KIAA0586 (NM_001244193) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	JBTS23; SRTD14; Talpid3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235274 representing NM_001244193 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGATGCAGGCATAGAGAAGGATGCTGTTACTC AGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACAACCGTGGCTGCAGCAACTGCTGCTGCCAT TGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTGGAAGCAAAAGTCAATTCTGTTACAGAATTA CTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGTGTTACAGAGCAGCAAACAAGCATTCAGAGGA AACAAGAGAAATTACATTGTCATGATCACGAAAAGCAAATGAATGTGTTTATGGAGCAGCACATAAGGCA TCTTGAAAAGTTACAACAACAACAAATAGATATTCAGACTCATTTTATTAGTGCTGCACTCAAGACTAGT AGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCAGTGGAAAAGTATTCCGTAAAACCAGAACACCCTA ATCTTGGTAGCTGTAATCCATCTTTATATAACACATTTGCTTCCAAACAAGCACCTTTAAAAGAAGTTGA AGATACGAGTTTTGATAAACAGAAATCTCCTTTGGAGACACCAGCACCTCGCAGATTTGCTCCTGTACCT GTTTCAAGGGATGATGAACTATCAAAGAGGGAAAATCTTTTGGAAGAAAAAGAAAATATGGAAGTGTCGT GTCACAGAGGAAATGTAAGACTATTGGAACAAATTTTGAATAATAATGATTCTTTGACAAGAAAAAGTGA ATCATCAAACACCACCTCACTAACTAGGTCAAAAATAGGATGGACTCCTGAGAAAACAAACAGATTTCCT TCCTGTGAAGAGCTAGAAACAACTAAAGTGACTATGCAGAAGTCTGATGATGTTCTTCATGACCTTGGCC AAAAAGAGAAAGAAACAAATAGCATGGTCCAGCCAAAAGAATCTCTGAGTATGTTGAAGCTTCCAGATCT TCCACAGAATTCTGTTAAGCTTCAAACAACCAATACAACAAGATCTGTATTGAAAGATGCTGAGAAGATT TTGAGAGGAGTACAAAACAATAAAAAAGTACTTGAAGAAAACCTGGAAGCTATTATTCGTGCAAAAGATG GAGCTGCCATGTATTCGCTTATCAATGCTTTATCTACCAACAGAGAGATGTCAGAGAAAATTAGGATCAG AAAGACAGTGGATGAATGGATTAAAACTATTTCTGCAGAAATTCAGGATGAACTGTCAAGAACAGATTAT GAACAAAAAAGATTTGATCAGAAGAATCAGAGAACCAAGAAAGGTCAGAATATGACTAAAGATATTAGAA CCAACACACAAGATAAAACTGTCAACAAATCTGTAATTCCAAGAAAACATTCTCAAAAGCAAATAGAAGA GCATTTTAGAAATCTACCTATGAGGGGCATGCCTGCTTCAAGTTTACAGAAAGAGAGAAAGGAAGGGCTT TTGAAAGCAACCACAGTAATACAAGATGAAGATTATATGTTACAAGTCTATGGAAAGCCAGTTTATCAGG GCCATCGAAGCACTCTTAAAAAAGGACCATATCTCAGATTTAATTCTCCATCTCCTAAGTCCAGACCACA GAGACCAAAAGTAATAGAACGAGTTAAAGGCACTAAGGTAAAGTCAATAAGAACACAGACTGACTTCTAT GCAACAAAACCTAAGAAGATGGATTCTAAAATGAAACATTCTGTTCCTGTGTTACCTCATGGCGATCAGC AATATTTGTTCAGCCCAAGTAGAGAAATGCCTACTTTTTCAGGTACATTGGAAGGTCATCTGATTCCTAT GGCAATTCTTTTAGGACAAACCCAAAGTAATAGTGATACCATGCCACCTGCTGGAGTGATTGTCAGCAAG CCACACCCTGTAACTGTGACTACTTCTATTCCTCCATCATCTCGAAAAGTAGAAACTGGAGTAAAGAAAC CTAACATAGCCATTGTAGAAATGAAGTCAGAAAAAAAGGATCCTCCTCAGCTTACTGTGCAGGTATTACC CAGTGTAGATATTGACAGCATTTCAAATAGTAGTGCTGATGTCCTTTCACCTCTGTCTAGCCCCAAAGAA GCATCTCTTCCTCCTGTGCAAACTTGGATAAAGACTCCAGAAATTATGAAGGTAGATGAAGAAGAGGTGA AGTTTCCAGGAACTAACTTTGATGAAATAATCGATGTCATACAGGAAGAAGAAAAATGTGATGAAATTCC AGACTCTGAACCAATTCTGGAGTTTAACAGAAGTGTTAAAGCTGATTCTACAAAATATAATGGTCCTCCA TTTCCGCCAGTTGCTTCTACTTTTCAGCCCACTGCTGATATTCTGGATAAAGTAATTGAGAGAAAAGAAA CACTGGAAAATAGCTTAATTCAATGGGTAGAGCAAGAAATAATGTCAAGAATTATCTCTGGGCTCTTTCC AGTCCAGCAACAGATTGCACCTAGTATCAGTGTTTCAGTCAGTGAGACAAGTGAACCACTGACTTCTGAC ATTGTGGAAGGAACAAGCAGTGGCGCCCTCCAGCTTTTTGTTGATGCTGGTGTTCCTGTGAACTCAAATG TGATTAAACATTTTGTTAACGAAGCTCTTGCTGAGACCATTGCTGTCATGCTGGGTGACAGAGAAGCAAA GAAGCAAGGTCCTGTTGCTACAGGTGTTTCTGGGGATGCTTCAACAAATGAAACATATTTGCCGGCAAGA GTGTGCACCCCACTGCCTACCCCACAGCCTACGCCTCCTTGCTCACCTTCATCACCTGCTAAGGAGTGTG TTTTGGTAAAGACTCCAGATTCTTCTCCCTGTGATTCGGATCATGATATGGCTTTTCCTGTGAAAGAAAT ATGTGCTGAAAAAGGAGATGATATGCCTGCCATCATGCTTGTTAATACTCCAACAGTTACCCCTACTACT ACACCTCCTCCAGCGGCGGCAGTTTTTACCCCAACTTTGTCAGATATTTCCATTGATAAATTGAAGGTAT CAAGCCCAGAGCTTCCCAAGCCATGGGGTGATGGAGACCTGCCACTGGAAGAAGAGAACCCTAACTCACC TCAAGAAGAACTTCATCCAAGAGCTATTGTAATGTCTGTGGCTAAGGATGAAGAACCAGAGAGTATGGAT TTCCCTGCTCAGCCTCCACCTCCAGAGCCAGTTCCCTTTATGCCATTTCCTGCCGGCACCAAGGCCCCTT CCCCCTCACAGATGCCAGGTTCTGATTCATCAACACTGGAGAGCACATTGAGTGTTACTGTCACTGAAAC TGAAACTTTAGATAAACCCATCTCTGAAGGAGAGATTTTATTTAGCTGTGGTCAAAAATTGGCCCCCAAG ATTTTAGAAGATATAGGACTGTACCTGACAAACCTTAATGATAGCTTATCCAGCACTCTGCATGATGCCG TTGAAATGGAGGATGATCCTCCTAGTGAAGGGCAAGTGATTAGGATGTCCCATAAAAAATTTCATGCAGA TGCAATTCTTTCTTTTGCTAAACAAAACCAGGAGTCAGCAGTTTCCCAGCAAGCAGTCTATCATTCAGAG GACTTGGAAAACAGTGTGGGTGAACTTAGTGAAGGACAAAGACCCCAGCTAACAGCGGCAGCAGAGAACA TCTTAATGGGACATTCTCTCTATATGCAGCCACCTGTCACTAATACACAGTCTTTGGATCAACAATGTGA TCCTAAACCATTATCTCGGCAATTTGACACAGTTTCAGGTAGTATTTATGAAGATTCATGTGCTAGTCAT GGTCCAATGAGTTTGGGAGAATTGGAGTTGGAGCCAAATTCTAAGCTGGTTCTTCCCACAACACTTCTGA CAGCACAAGAAAATGATGTTAATTTACCAGTAGCCGCTGAAGATTTTTCCCAGTACCAACTAAAGCAAAA TCAGGATGTTAAGCAAGTTGAACACAAACCATCACAAAGTTACCTACGTGTTAGAAATAAATCTGATATT GCACCTTCACAGCAACAAGTTTTGATCAGCAGGATAAAGAATTGTAAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235274 representing NM_001244193 Red=Cloning site Green=Tags(s) MPVFKEVKVHLLEDAGIEKDAVTQETRISPSGIDSATTVAAATAAAIATAAPLIKVQSDLEAKVNSVTEL LSKLQETDKHLQRVTEQQTSIQRKQEKLHCHDHEKQMNVFMEQHIRHLEKLQQQQIDIQTHFISAALKTS SFQPVSMPSSRAVEKYSVKPEHPNLGSCNPSLYNTFASKQAPLKEVEDTSFDKQKSPLETPAPRRFAPVP VSRDDELSKRENLLEEKENMEVSCHRGNVRLLEQILNNNDSLTRKSESSNTTSLTRSKIGWTPEKTNRFP SCEELETTKVTMQKSDDVLHDLGQKEKETNSMVQPKESLSMLKLPDLPQNSVKLQTTNTTRSVLKDAEKI LRGVQNNKKVLEENLEAIIRAKDGAAMYSLINALSTNREMSEKIRIRKTVDEWIKTISAEIQDELSRTDY EQKRFDQKNQRTKKGQNMTKDIRTNTQDKTVNKSVIPRKHSQKQIEEHFRNLPMRGMPASSLQKERKEGL LKATTVIQDEDYMLQVYGKPVYQGHRSTLKKGPYLRFNSPSPKSRPQRPKVIERVKGTKVKSIRTQTDFY ATKPKKMDSKMKHSVPVLPHGDQQYLFSPSREMPTFSGTLEGHLIPMAILLGQTQSNSDTMPPAGVIVSK PHPVTVTTSIPPSSRKVETGVKKPNIAIVEMKSEKKDPPQLTVQVLPSVDIDSISNSSADVLSPLSSPKE ASLPPVQTWIKTPEIMKVDEEEVKFPGTNFDEIIDVIQEEEKCDEIPDSEPILEFNRSVKADSTKYNGPP FPPVASTFQPTADILDKVIERKETLENSLIQWVEQEIMSRIISGLFPVQQQIAPSISVSVSETSEPLTSD IVEGTSSGALQLFVDAGVPVNSNVIKHFVNEALAETIAVMLGDREAKKQGPVATGVSGDASTNETYLPAR VCTPLPTPQPTPPCSPSSPAKECVLVKTPDSSPCDSDHDMAFPVKEICAEKGDDMPAIMLVNTPTVTPTT TPPPAAAVFTPTLSDISIDKLKVSSPELPKPWGDGDLPLEEENPNSPQEELHPRAIVMSVAKDEEPESMD FPAQPPPPEPVPFMPFPAGTKAPSPSQMPGSDSSTLESTLSVTVTETETLDKPISEGEILFSCGQKLAPK ILEDIGLYLTNLNDSLSSTLHDAVEMEDDPPSEGQVIRMSHKKFHADAILSFAKQNQESAVSQQAVYHSE DLENSVGELSEGQRPQLTAAAENILMGHSLYMQPPVTNTQSLDQQCDPKPLSRQFDTVSGSIYEDSCASH GPMSLGELELEPNSKLVLPTTLLTAQENDVNLPVAAEDFSQYQLKQNQDVKQVEHKPSQSYLRVRNKSDI APSQQQVLISRIKNCKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001244193
ORF Size	4041 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244193.1, NP_001231122.1
RefSeq Size	4331 bp
RefSeq ORF	4044 bp
Locus ID	9786
UniProt ID	Q9BVV6
MW	149.6 kDa
Gene Summary	This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016]

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