ISYNA1 (NM_001253389) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	INO1; INOS; IPS; IPS-1; IPS 1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332214 representing NM_001253389. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGGCGCCCAACGACCTCGTGTTCGATGGCTGGGACATCTCGTCGCTGAACCTGGCCGAGGCGATG CGGCGCGCGAAGGTGCTGGACTGGGGGCTGCAGGAGCAACTGTGGCCGCACATGGAGGCCCTGCGGCCC CGGCCTTCTGTTTACATCCCCGAATTCATCGCGGCCAACCAGAGCGCGCGCGCGGACAACCTCATCCCA GGCTCGCGTGCGCAGCAGCTGGAGCAGATCCGCAGGGACATCCGAGACTTCCGGTCTAGCGCGGGGCTG GACAAAGTCATAGTGCTGTGGACGGCGAACACGGAGCGCTTCTGTGAGGTGATTCCAGGCCTCAACGAC ACAGCCGAGAACCTGCTGCGCACCATTGAGCTCGGTCTGGAGGTGTCGCCCTCCACGCTCTTCGCCGTG GCCAGCATCCTGGAGGGCTGTGCCTTCCTCAATGGGTCTCCGCAGAACACCCTGGTGCCCGGAGCTCTT GAGCTCGCGTGGCAGCACCGGGTTTTTGTGGGCGGAGATGACTTCAAGTCAGGCCAGACCAAAGTCAAG TCCGTGCTTGTGGACTTCCTCATTGGCTCCGGCCTCAAGACCATGTCCATCGTGAGTTACAACCACCTG GGCAACAACGATGGGGAGAACCTATCGGCGCCATTGCAGTTCCGCTCTAAGGAGGTGTCCAAGAGCAAC GTGGTGGACGACATGGTGCAGAGCAACCCAGTGCTCTATACGCCCGGCGAAGAGCCTGACCACTGCGTG GTCATCAAGTATGTGCCGTACGTGGGTGACAGCAAGCGCGCGCTGGATGAGTATACCTCGGAGCTGATG CTGGGCGGAACCAACACACTGGTGCTGCACAACACGTGTGAGGACTCGCTGCTGGCCGCACCCATCATG CTGGACCTAGCGCTGCTGACCGAGCTGTGCCAGCGCGTGAGCTTCTGCACTGACATGGACCCCGAGCCG CAGACCTTCCACCCCGTGCTGTCCCTGCTCAGCTTCCTCTTCAAGGCGCCACTAGTGCCGCCCGGCAGC CCGGTGGTCAATGCGCTTTTCCGCCAGCGCAGCTGCATCGAGAACATCCTCAGGGCCTGCGTGGGGCTC CCGCCACAGAACCACATGCTCCTGGAACACAAAATGGAGCGCCCAGGGCCCAGCCTCAAGCGAGTTGGA CCCGTGGCTGCCACCTACCCTATGTTGAACAAGAAAGGACCGGTACCCGCTGCCACCAATGGCTGCACC GGTGATGCCAATGGGCATCTGCAAGAGGAGCCCCCAATGCCCACCACCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001253389
Insert Size	1293 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253389.1
RefSeq Size	2131 bp
RefSeq ORF	1293 bp
Locus ID	51477
UniProt ID	Q9NPH2
Protein Families	Druggable Genome
Protein Pathways	Inositol phosphate metabolism, Metabolic pathways
MW	47.1 kDa
Gene Summary	This gene encodes an inositol-3-phosphate synthase enzyme. The encoded protein plays a critical role in the myo-inositol biosynthesis pathway by catalyzing the rate-limiting conversion of glucose 6-phosphate to myoinositol 1-phosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus compared to isoform 1.

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