TBPL1 (NM_001253676) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MGC:8389; MGC:9620; STUD; TLF; TLP; TRF2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332215 representing NM_001253676. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATGCAGACAGTGATGTTGCATTGGACATTCTAATTACAAATGTAGTCTGTGTTTTTAGAACAAGA TGTCATTTAAACTTAAGGAAGATTGCTTTGGAAGGAGCAAATGTAATTTATAAACGTGATGTTGGAAAA GTATTAATGAAGCTTAGAAAACCTAGAATTACAGCTACAATTTGGTCCTCAGGAAAAATTATTTGCACT GGAGCAACAAGTGAAGAAGAAGCTAAATTTGGTGCCAGACGCTTAGCCCGTAGTCTGCAGAAACTAGGT TTTCAGGTAATATTTACAGATTTTAAGGTTGTTAACGTTCTGGCAGTGTGTAACATGCCATTTGAAATC CGTTTGCCAGAATTCACAAAGAACAATAGACCTCATGCCAGTTACGAACCTGAACTTCATCCTGCTGTG TGCTATCGGATAAAATCTCTAAGAGCTACATTACAGATTTTTTCAACAGGAAGTATCACAGTAACAGGG CCCAATGTAAAGGCTGTTGCTACTGCTGTGGAACAGATTTACCCATTTGTGTTTGAAAGCAGGAAAGAA ATTTTATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253676
Insert Size	561 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253676.1
RefSeq Size	1635 bp
RefSeq ORF	561 bp
Locus ID	9519
UniProt ID	P62380
Protein Families	Transcription Factors
Protein Pathways	Basal transcription factors, Huntington's disease
MW	20.9 kDa
Gene Summary	This gene encodes a member of the TATA box-binding protein family. TATA box-binding proteins play a critical role in transcription by RNA polymerase II as components of the transcription factor IID (TFIID) complex. The encoded protein does not bind to the TATA box and initiates transcription from TATA-less promoters. This gene plays a critical role in spermatogenesis, and single nucleotide polymorphisms in this gene may be associated with male infertility. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 3. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

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