PARN (NM_001242992) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DAN; DKCB6; PFBMFT4
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331938 representing NM_001242992. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCCGAC TTCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGT TTTGACACTCCAGAAGAGAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTT CTAGCAAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAA GAAAGACAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTAT GTATCTCCTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTG GTAGAGAAAATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGG TTCCAAAGAAAACTAATTTATCAGACTTTGAGCTGGAAGTATCCGAAAGGCATTCATGTTGAGACTTTA GAAACTGAAAAGAAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAG CAGCAGAAACATGCCAAAGAACAGGAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCC ATTGCTAATTCGGGAAAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAG TTCTACTGCCCTCTGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGACTC TTGGATACTAAATTGATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACATCCCTTGCGGAA TTGGAAAAGCGGTTAAAAGAGACACCTTTCAACCCTCCTAAAGTTGAAAGTGCCGAAGGTTTTCCAAGT TATGACACAGCCTCTGAACAACTCCACGAGGCAGGCTACGATGCCTACATCACAGGGCTGTGCTTCATC TCCATGGCCAATTACCTAGGTTCTTTTCTCAGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTC ATTGAACCTTTTTTTAACAAGTTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGA CCAGACTTGCAGCCTAAACGTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGAC CTTTACCAGCTTTTCAGTGCCTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCATTTGTT TCCCTTAGCCAGCCCGAGCAAGTAAAGATTGCTGTCAATACCAGCAAATATGCAGAAAGCTATCGGATC CAAACCTATGCTGAATATATGGGGAGAAAACAGGAAGAGAAGCAGATCAAAAGAAAGTGGACTGAAGAT AGCTGGAAGGAGGCTGACAGCAAACGGTTAAACCCCCAGTGCATACCCTACACCCTGCAGAATCACTAT TACCGCAACAATAGTTTTACAGCTCCCAGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAA GCTGGCCTGGAGGACGGAGTGTCAGGGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCA GAGCCCCTCTCAGAGGGAAGGAAAAAGGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCA GGAAGCATCTCGAAGAACAGCCCTGCCACACTCTTTGAAGTTCCTGACACATGGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242992
Insert Size	1782 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242992.1
RefSeq Size	2945 bp
RefSeq ORF	1782 bp
Locus ID	5073
UniProt ID	O95453
Protein Families	Transcription Factors
Protein Pathways	RNA degradation
MW	67.7 kDa
Gene Summary	The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] Transcript Variant: This variant (3) uses two alternate in-frame splice sites and lacks an alternate exon in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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