KIAA0586 (NM_001244193) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JBTS23; SRTD14; Talpid3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332070 representing NM_001244193. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGATGCAGGCATAGAGAAGGATGCTGTTACT CAGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACAACCGTGGCTGCAGCAACTGCTGCTGCC ATTGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTGGAAGCAAAAGTCAATTCTGTTACAGAA TTACTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGTGTTACAGAGCAGCAAACAAGCATTCAG AGGAAACAAGAGAAATTACATTGTCATGATCACGAAAAGCAAATGAATGTGTTTATGGAGCAGCACATA AGGCATCTTGAAAAGTTACAACAACAACAAATAGATATTCAGACTCATTTTATTAGTGCTGCACTCAAG ACTAGTAGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCAGTGGAAAAGTATTCCGTAAAACCAGAA CACCCTAATCTTGGTAGCTGTAATCCATCTTTATATAACACATTTGCTTCCAAACAAGCACCTTTAAAA GAAGTTGAAGATACGAGTTTTGATAAACAGAAATCTCCTTTGGAGACACCAGCACCTCGCAGATTTGCT CCTGTACCTGTTTCAAGGGATGATGAACTATCAAAGAGGGAAAATCTTTTGGAAGAAAAAGAAAATATG GAAGTGTCGTGTCACAGAGGAAATGTAAGACTATTGGAACAAATTTTGAATAATAATGATTCTTTGACA AGAAAAAGTGAATCATCAAACACCACCTCACTAACTAGGTCAAAAATAGGATGGACTCCTGAGAAAACA AACAGATTTCCTTCCTGTGAAGAGCTAGAAACAACTAAAGTGACTATGCAGAAGTCTGATGATGTTCTT CATGACCTTGGCCAAAAAGAGAAAGAAACAAATAGCATGGTCCAGCCAAAAGAATCTCTGAGTATGTTG AAGCTTCCAGATCTTCCACAGAATTCTGTTAAGCTTCAAACAACCAATACAACAAGATCTGTATTGAAA GATGCTGAGAAGATTTTGAGAGGAGTACAAAACAATAAAAAAGTACTTGAAGAAAACCTGGAAGCTATT ATTCGTGCAAAAGATGGAGCTGCCATGTATTCGCTTATCAATGCTTTATCTACCAACAGAGAGATGTCA GAGAAAATTAGGATCAGAAAGACAGTGGATGAATGGATTAAAACTATTTCTGCAGAAATTCAGGATGAA CTGTCAAGAACAGATTATGAACAAAAAAGATTTGATCAGAAGAATCAGAGAACCAAGAAAGGTCAGAAT ATGACTAAAGATATTAGAACCAACACACAAGATAAAACTGTCAACAAATCTGTAATTCCAAGAAAACAT TCTCAAAAGCAAATAGAAGAGCATTTTAGAAATCTACCTATGAGGGGCATGCCTGCTTCAAGTTTACAG AAAGAGAGAAAGGAAGGGCTTTTGAAAGCAACCACAGTAATACAAGATGAAGATTATATGTTACAAGTC TATGGAAAGCCAGTTTATCAGGGCCATCGAAGCACTCTTAAAAAAGGACCATATCTCAGATTTAATTCT CCATCTCCTAAGTCCAGACCACAGAGACCAAAAGTAATAGAACGAGTTAAAGGCACTAAGGTAAAGTCA ATAAGAACACAGACTGACTTCTATGCAACAAAACCTAAGAAGATGGATTCTAAAATGAAACATTCTGTT CCTGTGTTACCTCATGGCGATCAGCAATATTTGTTCAGCCCAAGTAGAGAAATGCCTACTTTTTCAGGT ACATTGGAAGGTCATCTGATTCCTATGGCAATTCTTTTAGGACAAACCCAAAGTAATAGTGATACCATG CCACCTGCTGGAGTGATTGTCAGCAAGCCACACCCTGTAACTGTGACTACTTCTATTCCTCCATCATCT CGAAAAGTAGAAACTGGAGTAAAGAAACCTAACATAGCCATTGTAGAAATGAAGTCAGAAAAAAAGGAT CCTCCTCAGCTTACTGTGCAGGTATTACCCAGTGTAGATATTGACAGCATTTCAAATAGTAGTGCTGAT GTCCTTTCACCTCTGTCTAGCCCCAAAGAAGCATCTCTTCCTCCTGTGCAAACTTGGATAAAGACTCCA GAAATTATGAAGGTAGATGAAGAAGAGGTGAAGTTTCCAGGAACTAACTTTGATGAAATAATCGATGTC ATACAGGAAGAAGAAAAATGTGATGAAATTCCAGACTCTGAACCAATTCTGGAGTTTAACAGAAGTGTT AAAGCTGATTCTACAAAATATAATGGTCCTCCATTTCCGCCAGTTGCTTCTACTTTTCAGCCCACTGCT GATATTCTGGATAAAGTAATTGAGAGAAAAGAAACACTGGAAAATAGCTTAATTCAATGGGTAGAGCAA GAAATAATGTCAAGAATTATCTCTGGGCTCTTTCCAGTCCAGCAACAGATTGCACCTAGTATCAGTGTT TCAGTCAGTGAGACAAGTGAACCACTGACTTCTGACATTGTGGAAGGAACAAGCAGTGGCGCCCTCCAG CTTTTTGTTGATGCTGGTGTTCCTGTGAACTCAAATGTGATTAAACATTTTGTTAACGAAGCTCTTGCT GAGACCATTGCTGTCATGCTGGGTGACAGAGAAGCAAAGAAGCAAGGTCCTGTTGCTACAGGTGTTTCT GGGGATGCTTCAACAAATGAAACATATTTGCCGGCAAGAGTGTGCACCCCACTGCCTACCCCACAGCCT ACGCCTCCTTGCTCACCTTCATCACCTGCTAAGGAGTGTGTTTTGGTAAAGACTCCAGATTCTTCTCCC TGTGATTCGGATCATGATATGGCTTTTCCTGTGAAAGAAATATGTGCTGAAAAAGGAGATGATATGCCT GCCATCATGCTTGTTAATACTCCAACAGTTACCCCTACTACTACACCTCCTCCAGCGGCGGCAGTTTTT ACCCCAACTTTGTCAGATATTTCCATTGATAAATTGAAGGTATCAAGCCCAGAGCTTCCCAAGCCATGG GGTGATGGAGACCTGCCACTGGAAGAAGAGAACCCTAACTCACCTCAAGAAGAACTTCATCCAAGAGCT ATTGTAATGTCTGTGGCTAAGGATGAAGAACCAGAGAGTATGGATTTCCCTGCTCAGCCTCCACCTCCA GAGCCAGTTCCCTTTATGCCATTTCCTGCCGGCACCAAGGCCCCTTCCCCCTCACAGATGCCAGGTTCT GATTCATCAACACTGGAGAGCACATTGAGTGTTACTGTCACTGAAACTGAAACTTTAGATAAACCCATC TCTGAAGGAGAGATTTTATTTAGCTGTGGTCAAAAATTGGCCCCCAAGATTTTAGAAGATATAGGACTG TACCTGACAAACCTTAATGATAGCTTATCCAGCACTCTGCATGATGCCGTTGAAATGGAGGATGATCCT CCTAGTGAAGGGCAAGTGATTAGGATGTCCCATAAAAAATTTCATGCAGATGCAATTCTTTCTTTTGCT AAACAAAACCAGGAGTCAGCAGTTTCCCAGCAAGCAGTCTATCATTCAGAGGACTTGGAAAACAGTGTG GGTGAACTTAGTGAAGGACAAAGACCCCAGCTAACAGCGGCAGCAGAGAACATCTTAATGGGACATTCT CTCTATATGCAGCCACCTGTCACTAATACACAGTCTTTGGATCAACAATGTGATCCTAAACCATTATCT CGGCAATTTGACACAGTTTCAGGTAGTATTTATGAAGATTCATGTGCTAGTCATGGTCCAATGAGTTTG GGAGAATTGGAGTTGGAGCCAAATTCTAAGCTGGTTCTTCCCACAACACTTCTGACAGCACAAGAAAAT GATGTTAATTTACCAGTAGCCGCTGAAGATTTTTCCCAGTACCAACTAAAGCAAAATCAGGATGTTAAG CAAGTTGAACACAAACCATCACAAAGTTACCTACGTGTTAGAAATAAATCTGATATTGCACCTTCACAG CAACAAGTTTTGATCAGCAGGATAAAGAATTGTAAGAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001244193
Insert Size	4044 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001244193.1
RefSeq Size	4331 bp
RefSeq ORF	4044 bp
Locus ID	9786
UniProt ID	Q9BVV6
MW	149.2 kDa
Gene Summary	This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (6) differs in both UTRs and contains multiple differences in the 5' and 3' coding regions, compared to variant 1. The resulting protein (isoform 6) has a shorter N-terminus and a distinct C-terminus.

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