HIP1 (NM_001243198) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HIP-I; ILWEQ; SHON; SHONbeta; SHONgamma
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331969 representing NM_001243198. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATCGGATGGCCAGCTCCATGAAGCAGGTGCCCAACCCACTGCCCAAGGTGCTGAGCCGGCGCGGG GTCGGCGCTGGGCTGGAGGCGGCGGAGCGCGAGAGCTTCGAGCGGACTCAGACTGTCAGCATCAATAAG GCCATTAATACGCAGGAAGTGGCTGTAAAGGAAAAACACGCCAGAACGTGCATACTGGGCACCCACCAT GAGAAAGGGGCACAGACCTTCTGGTCTGTTGTCAACCGCCTGCCTCTGTCTAGCAACGCAGTGCTCTGC TGGAAGTTCTGCCATGTGTTCCACAAACTCCTCCGAGATGGACACCCGAACGTCCTGAAGGACTCTCTG AGATACAGAAATGAATTGAGTGACATGAGCAGGATGTGGGGCCACCTGAGCGAGGGGTATGGCCAGCTG TGCAGCATCTACCTGAAACTGCTAAGAACCAAGATGGAGTACCACACCAAAAATCCCAGGTTCCCAGGC AACCTGCAGATGAGTGACCGCCAGCTGGACGAGGCTGGAGAAAGTGACGTGAACAACTTTTTCCAGTTA ACAGTGGAGATGTTTGACTACCTGGAGTGTGAACTCAACCTCTTCCAAACAGTATTCAACTCCCTGGAC ATGTCCCGCTCTGTGTCCGTGACGGCAGCAGGGCAGTGCCGCCTCGCCCCGCTGATCCAGGTCATCTTG GACTGCAGCCACCTTTATGACTACACTGTCAAGCTTCTCTTCAAACTCCACTCCTGCCTCCCAGCTGAC ACCCTGCAAGGCCACCGGGACCGCTTCATGGAGCAGTTTACAAAGTTGAAAGATCTGTTCTACCGCTCC AGCAACCTGCAGTACTTCAAGCGGCTCATTCAGATCCCCCAGCTGCCTGAGAACCCACCCAACTTCCTG CGAGCCTCAGCCCTGTCAGAACATATCAGCCCTGTGGTGGTGATCCCTGCAGAGGCCTCATCCCCCGAC AGCGAGCCAGTCCTAGAGAAGGATGACCTCATGGACATGGATGCCTCTCAGCAGAATTTATTTGACAAC AAGTTTGATGACATCTTTGGCAGTTCATTCAGCAGTGATCCCTTCAATTTCAACAGTCAAAATGGTGTG AACAAGGATGAGAAGGACCACTTAATTGAGCGACTATACAGAGAGATCAGTGGATTGAAGGCACAGCTA GAAAACATGAAGACTGAGAGCCAGCGGGTTGTGCTGCAGCTGAAGGGCCACGTCAGCGAGCTGGAAGCA GATCTGGCCGAGCAGCAGCACCTGCGGCAGCAGGCGGCCGACGACTGTGAATTCCTGCGGGCAGAACTG GACGAGCTCAGGAGGCAGCGGGAGGACACCGAGAAGGCTCAGCGGAGCCTGTCTGAGATAGAAAGGAAA GCTCAAGCCAATGAACAGCGATATAGCAAGCTAAAGGAGAAGTACAGCGAGCTGGTTCAGAACCACGCT GACCTGCTGCGGAAGAATGCAGAGGTGACCAAACAGGTGTCCATGGCCAGACAAGCCCAGGTAGATTTG GAACGAGAGAAAAAAGAGCTGGAGGATTCGTTGGAGCGCATCAGTGACCAGGGCCAGCGGAAGACTCAA GAACAGCTGGAAGTTCTAGAGAGCTTGAAGCAGGAACTTGCCACAAGCCAACGGGAGCTTCAGGTTCTG CAAGGCAGCCTGGAAACTTCTGCCCAGTCAGAAGCAAACTGGGCAGCCGAGTTCGCCGAGCTAGAGAAG GAGCGGGACAGCCTGGTGAGTGGCGCAGCTCATAGGGAGGAGGAATTATCTGCTCTTCGGAAAGAACTG CAGGACACTCAGCTCAAACTGGCCAGCACAGAGGAATCTATGTGCCAGCTTGCCAAAGACCAACGAAAA ATGCTTCTGGTGGGGTCCAGGAAGGCTGCGGAGCAGGTGATACAAGACGCCCTGAACCAGCTTGAAGAA CCTCCTCTCATCAGCTGCGCTGGGTCTGCAGATCACCTCCTCTCCACGGTCACATCCATTTCCAGCTGC ATCGAGCAACTGGAGAAAAGCTGGAGCCAGTATCTGGCCTGCCCAGAAGACATCAGTGGACTTCTCCAT TCCATAACCCTGCTGGCCCACTTGACCAGCGACGCCATTGCTCATGGTGCCACCACCTGCCTCAGAGCC CCACCTGAGCCTGCCGACTCACTGACCGAGGCCTGTAAGCAGTATGGCAGGGAAACCCTCGCCTACCTG GCCTCCCTGGAGGAAGAGGGAAGCCTTGAGAATGCCGACAGCACAGCCATGAGGAACTGCCTGAGCAAG ATCAAGGCCATCGGCGAGGAGCTCCTGCCCAGGGGACTGGACATCAAGCAGGAGGAGCTGGGGGACCTG GTGGACAAGGAGATGGCGGCCACTTCAGCTGCTATTGAAACTGCCACGGCCAGAATAGAGGGTACAGCA TCCCCTAAAGAGTTTTATGCCAAGAACTCTCGATGGACAGAAGGACTTATCTCAGCCTCCAAGGCTGTG GGCTGGGGAGCCACTGTCATGGTGGATGCAGCTGATCTGGTGGTACAAGGCAGAGGGAAATTTGAGGAG CTAATGGTGTGTTCTCATGAAATTGCTGCTAGCACAGCCCAGCTTGTGGCTGCATCCAAGGTGAAAGCT GATAAGGACAGCCCCAACCTAGCCCAGCTGCAGCAGGCCTCTCGGGGAGTGAACCAGGCCACTGCCGGC GTTGTGGCCTCAACCATTTCCGGCAAATCACAGATCGAAGAGACAGACAACATGGACTTCTCAAGCATG ACGCTGACACAGATCAAACGCCAAGAGATGGATTCTCAGGTTAGGGTGCTAGAGCTAGAAAATGAATTG CAGAAGGAGCGTCAAAAACTGGGAGAGCTTCGGAAAAAGCACTACGAGCTTGCTGGTGTTGCTGAGGGC TGGGAAGAAGGAACAGAGGCATCTCCACCTACACTGCAAGAAGTGGTAACCGAAAAAGAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001243198
Insert Size	2961 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243198.2
RefSeq Size	7903 bp
RefSeq ORF	2961 bp
Locus ID	3092
UniProt ID	O00291
Protein Families	Druggable Genome
Protein Pathways	Huntington's disease
MW	110.7 kDa
Gene Summary	The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) lacks two alternate in-frame exons, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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