PDE4A (NM_001243121) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DPDE2; PDE4; PDE46
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331949 representing NM_001243121. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGAGGAGTCGCAGTGCCCTGTCCGTGGCAGGGACCGGGGACGAGAGGTCGAGGGAGACCCCCGAA TCCGACCGTGCCAACATGCTGGGGGCCGACCTGCGTCGCCCTCGCCGCCGCCTCTCGTCCGGTCCTGGC CTGGGCTGGGCCCAGCCTGAGCCCTCGGACCCTGGGGTCCCTCTGCCGCCACGGCCCACCACCCTGCCG CTGCTGATCCCACCGCGGATTTCCATCACCAGGGCCGAGAACGACAGCTTCGAGGCAGAGAATGGGCCG ACACCATCTCCTGGCCGCAGCCCCCTGGACTCGCAGGCGAGCCCAGGACTCGTGCTGCACGCCGGGGCG GCCACCAGCCAGCGCCGGGAGTCCTTCCTGTACCGCTCAGACAGCGACTATGACATGTCACCCAAGACC ATGTCCCGGAACTCATCGGTCACCAGCGAGGCGCACGCTGAAGACCTCATCGTAACACCATTTGCTCAG GTGCTGGCCAGCCTCCGGAGCGTCCGTAGCAACTTCTCACTCCTGACCAATGTGCCCGTTCCCAGTAAC AAGCGGTCCCCGCTGGGCGGCCCCACCCCTGTCTGCAAGGCCACGCTGTCAGAAGAAACGTGTCAGCAG TTGGCCCGGGAGACTCTGGAGGAGCTGGACTGGTGTCTGGAGCAGCTGGAGACCATGCAGACCTATCGC TCTGTCAGCGAGATGGCCTCGCACAAGTTCAAAAGGATGTTGAACCGTGAGCTCACACACCTGTCAGAA ATGAGCAGGTCCGGAAACCAGGTCTCAGAGTACATTTCCACAACATTCCTGGACAAACAGAATGAAGTG GAGATCCCATCACCCACGATGAAGGAACGAGAAAAACAGCAAGCGCCGCGACCAAGACCCTCCCAGCCG CCCCCGCCCCCTGTACCACACTTACAGCCCATGTCCCAAATCACAGGGTTGAAAAAGTTGATGCATAGT AACAGCCTGAACAACTCTAACATTCCCCGATTTGGGGTGAAGACCGATCAAGAAGAGCTCCTGGCCCAA GAACTGGAGAACCTGAACAAGTGGGGCCTGAACATCTTTTGCGTGTCGGATTACGCTGGAGGCCGCTCA CTCACCTGCATCATGTACATGATATTCCAGGAGCGGGACCTGCTGAAGAAATTCCGCATCCCTGTGGAC ACGATGGTGACATACATGCTGACGCTGGAGGATCACTACCACGCTGACGTGGCCTACCATAACAGCCTG CACGCAGCTGACGTGCTGCAGTCCACCCACGTACTGCTGGCCACGCCTGCACTAGATGCAGTGTTCACG GACCTGGAGATTCTCGCCGCCCTCTTCGCGGCTGCCATCCACGATGTGGATCACCCTGGGGTCTCCAAC CAGTTCCTCATCAACACCAATTCGGAGCTGGCGCTCATGTACAACGATGAGTCGGTGCTCGAGAATCAC CACCTGGCCGTGGGCTTCAAGCTGCTGCAGGAGGACAACTGCGACATCTTCCAGAACCTCAGCAAGCGC CAGCGGCAGAGCCTACGCAAGATGGTCATCGACATGGTGCTGGCCACGGACATGTCCAAGCACATGACC CTCCTGGCTGACCTGAAGACCATGGTGGAGACCAAGAAAGTGACCAGCTCAGGGGTCCTCCTGCTAGAT AACTACTCCGACCGCATCCAGGTCCTCCGGAACATGGTGCACTGTGCCGACCTCAGCAACCCCACCAAG CCGCTGGAGCTGTACCGCCAGTGGACAGACCGCATCATGGCCGAGTTCTTCCAGCAGGGTGACCGAGAG CGCGAGCGTGGCATGGAAATCAGCCCCATGTGTGACAAGCACACTGCCTCCGTGGAGAAGTCTCAGGTG GGTTTTATTGACTACATTGTGCACCCATTGTGGGAGACCTGGGCGGACCTTGTCCACCCAGATGCCCAG GAGATCTTGGACACTTTGGAGGACAACCGGGACTGGTACTACAGCGCCATCCGGCAGAGCCCATCTCCG CCACCCGAGGAGGAGTCAAGGGGGCCAGGCCACCCACCCCTGCCTGACAAGTTCCAGTTTGAGCTGACG CTGGAGGAGGAAGAGGAGGAAGAAATATCAATGGCCCAGATACCGTGCACAGCCCAAGAGGCATTGACT GCGCAGGGATTGTCAGGAGTCGAGGAAGCTCTGGATGCAACCATAGCCTGGGAGGCATCCCCGGCCCAG GAGTCGTTGGAAGTTATGGCACAGGAAGCATCCCTGGAGGCCGAGCTGGAGGCAGTGTATTTGACACAG CAGGCACAGTCCACAGGCAGTGCACCTGTGGCTCCGGATGAGTTCTCGTCCCGGGAGGAATTCGTGGTT GCTGTAAGCCACAGCAGCCCCTCTGCCCTGGCTCTTCAAAGCCCCCTTCTCCCTGCTTGGAGGACCCTG TCTGTTTCAGAGCATGCCCCGGGCCTCCCGGGCCTCCCCTCCACGGCGGCCGAGGTGGAGGCCCAACGA GAGCACCAGGCTGCCAAGAGGGCTTGCAGTGCCTGCGCAGGGACATTTGGGGAGGACACATCCGCACTC CCAGCTCCTGGTGGCGGGGGGTCAGGTGGAGACCCTACCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001243121
Insert Size	2595 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243121.1
RefSeq Size	4704 bp
RefSeq ORF	2595 bp
Locus ID	5141
UniProt ID	P27815
Protein Families	Druggable Genome
Protein Pathways	Progesterone-mediated oocyte maturation, Purine metabolism
MW	95.6 kDa
Gene Summary	The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (5) contains additional exons at the 5' end compared to variant 1. It encodes a shorter isoform (5, also known as PDE4A8) with a distinct N-terminus compared to isoform 1. This isoform is expressed predominantly in skeletal muscle and brain, a pattern that differs from that of other isoforms, and thus may have a specific function in regulating cAMP levels in human skeletal muscle and brain (PMID:18095939). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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