PRPF4 (NM_001244926) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332103 representing NM_001244926. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTTCCTCGCGAGCCTCTTCCACGGCAACCAAAACTAAAGCACCCGACGACTTAGTTGCTCCGGTC GTGAAGAAACCACACATCTATTATGGAAGTTTGGAAGAGAAGGAGAGGGAGCGTCTGGCCAAAGGAGAG TCTGGGATTTTGGGGAAAGACGGACTTAAAGCAGGGATCGAAGCTGGAAATATTAATATAACCTCTGGA GAAGTGTTTGAAATTGAAGAGCATATCAGCGAGCGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAGG AAGCGAGCCCGGCAGATCAATGTTTCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAGAGCCTTGGGG GAACCCATCACACTTTTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTTAAGAAATATCCTCTCAGTT GTCGGTACTGATGCCTTGAAAAAGACCAAAAAGGATGATGAGAAGTCTAAAAAGTCCAAAGAAGAGTAT CAGCAAACCTGGTATCATGAAGGACCAAATAGCTTGAAGGTGGCAAGACTATGGATTGCTAATTATTCG TTGCCCAGGGCAATGAAACGCTTGGAAGAGGCCCGACTCCATAAGGAGATTCCTGAGACAACAAGGACC TCCCAGATGCAAGAGCTGCACAAGTCTCTCCGGTCTTTGAATAATTTTTGCAGTCAGATTGGGGATGAT CGGCCTATCTCCTACTGTCACTTTAGTCCCAATTCCAAGATGCTGGCCACAGCTTGTTGGAGTGGGCTT TGCAAGCTCTGGTCTGTTCCTGATTGCAACCTCCTTCACACTCTTCGAGGGCATAACACAAATGTAGGA GCAATTGTATTCCATCCCAAATCCACTGTCTCCTTGGACCCAAAAGATGTCAACCTGGCCTCTTGTGCG GCTGATGGCTCTGTGAAGCTTTGGAGTCTCGACAGTGATGAACCAGTGGCAGATATTGAAGGCCATACA GTGCGTGTGGCGCGGGTAATGTGGCATCCTTCAGGACGTTTCCTGGGCACCACCTGCTATGACCGTTCA TGGCGCTTATGGGATTTGGAGGCTCAAGAGGAGATCCTGCATCAGGAAGGCCATAGCATGGGTGTGTAT GACATTGCCTTCCATCAAGATGGCTCTTTGGCTGGCACTGGGGGACTGGATGCATTTGGTCGAGTTTGG GACCTACGCACAGGACGTTGTATCATGTTCTTAGAAGGCCACCTGAAAGAAATCTATGGAATAAATTTC TCCCCCAATGGCTATCACATTGCAACCGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTCCGACAG CGGCGTTGCGTCTACACCATCCCTGCTCATCAGAACTTAGTGACTGGTGTCAAGTTTGAGCCTATCCAT GGGAACTTCTTGCTTACTGGTGCCTATGATAACACAGCCAAGATCTGGACGCACCCAGGCTGGTCCCCG CTGAAGACTCTGGCTGGCCACGAAGGCAAAGTGATGGGCCTAGATATTTCTTCCGATGGGCAGCTCATA GCCACTTGCTCATATGACAGGACCTTCAAGCTGTGGATGGCTGAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001244926
Insert Size	1566 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001244926.1
RefSeq Size	2938 bp
RefSeq ORF	1566 bp
Locus ID	9128
UniProt ID	O43172
Protein Pathways	Spliceosome
MW	58.3 kDa
Gene Summary	The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1.

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