CACNA1B (NM_001243812) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BIII; CACNL1A5; CACNN; Cav2.2; DYT23; NEDNEH
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332047 representing NM_001243812. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTCCGCTTCGGGGACGAGCTGGGCGGCCGCTATGGGGGCCCCGGCGGCGGAGAGCGGGCCCGGGGC GGCGGGGCCGGCGGGGCGGGGGGCCCGGGTCCCGGGGGGCTGCAGCCCGGCCAGCGGGTCCTCTACAAG CAATCGATCGCGCAGCGCGCGCGGACCATGGCGCTGTACAACCCCATCCCGGTCAAGCAGAACTGCTTC ACCGTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCGAAGCGCATCACC GAGTGGCCTCCATTCGAGTATATGATCCTGGCCACCATCATCGCCAACTGCATCGTGCTGGCCCTGGAG CAGCACCTCCCTGATGGGGACAAAACGCCCATGTCCGAGCGGCTGGACGACACGGAGCCCTATTTCATC GGGATCTTTTGCTTCGAGGCAGGGATCAAAATCATCGCTCTGGGCTTTGTCTTCCACAAGGGCTCTTAC CTGCGGAACGGCTGGAACGTCATGGACTTCGTGGTCGTCCTCACAGGGATCCTTGCCACGGCTGGAACT GACTTCGACCTGCGAACACTGAGGGCTGTGCGTGTGCTGAGGCCCCTGAAGCTGGTGTCTGGGATTCCA AGTTTGCAGGTGGTGCTCAAGTCCATCATGAAGGCCATGGTTCCACTCCTGCAGATTGGGCTGCTTCTC TTCTTTGCCATCCTCATGTTTGCCATCATTGGCCTGGAGTTCTACATGGGCAAGTTCCACAAGGCCTGT TTCCCCAACAGCACAGATGCGGAGCCCGTGGGTGACTTCCCCTGTGGCAAGGAGGCCCCAGCCCGGCTG TGCGAGGGCGACACTGAGTGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAACTTTGACAAT ATCCTGTTTGCCATCTTGACGGTGTTCCAGTGCATCACCATGGAGGGCTGGACTGACATCCTCTATAAT ACAAACGATGCGGCCGGCAACACCTGGAACTGGCTCTACTTCATCCCTCTCATCATCATCGGCTCCTTC TTCATGCTCAACCTGGTGCTGGGCGTGCTCTCGGGGGAGTTTGCCAAGGAGCGAGAGAGGGTGGAGAAC CGCCGCGCCTTCCTGAAGCTGCGCCGGCAGCAGCAGATCGAGCGAGAGCTCAACGGGTACCTGGAGTGG ATCTTCAAGGCGGAGGAAGTCATGCTGGCCGAGGAGGACAGGAATGCAGAGGAGAAGTCCCCTTTGGAC GTGCTGAAGAGAGCGGCCACCAAGAAGAGCAGAAATGACCTGATCCACGCAGAGGAGGGAGAGGACCGG TTTGCAGATCTCTGTGCTGTTGGATCCCCCTTCGCCCGCGCCAGCCTCAAGAGCGGGAAGACAGAGAGC TCGTCATACTTCCGGAGGAAGGAGAAGATGTTCCGGTTTTTTATCCGGCGCATGGTGAAGGCTCAGAGC TTCTACTGGGTGGTGCTGTGCGTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCATTACAACCAG CCGCGGCGGCTTACCACGACCCTGTATTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATG TCCCTGAAGATGTATGGCCTGGGGCCCAGAAGCTACTTCCGGTCCTCCTTCAACTGCTTCGACTTTGGG GTCATCGTGGGGAGCGTCTTTGAAGTGGTCTGGGCGGCCATCAAGCCGGGAAGCTCCTTTGGGATCAGT GTGCTGCGGGCCCTCCGCCTGCTGAGGATCTTCAAAGTCACGAAGTACTGGAGCTCCCTGCGGAACCTG GTGGTGTCCCTGCTGAACTCCATGAAGTCCATCATCAGCCTGCTCTTCTTGCTCTTCCTGTTCATTGTG GTCTTCGCCCTGCTGGGGATGCAGCTGTTTGGGGGACAGTTCAACTTCCAGGATGAGACTCCCACAACC AACTTCGACACCTTCCCTGCCGCCATCCTCACTGTCTTCCAGATCCTGACGGGAGAGGACTGGAATGCA GTGATGTATCACGGGATCGAATCGCAAGGCGGCGTCAGCAAAGGCATGTTCTCGTCCTTTTACTTCATT GTCCTGACACTGTTCGGAAACTACACTCTGCTGAATGTCTTTCTGGCCATCGCTGTGGACAACCTGGCC AACGCCCAAGAGCTGACCAAGGATGAAGAGGAGATGGAAGAAGCAGCCAATCAGAAGCTTGCTCTGCAA AAGGCCAAAGAAGTGGCTGAAGTCAGCCCCATGTCTGCCGCGAACATCTCCATCGCCGCCAGGCAGCAG AACTCGGCCAAGGCGCGCTCGGTGTGGGAGCAGCGGGCCAGCCAGCTACGGCTGCAGAACCTGCGGGCC AGCTGCGAGGCGCTGTACAGCGAGATGGACCCCGAGGAGCGGCTGCGCTTCGCCACTACGCGCCACCTG CGGCCCGACATGAAGACGCACCTGGACCGGCCGCTGGTGGTGGAGCTGGGCCGCGACGGCGCGCGGGGG CCCGTGGGAGGCAAAGCCCGACCTGAGGCTGCGGAGGCCCCCGAGGGCGTCGACCCTCCGCGCAGGCAC CACCGGCACCGCGACAAGGACAAGACCCCCGCGGCGGGGGACCAGGACCGAGCAGAGGCCCCGAAGGCG GAGAGCGGGGAGCCCGGTGCCCGGGAGGAGCGGCCGCGGCCGCACCGCAGCCACAGCAAGGAGGCCGCG GGGCCCCCGGAGGCGCGGAGCGAGCGCGGCCGAGGCCCAGGCCCCGAGGGCGGCCGGCGGCACCACCGG CGCGGCTCCCCGGAGGAGGCGGCCGAGCGGGAGCCCCGACGCCACCGCGCGCACCGGCACCAGGATCCG AGCAAGGAGTGCGCCGGCGCCAAGGGCGAGCGGCGCGCGCGGCACCGCGGCGGCCCCCGAGCGGGGCCC CGGGAGGCGGAGAGCGGGGAGGAGCCGGCGCGGCGGCACCGGGCCCGGCACAAGGCGCAGCCTGCTCAC GAGGCTGTGGAGAAGGAGACCACGGAGAAGGAGGCCACGGAGAAGGAGGCTGAGATAGTGGAAGCCGAC AAGGAAAAGGAGCTCCGGAACCACCAGCCCCGGGAGCCACACTGTGACCTGGAGACCAGTGGGACTGTG ACTGTGGGTCCCATGCACACACTGCCCAGCACCTGTCTCCAGAAGGTGGAGGAACAGCCAGAGGATGCA GACAATCAGCGGAACGTCACTCGCATGGGCAGTCAGCCCCCAGACCCGAACACTATTGTACATATCCCA GTGATGCTGACGGGCCCTCTTGGGGAAGCCACGGTCGTTCCCAGTGGTAACGTGGACCTGGAAAGCCAA GCAGAGGGGAAGAAGGAGGTGGAAGCGGATGACGTGATGAGGAGCGGCCCCCGGCCTATCGTCCCATAC AGCTCCATGTTCTGTTTAAGCCCCACCAACCTGCTCCGCCGCTTCTGCCACTACATCGTGACCATGAGG TACTTCGAGGTGGTCATTCTCGTGGTCATCGCCTTGAGCAGCATCGCCCTGGCTGCTGAGGACCCAGTG CGCACAGACTCGCCCAGGAACAACGCTCTGAAATACCTGGATTACATTTTCACTGGTGTCTTTACCTTT GAGATGGTGATAAAGATGATCGACTTGGGACTGCTGCTTCACCCTGGAGCCTATTTCCGGGACTTGTGG AACATTCTGGACTTCATTGTGGTCAGTGGCGCCCTGGTGGCGTTTGCTTTCTCAGGATCCAAAGGGAAA GACATCAATACCATCAAGTCTCTGAGAGTCCTTCGTGTCCTGCGGCCCCTCAAGACCATCAAACGGCTG CCCAAGCTCAAGGCTGTGTTTGACTGTGTGGTGAACTCCCTGAAGAATGTCCTCAACATCTTGATTGTC TACATGCTCTTCATGTTCATATTTGCCGTCATTGCGGTGCAGCTCTTCAAAGGGAAGTTTTTCTACTGC ACAGATGAATCCAAGGAGCTGGAGAGGGACTGCAGGGGTCAGTATTTGGATTATGAGAAGGAGGAAGTG GAAGCTCAGCCCAGGCAGTGGAAGAAATACGACTTTCACTACGACAATGTGCTCTGGGCTCTGCTGACG CTGTTCACAGTGTCCACGGGAGAAGGCTGGCCCATGGTGCTGAAACACTCCGTGGATGCCACCTATGAG GAGCAGGGTCCAAGCCCTGGGTACCGCATGGAGCTGTCCATCTTCTACGTGGTCTACTTTGTGGTCTTT CCCTTCTTCTTCGTCAACATCTTTGTGGCTTTGATCATCATCACCTTCCAGGAGCAGGGGGACAAGGTG ATGTCTGAATGCAGCCTGGAGAAGAACGAGAGGGCTTGCATTGACTTCGCCATCAGCGCCAAACCCCTG ACACGGTACATGCCCCAAAACCGGCAGTCGTTCCAGTATAAGACGTGGACATTTGTGGTCTCCCCGCCC TTTGAATACTTCATCATGGCCATGATAGCCCTCAACACTGTGGTGCTGATGATGAAGTTCTATGATGCA CCCTATGAGTACGAGCTGATGCTGAAATGCCTGAACATCGTGTTCACATCCATGTTCTCCATGGAATGC GTGCTGAAGATCATCGCCTTTGGGGTGCTGAACTATTTCAGAGATGCCTGGAATGTCTTTGACTTTGTC ACTGTGTTGGGAAGTATTACTGATATTTTAGTAACAGAGATTGCGGAAACGAACAATTTCATCAACCTC AGCTTCCTCCGCCTCTTTCGAGCTGCGCGGCTGATCAAGCTGCTCCGCCAGGGCTACACCATCCGCATC CTGCTGTGGACCTTTGTCCAGTCCTTCAAGGCCCTGCCCTACGTGTGTCTGCTCATTGCCATGCTGTTC TTCATCTACGCCATCATCGGCATGCAGGTGTTTGGGAATATTGCCCTGGATGATGACACCAGCATCAAC CGCCACAACAACTTCCGGACGTTTTTGCAAGCCCTGATGCTGCTGTTCAGGAGCGCCACGGGGGAGGCC TGGCACGAGATCATGCTGTCCTGCCTGAGCAACCAGGCCTGTGATGAGCAGGCCAATGCCACCGAGTGT GGAAGTGACTTTGCCTACTTCTACTTCGTCTCCTTCATCTTCCTGTGCTCCTTTCTGATGTTGAACCTC TTTGTGGCTGTGATCATGGACAATTTTGAGTACCTCACGCGGGACTCTTCCATCCTAGGTCCTCACCAC TTGGATGAGTTCATCCGGGTCTGGGCTGAATACGACCCGGCTGCGTGTGGGCGCATCAGTTACAATGAC ATGTTTGAGATGCTGAAACACATGTCCCCGCCTCTGGGGCTGGGGAAGAAATGCCCTGCTCGAGTTGCT TACAAGCGCCTGGTTCGCATGAACATGCCCATCTCCAACGAGGACATGACTGTTCACTTCACGTCCACG CTGATGGCCCTCATCCGGACGGCACTGGAGATCAAGCTGGCCCCAGCTGGGACAAAGCAGCATCAGTGT GACGCGGAGTTGAGGAAGGAGATTTCCGTTGTGTGGGCCAATCTGCCCCAGAAGACTTTGGACTTGCTG GTACCACCCCATAAGCCTGATGAGATGACAGTGGGGAAGGTTTATGCAGCTCTGATGATATTCGACTTC TACAAGCAGAACAAAACCACCAGAGACCAGATGCAGCAGGCTCCTGGAGGCCTCTCCCAGATGGGTCCT GTGTCCCTGTTCCACCCTCTGAAGGCCACCCTGGAGCAGACACAGCCGGCTGTGCTCCGAGGAGCCCGG GTTTTCCTTCGACAGAAGAGTTCCACCTCCCTCAGCAATGGCGGGGCCATACAAAACCAAGAGAGTGGC ATCAAAGAGTCTGTCTCCTGGGGCACTCAAAGGACCCAGGATGCACCCCATGAGGCCAGGCCACCCCTG GAGCGTGGCCACTCCACAGAGATCCCTGTGGGGCGGTCAGGAGCACTGGCTGTGGACGTTCAGATGCAG AGCATAACCCGGAGGGGCCCTGATGGGGAGCCCCAGCCTGGGCTGGAGAGCCAGGGTCGAGCGGCCTCC ATGCCCCGCCTTGCGGCCGAGACTCAGCCCGTCACAGATGCCAGCCCCATGAAGCGCTCCATCTCCACG CTGGCCCAGCGGCCCCGTGGGACTCATCTTTGCAGCACCACCCCGGACCGCCCACCCCCTAGCCAGGCG TCGTCGCACCACCACCACCACCGCTGCCACCGCCGCAGGGACAGGAAGCAGAGGTCCCTGGAGAAGGGG CCCAGCCTGTCTGCCGATATGGATGGCGCACCAAGCAGTGCTGTGGGGCCGGGGCTGCCCCCGGGAGAG GGGCCTACAGGCTGCCGGCGGGAACGAGAGCGCCGGCAGGAGCGGGGCCGGTCCCAGGAGCGGAGGCAG CCCTCATCCTCCTCCTCGGAGAAGCAGCGCTTCTACTCCTGCGACCGCTTTGGGGGCCGTGAGCCCCCG AAGCCCAAGCCCTCCCTCAGCAGCCACCCAACGTCGCCAACAGCTGGCCAGGAGCCGGGACCCCACCCA CAGGCCGGCTCAGCCGTGGGCTTTCCGAACACAACGCCCTGCTGCAGAGAGACCCCCTCAGCCAGCCCC TGGCCCCTGGCTCTCGAATTGGCTCTGACCCTTACCTGGGGCAGCGTCTGGACAGTGAGGCCTCTGTCC ACGCCCTGCCTGAGGACACTCTCACTTTCGAGGAGGCTGTGGCCACCAACTCGGGCCGCTCCTCCAGGA CTTCCTACGTGTCCTCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243812
Insert Size	6714 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243812.1
RefSeq Size	9618 bp
RefSeq ORF	6714 bp
Locus ID	774
UniProt ID	Q00975
Protein Families	Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways	Calcium signaling pathway, MAPK signaling pathway, Taste transduction, Type II diabetes mellitus
MW	251.7 kDa
Gene Summary	The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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