TRPM1 (NM_001252024) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CSNB1C; LTRPC1; MLSN1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332165 representing NM_001252024. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGTCAGAAATCTTGGATAGAGAAAACCTTTTGCAAACGGGAATGTATCTTTGTAATTCCTAGCATG AAAGACTCTAACAGGTGTTGCTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAACA CCCAGCAAAAATGAAGAGGAAAGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCAC ACCCAGAGCTACCCAACAGATTCCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCC ATGTATATCCGTGTATCCTATGACACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAG CTGGAACTCCCCAAGCTCTTAATATCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTG AAACAAGTCTTTGGGAAAGGCCTGATCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGT GTCAGCACAGGTGTTATCAGCCACGTAGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGG GTTTGTGCTATAGGAATTGCTCCATGGGGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTA ACAAGAGTGTACCAGACCATGTCCAACCCTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCAC TTCATCCTGGCTGACAATGGCACCCTGGGCAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAA AAGCACATCTCCCTGCAGAAGATCAACACAAGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTG GAGGGGGGCCCTAACGTGGTGTCCATCGTCTTGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTG ATTTGTGATGGCAGCGGACGTGCCTCGGACATCCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGA ATAATAAATGAGTCCCTCAGGGAGCAGCTTCTAGTTACCATTCAGAAAACATTTAATTATAATAAGGCA CAATCACATCAGCTGTTTGCAATTATAATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGA ATGGGTTCTGAGGGCCAGCAGGACATCGAGATGGCAATTTTAACTGCCCTGCTGAAAGGAACAAACGTA TCTGCTCCAGATCAGCTGAGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTT GTCTTTGGGCCCCACTGGCCGCCCCTGGGAAGCCTGGCACCCCCGACGGACAGCAAAGCCACGGAGAAG GAGAAGAAGCCACCCATGGCCACCACCAAGGGAGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGGAAA GTGAAAGAGGAAGTGGAGGAAGAAACTGACCCCCGGAAGATAGAGCTGCTGAACTGGGTGAATGCTTTG GAGCAAGCGATGCTAGATGCTTTAGTCTTAGATCGTGTCGACTTTGTGAAGCTCCTGATTGAAAACGGA GTGAACATGCAACACTTTCTGACCATTCCGAGGCTGGAGGAGCTTTATAACACAAGACTGGGTCCACCA AACACACTTCATCTGCTGGTGAGGGATGTGAAAAAGAGCAACCTTCCGCCTGATTACCACATCAGCCTC ATAGACATCGGGCTCGTGCTGGAGTACCTCATGGGAGGAGCCTACCGCTGCAACTACACTCGGAAAAAC TTTCGGACCCTTTACAACAACTTGTTTGGACCAAAGAGGCCTAAAGCTCTTAAACTTCTGGGAATGGAA GATGATGAGCCTCCAGCTAAAGGGAAGAAAAAGAAAAAGAAGAAAAAGGAGGAAGAGATCGACATTGAT GTGGACGACCCTGCCGTGAGTCGGTTCCAGTATCCCTTCCACGAGCTGATGGTGTGGGCAGTGCTGATG AAACGCCAGAAAATGGCAGTGTTCCTCTGGCAGCGAGGGGAAGAGAGCATGGCCAAGGCCCTGGTGGCC TGCAAGCTCTACAAGGCCATGGCCCACGAGTCCTCCGAGAGTGATCTGGTGGATGACATCTCCCAGGAC TTGGATAACAATTCCAAAGACTTCGGCCAGCTTGCTTTGGAGTTATTAGACCAGTCCTATAAGCATGAC GAGCAGATCGCTATGAAACTCCTGACCTACGAGCTGAAAAACTGGAGCAACTCGACCTGCCTCAAACTG GCCGTGGCAGCCAAACACCGGGACTTCATTGCTCACACCTGCAGCCAGATGCTGCTGACCGATATGTGG ATGGGAAGACTGCGGATGCGGAAGAACCCCGGCCTGAAGGTTATCATGGGGATTCTTCTACCCCCCACC ATCTTGTTTTTGGAATTTCGCACATATGATGATTTCTCGTATCAAACATCCAAGGAAAATGAGGATGGC AAAGAAAAAGAAGAGGAAAATACGGATGCAAATGCAGATGCTGGCTCAAGAAAGGGGGATGAGGAGAAC GAGCACAAAAAACAGAGAAGTATTCCCATCGGAACAAAGATCTGTGAATTCTATAACGCGCCCATTGTC AAGTTCTGGTTTTACACAATATCATACTTGGGCTACCTGCTGCTGTTTAACTACGTCATCCTGGTGCGG ATGGATGGCTGGCCGTCCCTCCAGGAGTGGATCGTCATCTCCTACATCGTGAGCCTGGCGTTAGAGAAG ATACGAGAGATCCTCATGTCAGAACCAGGCAAACTCAGCCAGAAAATCAAAGTTTGGCTTCAGGAGTAC TGGAACATCACAGATCTCGTGGCCATTTCCACATTCATGATTGGAGCAATTCTTCGCCTACAGAACCAG CCCTACATGGGCTATGGCCGGGTGATCTACTGTGTGGATATCATCTTCTGGTACATCCGTGTCCTGGAC ATCTTTGGTGTCAACAAGTATCTGGGGCCATACGTGATGATGATTGGAAAGATGATGATCGACATGCTG TACTTTGTGGTCATCATGCTGGTCGTGCTCATGAGTTTCGGAGTAGCCCGTCAAGCCATTCTGCATCCA GAGGAGAAGCCCTCTTGGAAACTGGCCCGAAACATCTTCTACATGCCCTACTGGATGATCTATGGAGAG GTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATTAATCCTCCTTGTGGTGAGAACCTATATGAT GAGGAGGGCAAGCGGCTTCCTCCCTGTATCCCCGGCGCCTGGCTCACTCCAGCACTCATGGCGTGCTAT CTACTGGTCGCCAACATCCTGCTGGTGAACCTGCTGATTGCTGTGTTCAACAATACCTTCTTTGAAGTA AAATCAATATCCAACCAGGTGTGGAAGTTCCAGCGATATCAGCTGATTATGACATTTCATGACAGGCCA GTCCTGCCCCCACCGATGATCATTTTAAGCCACATCTACATCATCATTATGCGTCTCAGCGGCCGCTGC AGGAAAAAGAGAGAAGGGGACCAAGAGGAACGGGATCGTGGATTGAAGCTCTTCCTTAGCGACGAGGAG CTAAAGAGGCTGCATGAGTTCGAGGAGCAGTGCGTGCAGGAGCACTTCCGGGAGAAGGAGGATGAGCAG CAGTCGTCCAGCGACGAGCGCATCCGGGTCACTTCTGAAAGAGTTGAAAATATGTCAATGAGGTTGGAA GAAATCAATGAAAGAGAAACTTTTATGAAAACTTCCCTGCAGACTGTTGACCTTCGACTTGCTCAGCTA GAAGAATTATCTAACAGAATGGTGAATGCTCTTGAAAATCTTGCGGGAATCGACAGGTCTGACCTGATC CAGGCACGGTCCCGGGCTTCTTCTGAATGTGAGGCAACGTATCTTCTCCGGCAAAGCAGCATCAATAGC GCTGATGGCTACAGCTTGTATCGATATCATTTTAACGGAGAAGAGTTATTATTTGAGGATACATCTCTC TCCACGTCACCAGGGACAGGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAAGGAAGAGAAGGACGTG AAAACGCACCTAGTCCCAGAATGTCAGAACAGTCTTCACCTTTCACTGGGCACAAGCACATCAGCAACC CCAGATGGCAGTCACCTTGCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAATTAGGTCCAGATATT GGGATTTCAAAGGAAGATGATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTATTTCCCCAAGTTTA AATAAAACAGATGTGATACATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTAACAGTGGAAACG ACAAATATAGAAGGCACTATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATTTCCCCGATGAA ACGATCAATGCTTGTAAAACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAAGCTGGTCGGT GGGGTTAACCAGGATGTAGAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGGCAATGCCAA GTTCAAAAGATCACGCGCTCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAGTGCAAGCT GAGCATAAAGAGCAGTTTGCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCGAATCCCT CGCTTGTCCCTAACCATTACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGATCAAACT TTGGGATTCCCATCTCTCAGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTCAGGGA AAGTTAGACAGATCTGGACATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGCAGAA GAAAAAAAGGTTAAGAAAGAGAAAGCTTCCACAGAAACTGAATGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001252024
Insert Size	4878 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252024.1
RefSeq Size	5810 bp
RefSeq ORF	4878 bp
Locus ID	4308
UniProt ID	Q7Z4N2
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW	184.8 kDa
Gene Summary	This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate exon in the 5' coding region. These differences cause translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.

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