PAPOLA (NM_001252007) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PAP; PAP-alpha
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332163 representing NM_001252007. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGTTTCCAGTTACAACACAGGGATCACAACAAACACAACCGCCACAGAAGCACTATGGCATTACT TCTCCTATCAGCTTAGCAGCCCCCAAGGAGACTGACTGCGTACTTACACAGAAACTAATTGAGACATTG AAACCCTTTGGGGTTTTTGAAGAGGAAGAGGAACTGCAGCGCAGGATTTTAATTTTGGGAAAACTAAAT AACCTGGTAAAAGAGTGGATACGAGAAATCAGTGAAAGCAAGAATCTTCCACAATCTGTAATTGAAAAT GTTGGAGGAAAAATTTTTACATTTGGATCTTACAGATTAGGAGTGCATACAAAAGGTGCTGATATTGAT GCGTTGTGTGTTGCACCAAGACATGTTGATCGAAGTGACTTTTTCACCTCATTCTATGATAAGTTGAAA TTACAGGAAGAAGTAAAAGATTTAAGAGCTGTTGAAGAGGCATTCGTACCAGTTATTAAACTCTGTTTT GATGGGATAGAGATTGATATTTTGTTTGCAAGATTAGCACTGCAGACAATTCCTGAAGATTTGGATCTA CGAGATGACAGTCTGCTAAAAAATTTAGATATAAGATGTATAAGAAGTCTTAACGGTATGAGAAAGCCT ACTTCCTTTTGTGTACTTCAGTTTTTGTCAGATATTAGTTGTTTTTACACAAGTTTTGTATTGAAGCTT TTTATAGCTATATTGTTAACACAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001252007
Insert Size	717 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252007.1
RefSeq Size	4847 bp
RefSeq ORF	717 bp
Locus ID	10914
UniProt ID	P51003
Protein Families	Transcription Factors
Protein Pathways	RNA degradation
MW	27.1 kDa
Gene Summary	The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (3) lacks multiple exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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