TRPC1 (NM_001251845) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HTRP-1; TRP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332135 representing NM_001251845. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATGGCGGCCCTGTACCCGAGCACGGACCTCTCGGGCGCCTCCTCCTCCTCCCTGCCTTCCTCTCCA TCCTCTTCCTCGCCGAACGAGGTGATGGCGCTGAAGGATGTGCGGGAGGTGAAGGAGGAGAATACGCTG AATGAGAAGCTTTTCTTGCTGGCGTGCGACAAGGGTGACTATTATATGGTTAAAAAGATTTTGGAGGAA AACAGTTCAGGTGACTTGAACATAAATTGCGTAGATGTGCTTGGGAGAAATGCTGTTACCATAACTATT GAAAACGAAAACTTGGATATACTGCAGCTTCTTTTGGACTACGGTTGTCAGTCTGCAGATGCACTTTTG GTGGCAATCGACTCTGAAGTAGTGGGAGCTGTTGATATACTACTTAATCATCGACCAAAACGATCATCA AGACCAACTATAGTAAAACTAATGGAACGAATTCAGAATCCTGAGTATTCAACAACTATGGATGTTGCA CCTGTCATTTTAGCTGCTCATCGTAACAACTATGAAATTCTTACAATGCTCTTAAAACAGGATGTATCT CTACCCAAGCCCCATGCAGTTGGCTGTGAATGCACATTGTGTTCTGCAAAAAACAAAAAGGATAGCCTC CGGCATTCCAGGTTTCGTCTTGATATATATCGATGTTTGGCCAGTCCAGCTCTAATAATGTTAACAGAG GAGGATCCAATTCTGAGAGCATTTGAACTTAGTGCTGATTTAAAAGAACTAAGTCTTGTGGAGGTGGAA TTCAGGAATGATTATGAGGAACTAGCCCGGCAATGTAAAATGTTTGCTAAGGATTTACTTGCACAAGCC CGGAATTCTCGTGAATTGGAAGTTATTCTAAACCATACGTCTAGTGACGAGCCTCTTGACAAACGGGGA TTATTAGAAGAAAGAATGAATTTAAGTCGTCTAAAACTTGCTATCAAATATAACCAGAAAGAGTTTGTC TCCCAGTCTAACTGCCAGCAGTTCCTGAACACTGTTTGGTTTGGACAGATGTCGGGTTACCGACGCAAG CCCACCTGTAAGAAGATAATGACTGTTTTGACAGTAGGCATCTTTTGGCCAGTTTTGTCACTTTGTTAT TTGATAGCTCCCAAATCTCAGTTTGGCAGAATCATTCACACACCTTTTATGAAATTTATCATTCATGGA GCATCATATTTCACATTTCTGCTGTTGCTTAATCTATACTCTCTTGTCTACAATGAGGATAAGAAAAAC ACAATGGGGCCAGCCCTTGAAAGAATAGACTATCTTCTTATTCTGTGGATTATTGGGATGATTTGGTCA GACATTAAAAGACTCTGGTATGAAGGGTTGGAAGACTTTTTAGAAGAATCTCGTAATCAACTCAGTTTT GTCATGAATTCTCTTTATTTGGCAACCTTTGCCCTCAAAGTGGTTGCTCACAACAAGTTTCATGATTTT GCTGATCGGAAGGATTGGGATGCATTCCATCCTACACTGGTGGCAGAAGGGCTTTTTGCATTTGCAAAT GTTCTAAGTTATCTTCGTCTCTTTTTTATGTATACAACCAGCTCTATCTTGGGTCCATTACAGATTTCA ATGGGACAGATGTTACAAGATTTTGGAAAATTTCTTGGGATGTTTCTTCTTGTTTTGTTTTCTTTCACA ATTGGACTGACACAACTGTATGATAAAGGATATACTTCAAAGGAGCAGAAGGACTGTGTAGGCATCTTC TGTGAACAGCAAAGCAATGATACCTTCCATTCGTTCATTGGCACCTGCTTTGCTTTGTTCTGGTATATT TTCTCCTTAGCGCATGTGGCAATCTTTGTCACAAGATTTAGCTATGGAGAAGAACTGCAGTCCTTTGTG GGAGCTGTCATTGTTGGTACATACAATGTCGTGGTTGTGATTGTGCTTACCAAACTGCTGGTGGCAATG CTTCATAAAAGCTTTCAGTTGATAGCAAATCATGAAGACAAAGAATGGAAGTTTGCTCGAGCAAAATTA TGGCTTAGCTACTTTGATGACAAATGTACGTTACCTCCACCTTTCAACATCATTCCCTCACCAAAGACT ATCTGCTATATGATTAGTAGCCTCAGTAAGTGGATTTGCTCTCATACATCAAAAGGCAAGGTCAAACGG CAAAACAGTTTAAAGGAATGGAGGAATTTGAAACAGAAGAGAGATGAAAACTATCAAAAAGTGATGTGC TGCCTAGTGCATCGTTACTTGACTTCCATGAGACAGAAGATGCAAAGTACAGATCAGGCAACTGTGGAA AATCTAAACGAACTGCGCCAAGATCTGTCAAAATTCCGAAATGAAATAAGGGATTTACTTGGCTTTCGG ACTTCTAAATATGCTATGTTTTATCCAAGAAATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001251845
Insert Size	2382 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001251845.1
RefSeq Size	4179 bp
RefSeq ORF	2382 bp
Locus ID	7220
UniProt ID	P48995
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Protein Pathways	Calcium signaling pathway, Huntington's disease, Parkinson's disease
MW	91.2 kDa
Gene Summary	The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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