DIP13B (APPL2) (NM_001251905) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DIP13B
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332147 representing NM_001251905. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAGCGCGTCTATGGAGCCCAGAATGAGATGTGCCTGGCCACACAACAGCTTTCTAAGCAACTGCTG GCATATGAAAAACAGAACTTTGCTCTTGGCAAAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTT TCCAAAGTGGTGGATGAGCTTAATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTT CTACCTATCATACAATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTC GCTAGCAATGAGCATGACCTCTCAATGGCAAAATACAGCAGGCTGCCTAAGAAAAAGGAGAATGAGAAG GTGAAGACCGAAGTCGGAAAAGAGGTGGCCGCGGCCCGGCGGAAGCAGCACCTCTCCTCCCTTCAGTAC TACTGTGCCCTCAACGCGCTGCAGTACAGAAAGCAAATGGCCATGATGGAGCCCATGATAGGCTTTGCC CATGGACAGATTAACTTTTTTAAGAAGGGAGCAGAGATGTTTTCCAAACGTATGGACAGCTTTTTATCC TCCGTTGCAGACATGGTTCAAAGCATTCAGGTAGAACTGGAAGCCGAGGCGGAAAAGATGCGGGTGTCC CAGCAAGAATTACTTTCTGTTGATGAATCTGTTTACACTCCAGACTCTGATGTGGCCGCACCACAGATC AACAGGAACCTCATCCAGAAGGCTGGTTACCTTAATCTTAGAAACAAAACAGGGCTGGTCACCACCACC TGGGAGAGGCTTTATTTCTTCACCCAAGGCGGGAATCTCATGTGTCAGCCCAGGGGAGCCGTGGCTGGA GGTTTGATCCAGGACCTGGACAACTGCTCAGTGATGGCCGTGGATTGCGAAGACCGGCGCTACTGCTTC CAGATCACCACGCCCAATGGAAAATCGGGAATAATCCTCCAGGCTGAGAGCAGAAAGGAAAATGAAGAG TGGATATGTGCAATAAACAACATCTCCAGACAGATCTACCTGACCGACAACCCTGAGGCAGTCGCGATC AAGTTGAATCAGACCGCTCTGCAAGCAGTGACTCCCATTACAAGTTTTGGAAAAAAACAAGAAAGCTCA TGCCCCAGCCAGAACCTGAAAAATTCAGAGATGGAAAATGAAAATGACAAGATTGTTCCCAAAGCAACA GCCAGTCTACCTGAAGCAGAGGAGCTGATCGCGCCTGGAACGCCGATTCAATTCGATATTGTGCTTCCT GCTACAGAATTCCTTGATCAGAACAGAGGGAGCAGGCGTACCAACCCTTTTGGTGAAACTGAGGATGAA TCATTTCCAGAAGCAGAAGATTCTCTTTTGCAGCAGATGTTTATAGTTCGGTTTTTGGGATCAATGGCA GTTAAAACAGACAGCACTACTGAAGTGATTTATGAAGCGATGAGACAAGTATTGGCTGCTCGGGCTATT CATAACATCTTCCGCATGACAGAATCCCATCTGATGGTCACCAGTCAATCTTTGAGGTTGATAGATCCA CAGACTCAAGTATCAAGGGCCAATTTTGAACTTACCAGTGTCACACAATTTGCTGCTCATCAAGAAAAC AAGAGACTGGTTGGTTTTGTCATCCGTGTTCCTGAATCCACTGGAGAAGAATCTCTGAGTACATACATT TTTGAAAGCAACTCAGAAGGCGAAAAGATATGTTATGCTATTAATTTGGGAAAAGAAATTATTGAGGTT CAGAAGGATCCAGAAGCACTGGCTCAATTAATGCTGTCCATACCACTAACCAATGACGGAAAATATGTA CTGTTAAACGATCAACCAGATGACGATGATGGAAATCCAAACGAACATAGAGGCGCAGAATCCGAAGCA TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001251905
Insert Size	1866 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001251905.1
RefSeq Size	3332 bp
RefSeq ORF	1866 bp
Locus ID	55198
UniProt ID	Q8NEU8
MW	69.7 kDa
Gene Summary	The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (3) differs in the 5' exon, which results in a downstream AUG start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1.

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