KIAA0586 (NM_001244192) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JBTS23; SRTD14; Talpid3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332069 representing NM_001244192. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGTCAGAAAGTGATTTTTCTAAAGACGTTGCAGTGCAAGTGTTGCCTTTGGATAAAATAGAAGAG AACAACAAGCAAAAAGCAAATGACATCTTCATTTCTCAGTATACAATGGGACAGAAAGATGCTCTAAGA ACAGTTTTAAAGCAAAATGTCTCACTTTGTCTCACAGGCTGGAGTGATCACAGTGGGGTGATCACAACT CACTGCAGTCTCTACCTCCTCAGGCTCATGAGATCCTCCCACCTCAGCCTCCCGAGTAGCTGGGACTAC AGAGCTCAAAGCATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGATGCAGGCATAGAGAAG GATGCTGTTACTCAGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACAACCGTGGCTGCAGCA ACTGCTGCTGCCATTGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTGGAAGCAAAAGTCAAT TCTGTTACAGAATTACTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGTGTTACAGAGCAGCAA ACAAGCATTCAGAGGAAACAAGAGAAATTACATTGTCATGATCACGAAAAGCAAATGAATGTGTTTATG GAGCAGCACATAAGGCATCTTGAAAAGTTACAACAACAACAAATAGATATTCAGACTCATTTTATTAGT GCTGCACTCAAGACTAGTAGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCAGTGGAAAAGTATTCC GTAAAACCAGAACACCCTAATCTTGGTAGCTGTAATCCATCTTTATATAACACATTTGCTTCCAAACAA GCACCTTTAAAAGAAGTTGAAGATACGAGTTTTGATAAACAGAAATCTCCTTTGGAGACACCAGCACCT CGCAGATTTGCTCCTGTACCTGTTTCAAGGGATGATGAACTATCAAAGAGGGAAAATCTTTTGGAAGAA AAAGAAAATATGGAAGTGTCGTGTCACAGAGGAAATGTAAGACTATTGGAACAAATTTTGAATAATAAT GATTCTTTGACAAGAAAAAGTGAATCATCAAACACCACCTCACTAACTAGGTCAAAAATAGGATGGACT CCTGAGAAAACAAACAGATTTCCTTCCTGTGAAGAGCTAGAAACAACTAAAGTGACTATGCAGAAGTCT GATGATGTTCTTCATGACCTTGGCCAAAAAGAGAAAGAAACAAATAGCATGGTCCAGCCAAAAGAATCT CTGAGTATGTTGAAGCTTCCAGATCTTCCACAGAATTCTGTTAAGCTTCAAACAACCAATACAACAAGA TCTGTATTGAAAGATGCTGAGAAGATTTTGAGAGGAGTACAAAACAATAAAAAAGTACTTGAAGAAAAC CTGGAAGCTATTATTCGTGCAAAAGATGGAGCTGCCATGTATTCGCTTATCAATGCTTTATCTACCAAC AGAGAGATGTCAGAGAAAATTAGGATCAGAAAGACAGTGGATGAATGGATTAAAACTATTTCTGCAGAA ATTCAGGATGAACTGTCAAGAACAGATTATGAACAAAAAAGATTTGATCAGAAGAATCAGAGAACCAAG AAAGGTCAGAATATGACTAAAGATATTAGAACCAACACACAAGATAAAACTGTCAACAAATCTGTAATT CCAAGAAAACATTCTCAAAAGCAAATAGAAGAGCATTTTAGAAATCTACCTATGAGGGGCATGCCTGCT TCAAGTTTACAGAAAGAGAGAAAGGAAGGGCTTTTGAAAGCAACCACAGTAATACAAGATGAAGATTAT ATGTTACAAGTCTATGGAAAGCCAGTTTATCAGGGCCATCGAAGCACTCTTAAAAAAGGACCATATCTC AGATTTAATTCTCCATCTCCTAAGTCCAGACCACAGAGACCAAAAGTAATAGAACGAGTTAAAGGCACT AAGGTAAAGTCAATAAGAACACAGACTGACTTCTATGCAACAAAACCTAAGAAGATGGATTCTAAAATG AAACATTCTGTTCCTGTGTTACCTCATGGCGATCAGCAATATTTGTTCAGCCCAAGTAGAGAAATGCCT ACTTTTTCAGGTACATTGGAAGGTCATCTGATTCCTATGGCAATTCTTTTAGGACAAACCCAAAGTAAT AGTGATACCATGCCACCTGCTGGAGTGATTGTCAGCAAGCCACACCCTGTAACTGTGACTACTTCTATT CCTCCATCATCTCGAAAAGTAGAAACTGGAGTAAAGAAACCTAACATAGCCATTGTAGAAATGAAGTCA GAAAAAAAGGATCCTCCTCAGCTTACTGTGCAGGTATTACCCAGTGTAGATATTGACAGCATTTCAAAT AGTAGTGCTGATGTCCTTTCACCTCTGTCTAGCCCCAAAGAAGCATCTCTTCCTCCTGTGCAAACTTGG ATAAAGACTCCAGAAATTATGAAGGTAGATGAAGAAGAGGTGAAGTTTCCAGGAACTAACTTTGATGAA ATAATCGATGTCATACAGGAAGAAGAAAAATGTGATGAAATTCCAGACTCTGAACCAATTCTGGAGTTT AACAGAAGTGTTAAAGCTGATTCTACAAAATATAATGGTCCTCCATTTCCGCCAGTTGCTTCTACTTTT CAGCCCACTGCTGATATTCTGGATAAAGTAATTGAGAGAAAAGAAACACTGGAAAATAGCTTAATTCAA TGGGTAGAGCAAGAAATAATGTCAAGAATTATCTCTGGGCTCTTTCCAGTCCAGCAACAGATTGCACCT AGTATCAGTGTTTCAGTCAGTGAGACAAGTGAACCACTGACTTCTGACATTGTGGAAGGAACAAGCAGT GGCGCCCTCCAGCTTTTTGTTGATGCTGGTGTTCCTGTGAACTCAAATGTGATTAAACATTTTGTTAAC GAAGCTCTTGCTGAGACCATTGCTGTCATGCTGGGTGACAGAGAAGCAAAGAAGCAAGGTCCTGTTGCT ACAGGTGTTTCTGGGGATGCTTCAACAAATGAAACATATTTGCCGGCAAGAGTGTGCACCCCACTGCCT ACCCCACAGCCTACGCCTCCTTGCTCACCTTCATCACCTGCTAAGGAGTGTGTTTTGGTAAAGACTCCA GATTCTTCTCCCTGTGATTCGGATCATGATATGGCTTTTCCTGTGAAAGAAATATGTGCTGAAAAAGGA GATGATATGCCTGCCATCATGCTTGTTAATACTCCAACAGTTACCCCTACTACTACACCTCCTCCAGCG GCGGCAGTTTTTACCCCAACTTTGTCAGATATTTCCATTGATAAATTGAAGGTATCAAGCCCAGAGCTT CCCAAGCCATGGGGTGATGGAGACCTGCCACTGGAAGAAGAGAACCCTAACTCACCTCAAGAAGAACTT CATCCAAGAGCTATTGTAATGTCTGTGGCTAAGGATGAAGAACCAGAGAGTATGGATTTCCCTGCTCAG CCTCCACCTCCAGAGCCAGTTCCCTTTATGCCATTTCCTGCCGGCACCAAGGCCCCTTCCCCCTCACAG ATGCCAGGTTCTGATTCATCAACACTGGAGAGCACATTGAGTGTTACTGTCACTGAAACTGAAACTTTA GATAAACCCATCTCTGAAGGAGAGATTTTATTTAGCTGTGGTCAAAAATTGGCCCCCAAGATTTTAGAA GATATAGGACTGTACCTGACAAACCTTAATGATAGCTTATCCAGCACTCTGCATGATGCCGTTGAAATG GAGGATGATCCTCCTAGTGAAGGGCAAGTGATTAGGATGTCCCATAAAAAATTTCATGCAGATGCAATT CTTTCTTTTGCTAAACAAAACCAGGAGTCAGCAGTTTCCCAGCAAGCAGTCTATCATTCAGAGGACTTG GAAAACAGTGTGGGTGAACTTAGTGAAGGACAAAGACCCCAGCTAACAGCGGCAGCAGAGAACATCTTA ATGGGACATTCTCTCTATATGCAGCCACCTGTCACTAATACACAGTCTTTGGATCAACAATGTGATCCT AAACCATTATCTCGGCAATTTGACACAGTTTCAGGTAGTATTTATGAAGATTCATGTGCTAGTCATGGT CCAATGAGTTTGGGAGAATTGGAGTTGGAGCCAAATTCTAAGCTGGTTCTTCCCACAACACTTCTGACA GCACAAGAAAATGATGTTAATTTACCAGTAGCCGCTGAAGATTTTTCCCAGTACCAACTAAAGCAAAAT CAGGATGTTAAGCAAGTTGAACACAAACCATCACAAAGTTACCTACGTGTTAGAAATAAATCTGATATT GCACCTTCACAGCAACAAGTTTCACCAGGTGATATGGATCGGACACAAATTGAGCTTAATCCGTACCTC ACATGTGTATTTTCAGGTGGGAAAGCAGTGCCACTCTCCGCTTCACAGATGCCCCCTGCCAAGATGTCA GTGATGCTGCCGTCAGTGAACCTCGAGGACTGCTCTCAGTCTCTGAGTCTCAGCACAATGCAGGAGGAC ATGGAGTCTTCGGGGGCAGATACCTTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001244192
Insert Size	4515 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244192.1
RefSeq Size	5732 bp
RefSeq ORF	4515 bp
Locus ID	9786
UniProt ID	Q9BVV6
MW	166.4 kDa
Gene Summary	This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (4) lacks an alternate segment in the 5' coding region, uses a downstream start codon, and lacks an alternate in-frame 3' exon, compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1.

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