Nectin 3 (NECTIN3) (NM_001243286) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD113; CDW113; NECTIN-3; PPR3; PRR3; PVRL3; PVRR3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331988 representing NM_001243286. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGCGGACCCTGCGGCCGTCCCCGCTGTGTCCTGGAGGCGGCAAAGCACAACTTTCCTCCGCTTCT CTCCTCGGAGCCGGGCTCCTGCTGCAGCCCCCGACGCCACCTCCGCTGCTGCTGCTGCTCTTCCCGCTG CTGCTCTTCTCCAGGCTCTGTGGTGCCTTAGCTGGACCAATTATTGTGGAGCCACATGTCACAGCAGTA TGGGGAAAGAATGTTTCATTAAAGTGTTTAATTGAAGTAAATGAAACCATAACACAGATTTCATGGGAG AAGATACATGGCAAAAGTTCACAGACTGTTGCAGTTCACCATCCCCAATATGGATTCTCTGTTCAAGGA GAATATCAGGGAAGAGTCTTGTTTAAAAATTACTCACTTAATGATGCAACAATTACTCTGCATAACATA GGATTCTCTGATTCTGGAAAATACATCTGCAAAGCTGTTACATTCCCGCTTGGAAATGCCCAGTCCTCT ACAACTGTAACTGTGTTAGTTGAACCCACTGTGAGCCTGATAAAAGGGCCAGATTCTTTAATTGATGGA GGAAATGAAACAGTAGCAGCCATTTGCATCGCAGCCACTGGAAAACCCGTTGCACATATTGACTGGGAA GGTGATCTTGGTGAAATGGAATCCACTACAACTTCTTTTCCAAATGAAACGGCAACGATTATCAGCCAG TACAAGCTATTTCCAACCAGATTTGCTAGAGGAAGGCGAATTACTTGTGTTGTAAAACATCCAGCCTTG GAAAAGGACATCCGATACTCTTTCATATTAGACATACAGTATGCTCCTGAAGTTTCGGTAACAGGATAT GATGGAAATTGGTTTGTAGGAAGAAAAGGTGTTAATCTCAAATGTAATGCTGATGCAAATCCACCACCC TTCAAATCTGTGTGGAGCAGGTTGGATGGACAATGGCCTGATGGTTTATTGGCTTCAGACAATACTCTT CATTTTGTCCATCCATTGACTTTCAATTATTCTGGTGTTTATATCTGTAAAGTGACCAATTCCCTTGGT CAAAGAAGTGACCAAAAAGTCATCTACATTTCAGCCTACAATTCAGTGGCATCCCTCAACTGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243286
Insert Size	1101 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243286.1
RefSeq Size	5239 bp
RefSeq ORF	1101 bp
Locus ID	25945
UniProt ID	Q9NQS3
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Adherens junction, Cell adhesion molecules (CAMs)
MW	39.7 kDa
Gene Summary	This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) uses an alternate splice site that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (2) has a distinct and shorter C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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