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KIAA0586 (NM_001244192) Human Tagged ORF Clone
CAT＃: RG235315
- TrueORF®
KIAA0586 (tGFP-tagged) - Homo sapiens KIAA0586 (KIAA0586), transcript variant 4
ORF Plasmid: DDK
"NM_001244192" in other vectors (2)
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CNY 16,530.00
货期*
14周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | JBTS23; SRTD14; Talpid3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235315 representing NM_001244192
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCAGAAAGTGATTTTTCTAAAGACGTTGCAGTGCAAGTGTTGCCTTTGGATAAAATAGAAGAGA ACAACAAGCAAAAAGCAAATGACATCTTCATTTCTCAGTATACAATGGGACAGAAAGATGCTCTAAGAAC AGTTTTAAAGCAAAATGTCTCACTTTGTCTCACAGGCTGGAGTGATCACAGTGGGGTGATCACAACTCAC TGCAGTCTCTACCTCCTCAGGCTCATGAGATCCTCCCACCTCAGCCTCCCGAGTAGCTGGGACTACAGAG CTCAAAGCATGCCTGTTTTTAAGGAAGTAAAGGTACATCTGTTAGAAGATGCAGGCATAGAGAAGGATGC TGTTACTCAGGAGACTAGAATTTCACCCAGTGGAATTGATTCAGCTACAACCGTGGCTGCAGCAACTGCT GCTGCCATTGCAACCGCAGCTCCGTTGATAAAGGTGCAGAGTGATTTGGAAGCAAAAGTCAATTCTGTTA CAGAATTACTTAGTAAATTACAGGAGACTGATAAACACCTGCAACGTGTTACAGAGCAGCAAACAAGCAT TCAGAGGAAACAAGAGAAATTACATTGTCATGATCACGAAAAGCAAATGAATGTGTTTATGGAGCAGCAC ATAAGGCATCTTGAAAAGTTACAACAACAACAAATAGATATTCAGACTCATTTTATTAGTGCTGCACTCA AGACTAGTAGTTTTCAGCCTGTTAGTATGCCCTCCTCCAGAGCAGTGGAAAAGTATTCCGTAAAACCAGA ACACCCTAATCTTGGTAGCTGTAATCCATCTTTATATAACACATTTGCTTCCAAACAAGCACCTTTAAAA GAAGTTGAAGATACGAGTTTTGATAAACAGAAATCTCCTTTGGAGACACCAGCACCTCGCAGATTTGCTC CTGTACCTGTTTCAAGGGATGATGAACTATCAAAGAGGGAAAATCTTTTGGAAGAAAAAGAAAATATGGA AGTGTCGTGTCACAGAGGAAATGTAAGACTATTGGAACAAATTTTGAATAATAATGATTCTTTGACAAGA AAAAGTGAATCATCAAACACCACCTCACTAACTAGGTCAAAAATAGGATGGACTCCTGAGAAAACAAACA GATTTCCTTCCTGTGAAGAGCTAGAAACAACTAAAGTGACTATGCAGAAGTCTGATGATGTTCTTCATGA CCTTGGCCAAAAAGAGAAAGAAACAAATAGCATGGTCCAGCCAAAAGAATCTCTGAGTATGTTGAAGCTT CCAGATCTTCCACAGAATTCTGTTAAGCTTCAAACAACCAATACAACAAGATCTGTATTGAAAGATGCTG AGAAGATTTTGAGAGGAGTACAAAACAATAAAAAAGTACTTGAAGAAAACCTGGAAGCTATTATTCGTGC AAAAGATGGAGCTGCCATGTATTCGCTTATCAATGCTTTATCTACCAACAGAGAGATGTCAGAGAAAATT AGGATCAGAAAGACAGTGGATGAATGGATTAAAACTATTTCTGCAGAAATTCAGGATGAACTGTCAAGAA CAGATTATGAACAAAAAAGATTTGATCAGAAGAATCAGAGAACCAAGAAAGGTCAGAATATGACTAAAGA TATTAGAACCAACACACAAGATAAAACTGTCAACAAATCTGTAATTCCAAGAAAACATTCTCAAAAGCAA ATAGAAGAGCATTTTAGAAATCTACCTATGAGGGGCATGCCTGCTTCAAGTTTACAGAAAGAGAGAAAGG AAGGGCTTTTGAAAGCAACCACAGTAATACAAGATGAAGATTATATGTTACAAGTCTATGGAAAGCCAGT TTATCAGGGCCATCGAAGCACTCTTAAAAAAGGACCATATCTCAGATTTAATTCTCCATCTCCTAAGTCC AGACCACAGAGACCAAAAGTAATAGAACGAGTTAAAGGCACTAAGGTAAAGTCAATAAGAACACAGACTG ACTTCTATGCAACAAAACCTAAGAAGATGGATTCTAAAATGAAACATTCTGTTCCTGTGTTACCTCATGG CGATCAGCAATATTTGTTCAGCCCAAGTAGAGAAATGCCTACTTTTTCAGGTACATTGGAAGGTCATCTG ATTCCTATGGCAATTCTTTTAGGACAAACCCAAAGTAATAGTGATACCATGCCACCTGCTGGAGTGATTG TCAGCAAGCCACACCCTGTAACTGTGACTACTTCTATTCCTCCATCATCTCGAAAAGTAGAAACTGGAGT AAAGAAACCTAACATAGCCATTGTAGAAATGAAGTCAGAAAAAAAGGATCCTCCTCAGCTTACTGTGCAG GTATTACCCAGTGTAGATATTGACAGCATTTCAAATAGTAGTGCTGATGTCCTTTCACCTCTGTCTAGCC CCAAAGAAGCATCTCTTCCTCCTGTGCAAACTTGGATAAAGACTCCAGAAATTATGAAGGTAGATGAAGA AGAGGTGAAGTTTCCAGGAACTAACTTTGATGAAATAATCGATGTCATACAGGAAGAAGAAAAATGTGAT GAAATTCCAGACTCTGAACCAATTCTGGAGTTTAACAGAAGTGTTAAAGCTGATTCTACAAAATATAATG GTCCTCCATTTCCGCCAGTTGCTTCTACTTTTCAGCCCACTGCTGATATTCTGGATAAAGTAATTGAGAG AAAAGAAACACTGGAAAATAGCTTAATTCAATGGGTAGAGCAAGAAATAATGTCAAGAATTATCTCTGGG CTCTTTCCAGTCCAGCAACAGATTGCACCTAGTATCAGTGTTTCAGTCAGTGAGACAAGTGAACCACTGA CTTCTGACATTGTGGAAGGAACAAGCAGTGGCGCCCTCCAGCTTTTTGTTGATGCTGGTGTTCCTGTGAA CTCAAATGTGATTAAACATTTTGTTAACGAAGCTCTTGCTGAGACCATTGCTGTCATGCTGGGTGACAGA GAAGCAAAGAAGCAAGGTCCTGTTGCTACAGGTGTTTCTGGGGATGCTTCAACAAATGAAACATATTTGC CGGCAAGAGTGTGCACCCCACTGCCTACCCCACAGCCTACGCCTCCTTGCTCACCTTCATCACCTGCTAA GGAGTGTGTTTTGGTAAAGACTCCAGATTCTTCTCCCTGTGATTCGGATCATGATATGGCTTTTCCTGTG AAAGAAATATGTGCTGAAAAAGGAGATGATATGCCTGCCATCATGCTTGTTAATACTCCAACAGTTACCC CTACTACTACACCTCCTCCAGCGGCGGCAGTTTTTACCCCAACTTTGTCAGATATTTCCATTGATAAATT GAAGGTATCAAGCCCAGAGCTTCCCAAGCCATGGGGTGATGGAGACCTGCCACTGGAAGAAGAGAACCCT AACTCACCTCAAGAAGAACTTCATCCAAGAGCTATTGTAATGTCTGTGGCTAAGGATGAAGAACCAGAGA GTATGGATTTCCCTGCTCAGCCTCCACCTCCAGAGCCAGTTCCCTTTATGCCATTTCCTGCCGGCACCAA GGCCCCTTCCCCCTCACAGATGCCAGGTTCTGATTCATCAACACTGGAGAGCACATTGAGTGTTACTGTC ACTGAAACTGAAACTTTAGATAAACCCATCTCTGAAGGAGAGATTTTATTTAGCTGTGGTCAAAAATTGG CCCCCAAGATTTTAGAAGATATAGGACTGTACCTGACAAACCTTAATGATAGCTTATCCAGCACTCTGCA TGATGCCGTTGAAATGGAGGATGATCCTCCTAGTGAAGGGCAAGTGATTAGGATGTCCCATAAAAAATTT CATGCAGATGCAATTCTTTCTTTTGCTAAACAAAACCAGGAGTCAGCAGTTTCCCAGCAAGCAGTCTATC ATTCAGAGGACTTGGAAAACAGTGTGGGTGAACTTAGTGAAGGACAAAGACCCCAGCTAACAGCGGCAGC AGAGAACATCTTAATGGGACATTCTCTCTATATGCAGCCACCTGTCACTAATACACAGTCTTTGGATCAA CAATGTGATCCTAAACCATTATCTCGGCAATTTGACACAGTTTCAGGTAGTATTTATGAAGATTCATGTG CTAGTCATGGTCCAATGAGTTTGGGAGAATTGGAGTTGGAGCCAAATTCTAAGCTGGTTCTTCCCACAAC ACTTCTGACAGCACAAGAAAATGATGTTAATTTACCAGTAGCCGCTGAAGATTTTTCCCAGTACCAACTA AAGCAAAATCAGGATGTTAAGCAAGTTGAACACAAACCATCACAAAGTTACCTACGTGTTAGAAATAAAT CTGATATTGCACCTTCACAGCAACAAGTTTCACCAGGTGATATGGATCGGACACAAATTGAGCTTAATCC GTACCTCACATGTGTATTTTCAGGTGGGAAAGCAGTGCCACTCTCCGCTTCACAGATGCCCCCTGCCAAG ATGTCAGTGATGCTGCCGTCAGTGAACCTCGAGGACTGCTCTCAGTCTCTGAGTCTCAGCACAATGCAGG AGGACATGGAGTCTTCGGGGGCAGATACCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235315 representing NM_001244192
Red=Cloning site Green=Tags(s) MVSESDFSKDVAVQVLPLDKIEENNKQKANDIFISQYTMGQKDALRTVLKQNVSLCLTGWSDHSGVITTH CSLYLLRLMRSSHLSLPSSWDYRAQSMPVFKEVKVHLLEDAGIEKDAVTQETRISPSGIDSATTVAAATA AAIATAAPLIKVQSDLEAKVNSVTELLSKLQETDKHLQRVTEQQTSIQRKQEKLHCHDHEKQMNVFMEQH IRHLEKLQQQQIDIQTHFISAALKTSSFQPVSMPSSRAVEKYSVKPEHPNLGSCNPSLYNTFASKQAPLK EVEDTSFDKQKSPLETPAPRRFAPVPVSRDDELSKRENLLEEKENMEVSCHRGNVRLLEQILNNNDSLTR KSESSNTTSLTRSKIGWTPEKTNRFPSCEELETTKVTMQKSDDVLHDLGQKEKETNSMVQPKESLSMLKL PDLPQNSVKLQTTNTTRSVLKDAEKILRGVQNNKKVLEENLEAIIRAKDGAAMYSLINALSTNREMSEKI RIRKTVDEWIKTISAEIQDELSRTDYEQKRFDQKNQRTKKGQNMTKDIRTNTQDKTVNKSVIPRKHSQKQ IEEHFRNLPMRGMPASSLQKERKEGLLKATTVIQDEDYMLQVYGKPVYQGHRSTLKKGPYLRFNSPSPKS RPQRPKVIERVKGTKVKSIRTQTDFYATKPKKMDSKMKHSVPVLPHGDQQYLFSPSREMPTFSGTLEGHL IPMAILLGQTQSNSDTMPPAGVIVSKPHPVTVTTSIPPSSRKVETGVKKPNIAIVEMKSEKKDPPQLTVQ VLPSVDIDSISNSSADVLSPLSSPKEASLPPVQTWIKTPEIMKVDEEEVKFPGTNFDEIIDVIQEEEKCD EIPDSEPILEFNRSVKADSTKYNGPPFPPVASTFQPTADILDKVIERKETLENSLIQWVEQEIMSRIISG LFPVQQQIAPSISVSVSETSEPLTSDIVEGTSSGALQLFVDAGVPVNSNVIKHFVNEALAETIAVMLGDR EAKKQGPVATGVSGDASTNETYLPARVCTPLPTPQPTPPCSPSSPAKECVLVKTPDSSPCDSDHDMAFPV KEICAEKGDDMPAIMLVNTPTVTPTTTPPPAAAVFTPTLSDISIDKLKVSSPELPKPWGDGDLPLEEENP NSPQEELHPRAIVMSVAKDEEPESMDFPAQPPPPEPVPFMPFPAGTKAPSPSQMPGSDSSTLESTLSVTV TETETLDKPISEGEILFSCGQKLAPKILEDIGLYLTNLNDSLSSTLHDAVEMEDDPPSEGQVIRMSHKKF HADAILSFAKQNQESAVSQQAVYHSEDLENSVGELSEGQRPQLTAAAENILMGHSLYMQPPVTNTQSLDQ QCDPKPLSRQFDTVSGSIYEDSCASHGPMSLGELELEPNSKLVLPTTLLTAQENDVNLPVAAEDFSQYQL KQNQDVKQVEHKPSQSYLRVRNKSDIAPSQQQVSPGDMDRTQIELNPYLTCVFSGGKAVPLSASQMPPAK MSVMLPSVNLEDCSQSLSLSTMQEDMESSGADTF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001244192 |
| ORF Size | 4512 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001244192.2 |
| RefSeq Size | 5732 bp |
| RefSeq ORF | 4515 bp |
| Locus ID | 9786 |
| UniProt ID | Q9BVV6 |
| Gene Summary | This gene encodes a conserved centrosomal protein that functions in ciliogenesis and responds to hedgehog signaling. Mutations in this gene causes Joubert syndrome 23. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Aug 2016] |
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