PRPSAP2 (NM_001243940) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PAP41
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332055 representing NM_001243940. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTTTGTGTGACGCCACCTGAATTAGAAACCAAGATGAACATAACCAAAGGTGGTCTGGTGTTGTTT TCAGCAAACTCGAATTCATCATGTATGGAGCTATCAAAGAAAATTGCAGAGCGGCTAGGGGTGGAGATG GGCAAAGTGCAGGTTTACCAGGAACCTAACAGAGAAACAAGAGTACAAATTCAAGAGTCTGTGAGGGGA AAAGATGTTTTCATCATCCAAACTGTTTCGAAGGACGTGAACACCACCATCATGGAGCTCCTGATCATG GTGTATGCATGTAAGACCTCTTGTGCCAAGAGCATCATTGGCGTGATACCCTACTTTCCTTACAGCAAG CAGTGCAAGATGAGAAAAAGAGGCTCCATTGTCTCTAAATTGCTGGCTTCCATGATGTGCAAAGCTGGT CTAACTCATCTTATTACTATGGATTTACACCAGAAGGAAATTCAGGGCTTCTTCAATATTCCTGTTGAC AATTTAAGAGCATCTCCCTTCTTATTACAGTATATTCAAGAAGAGATCCCAGATTACAGGAATGCAGTA ATCGTGGCCAAGTCTCCAGCCTCGGCGAAGAGGGCACAGTCTTTTGCTGAGCGCCTGCGCCTGGGAATT GCAGTGATTCATGGAGAGGCGCAGGATGCCGAGTCGGACTTGGTGGATGGACGGCATTCCCCACCCATG GTCAGAAGTGTGGCTGCCATCCACCCCAGCCTGGAGATCCCCATGCTGATTCCTAAAGAAAAGCCCCCA ATCACGGTTGTGGGTGATGTTGGAGGAAGGATTGCCATCATCGTGGTGGTGGTCACCAATACAATTCCA CATGAAGTCCAGAAGCTCCAGTGCCCCAAGATTAAAACTGTGGATATCAGCATGATCCTTTCAGAGGCG ATCCGTCGGATCCACAATGGGGAGTCCATGTCCTACCTTTTCAGAAACATAGGCTTAGATGACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243940
Insert Size	963 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243940.1
RefSeq Size	1680 bp
RefSeq ORF	963 bp
Locus ID	5636
UniProt ID	O60256
Protein Families	Druggable Genome
MW	35.5 kDa
Gene Summary	This gene encodes a protein that associates with the enzyme phosphoribosylpyrophosphate synthetase (PRS). PRS catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] Transcript Variant: This variant (3) has a shorter 5' UTR and lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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