R Cadherin (CDH4) (NM_001252338) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAD4; R-CAD; RCAD
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332188 representing NM_001252338. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAACGGCAGCTGTGACTTCATTCTCAGCGCCTCCTTTCTACCCTGCTTTAAAGAAGTCAAGTTCAGC AGCTGTGTGGGGACCAAGGGGACACAATATGAGACCAACAGCATGGACTTCAAAGTTGGGGCAGATGGG ACAGTCTTCGCCACCCGGGAGCTGCAGGTCCCCTCCGAGCAGGTGGCGTTCACGGTGACTGCATGGGAC AGCCAGACAGCAGAGAAATGGGACGCCGTGGTGCGGTTGCTGGTGGCCCAGACCTCGTCCCCGCACTCT GGACACAAGCCGCAGAAAGGAAAGAAGGTCGTGGCTCTGGACCCCTCTCCGCCTCCGAAGGACACCCTG CTGCCGTGGCCCCAGCACCAGAACGCCAACGGGCTGAGGCGGCGCAAACGGGACTGGGTCATCCCGCCC ATCAACGTGCCCGAGAACTCGCGCGGGCCCTTCCCGCAGCAGCTCGTGAGGATCCGGTCCGACAAAGAC AATGACATCCCCATCCGGTACAGCATCACGGGAGTGGGCGCCGACCAGCCCCCCATGGAGGTCTTCAGC ATTGACTCCATGTCCGGCCGGATGTACGTCACAAGGCCCATGGACCGGGAGGAGCACGCCTCTTACCAC CTCCGAGCCCACGCTGTGGACATGAATGGCAACAAGGTGGAGAACCCCATCGACCTGTACATCTACGTC ATCGACATGAATGACAACCGCCCTGAGTTCATCAACCAGGTCTACAACGGCTCCGTGGACGAGGGCTCC AAGCCAGGCACCTACGTGATGACCGTCACGGCCAACGATGCTGACGACAGCACCACGGCCAACGGGATG GTGCGGTACCGGATCGTGACCCAGACCCCACAGAGCCCGTCCCAGAATATGTTCACCATCAACAGCGAG ACTGGAGATATCGTCACAGTGGCGGCTGGCCTGGACCGAGAGAAAGTTCAGCAGTACACAGTCATCGTT CAGGCCACAGATATGGAAGGAAATCTCAACTATGGCCTCTCAAACACAGCCACAGCCATCATCACGGTG ACAGATGTGAATGACAACCCGCCAGAATTTACCGCCAGCACGTTTGCAGGGGAGGTCCCCGAAAACCGC GTGGAGACCGTGGTCGCAAACCTCACGGTGATGGACCGAGATCAGCCCCACTCTCCAAACTGGAATGCC GTTTACCGCATCATCAGTGGGGATCCATCCGGGCACTTCAGCGTCCGCACAGACCCCGTAACCAACGAG GGCATGGTCACCGTGGTGAAGGCAGTCGACTACGAGCTCAACAGAGCTTTCATGCTGACAGTGATGGTG TCCAACCAGGCGCCCCTGGCCAGCGGAATCCAGATGTCCTTCCAGTCCACGGCAGGGGTGACCATCTCC ATCATGGACATCAACGAGGCTCCCTACTTCCCCTCAAACCACAAGCTGATCCGCCTGGAGGAGGGCGTG CCCCCCGGCACCGTGCTGACCACGTTTTCAGCTGTGGACCCTGACCGGTTCATGCAGCAGGCTGTGAGA TACTCAAAGCTGTCAGACCCAGCGAGCTGGCTGCACATCAATGCCACCAACGGCCAGATCACCACGGCG GCAGTGCTGGACCGTGAGTCCCTCTACACCAAAAACAACGTCTACGAGGCCACCTTCCTGGCAGCTGAC AATGGGATACCCCCGGCCAGCGGCACCGGGACCCTCCAGATCTATCTCATTGACATCAACGACAACGCC CCTGAGCTGCTGCCCAAGGAGGCGCAGATCTGCGAGAAGCCCAACCTGAACGCCATCAACATCACGGCG GCCGACGCTGACGTCGACCCCAACATCGGCCCCTACGTCTTCGAGCTGCCCTTTGTCCCGGCGGCCGTG CGGAAGAACTGGACCATCACCCGCCTGAACGGTGACTATGCCCAACTCAGCTTGCGCATCCTGTACCTG GAGGCCGGGATGTATGACGTCCCCATCATCGTCACAGACTCTGGAAACCCTCCCCTGTCCAACACGTCC ATCATCAAAGTCAAGGTGTGCCCATGTGATGACAACGGGGACTGCACCACCATTGGCGCAGTGGCAGCG GCTGGTCTGGGCACCGGTGCCATCGTGGCCATCCTCATCTGCATCCTCATCCTGCTGACCATGGTCCTG CTGTTTGTCATGTGGATGAAGCGGCGAGAGAAGGAGCGCCACACGAAGCAGCTGCTCATTGACCCCGAG GACGACGTCCGCGACAACATCCTCAAGTATGACGAGGAAGGCGGTGGCGAGGAGGACCAGGACTACGAC CTCAGCCAGCTGCAGCAGCCGGAAGCCATGGGGCACGTGCCAAGCAAAGCCCCTGGCGTGCGTCGCGTG GATGAGCGGCCGGTGGGCGCTGAGCCCCAGTACCCGATCAGGCCCATGGTGCCGCACCCAGGCGACATC GGTGACTTCATCAATGAGGGACTCCGCGCTGCTGACAACGACCCCACGGCACCCCCCTATGACTCCCTG CTGGTCTTCGACTACGAGGGGAGCGGCTCCACCGCAGGCTCCGTCAGCTCCCTGAACTCATCCAGTTCC GGGGACCAAGACTACGATTACCTCAACGACTGGGGGCCCAGATTCAAGAAGCTGGCGGACATGTATGGA GGTGGTGAAGAGGATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252338
Insert Size	2640 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252338.1
RefSeq Size	6343 bp
RefSeq ORF	2640 bp
Locus ID	1002
Protein Families	Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)
MW	96.4 kDa
Gene Summary	This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) has an alternate exon in place of the first two exons compared to variant 1, and the alternate exon has an in-frame translation start site. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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