTCGAGTG GTGTTCATCGACTTCTCAGTCTACAATGCCAATATCAATCTTTTCTGTGTCCTGAGGCTGGTGGTGGAG TTTCCAGCTACAGGAGGTGCCATCCCATCCTGGCAAATCCGCACAGTCAAGCTGATCCGCTATGTCAGC AACTGGGACTTCTTTATCGTTGGCTGTGAGGTCATCTTCTGCGTCTTCATCTTCTACTATGTGGTGGAA GAGATCCTGGAGCTCCACATTCACCGGCTTCGCTACCTCAGCAGCATCTGGAACATACTGGACCTGGTG GTCATCTTGCTCTCCATTGTGGCTGTGGGCTTCCACATATTCCGAACCCTCGAGGTGAATCGGCTCATG GGGAAGCTCCTGCAGCAGCCAAACACGTATGCAGACTTTGAGTTCCTCGCCTTCTGGCAGACACAGTAC AACAACATGAATGCTGTCAACCTCTTCTTCGCCTGGATCAAGATATTCAAGTACATCAGCTTCAACAAA ACCATGACCCAGCTCTCCTCCACGCTGGCCCGCTGTGCCAAGGACATCCTGGGCTTCGCCGTCATGTTC TTCATTGTTTTCTTCGCCTATGCCCAACTCGGCTACCTGCTTTTCGGGACCCAAGTGGAAAACTTTAGC ACTTTCATCAAGTGCATTTTCACTCAGTTCCGGATAATCCTCGGGGACTTTGACTACAATGCTATCGAC AATGCCAACCGCATCCTGGGCCCTGCCTACTTTGTCACCTATGTCTTCTTCGTCTTCTTCGTGCTCCTG AACATGTTCCTGGCCATCATCAATGACACATATTCAGAGGTCAAGGAGGAGCTGGCTGGACAGAAGGAT GAGCTGCAACTTTCTGACCTCCTGAAACAGGGCTACAACAAGACCCTACTAAGACTGCGTCTGAGGAAG GAGAGGGTTTCGGATGTGCAGAAGGTCCTGCAGGGTGGGGAGCAGGAGATCCAGTTTGAGGATTTCACC AACACCTTAAGGGAACTGGGACACGCAGAGCATGAAATCACTGAGCTCACGGCCACCTTCACCAAGTTT GACAGAGATGGGAATCGTATTCTGGATGAGAAGGAACAGGAAAAAATGCGACAGGACCTGGAGGAAGAG AGGGTGGCCCTCAACACTGAGATTGAGAAACTAGGCCGATCTATTGTGAGCAGCCCACAAGGCAAATCG GGTCCAGAGGCTGCCAGAGCAGGAGGCTGGGTTTCAGGAGAAGAATTCTACATGCTCACAAGGAGAGTT CTGCAGCTGGAGACTGTCCTGGAAGGAGTAGTGTCCCAGATTGATGCTGTAGGCTCAAAGCTGAAAATG CTGGAGAGGAAGGGGTGGCTGGCTCCCTCCCCAGGCGTGAAGGAACAAGCTATTTGGAAGCACCCGCAG CCAGCCCCAGCTGTGACCCCAGACCCCTGGGGAGTCCAGGGTGGGCAGGAGAGTGAGGTTCCCTATAAA AGAGAAGAGGAAGCCTTAGAGGAGAGGAGACTCTCCCGTGGTGAGATTCCAACGTTGCAGAGGAGTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253837
Insert Size	2277 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253837.1
RefSeq Size	3082 bp
RefSeq ORF	2277 bp
Locus ID	9033
UniProt ID	Q9P0L9
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW	86.9 kDa
Gene Summary	This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. This protein functions as a calcium-regulated nonselective cation channel. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of coding exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

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