SEPTIN7 (NM_001242956) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDC3; CDC10; NBLA02942; SEPT7; SEPT7A
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331936 representing NM_001242956. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCGGTCAGTGCGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCACCATGGGTGAATCT GGATTGGGAAAGTCGACATTAATCAACTCATTATTCCTCACAGATTTGTATTCTCCAGAGTATCCAGGT CCTTCTCATAGAATTAAAAAGACTGTACAGGTGGAACAATCCAAAGTTTTAATCAAAGAAGGTGGTGTT CAGTTGCTGCTCACAATAGTTGATACCCCAGGATTTGGAGATGCAGTGGATAATAGTAATTGCTGGCAG CCTGTTATCGACTACATTGATAGTAAATTTGAGGACTACCTAAATGCAGAATCACGAGTGAACAGACGT CAGATGCCTGATAACAGGGTGCAGTGTTGTTTATACTTCATTGCTCCTTCAGGACATGGACTTAAACCA TTGGATATTGAGTTTATGAAGCGTTTGCATGAAAAAGTGAATATCATCCCACTTATTGCCAAAGCAGAC ACACTCACACCAGAGGAATGCCAACAGTTTAAAAAACAGATAATGAAAGAAATCCAAGAACATAAAATT AAAATATACGAATTTCCAGAAACAGATGATGAAGAAGAAAATAAACTTGTTAAAAAGATAAAGGACCGT TTACCTCTTGCTGTGGTAGGTAGTAATACTATCATTGAAGTTAATGGCAAAAGGGTCAGAGGAAGGCAG TATCCTTGGGGTGTTGCTGAAGTTGAAAATGGTGAACATTGTGATTTTACAATCCTAAGAAATATGTTG ATAAGAACACACATGCAGGACTTGAAAGATGTTACTAATAATGTCCACTATGAGAACTACAGAAGCAGA AAACTTGCAGCTGTGACTTATAATGGAGTTGATAACAACAAGAATAAAGGGCAGCTGACTAAGAGCCCT CTGGCACAAATGGAAGAAGAAAGAAGGGAGCATGTAGCTAAAATGAAGAAGATGGAGATGGAGATGGAG CAGGTGTTTGAGATGAAGGTCAAAGAAAAAGTTCAAAAACTGAAGGACTCTGAAGCTGAGCTCCAGCGG CGCCATGAGCAAATGAAAAAGAATTTGGAAGCACAGCACAAAGAATTGGAGGAAAAACGTCGTCAGTTC GAGGATGAGAAAGCAAACTGGGAAGCTCAACAACGTATTTTAGAACAACAGAACTCTTCAAGAACCTTG GAAAAGAACAAGAAGAAAGGGAAGATCTTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242956
Insert Size	1206 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242956.1
RefSeq Size	4272 bp
RefSeq ORF	1206 bp
Locus ID	989
MW	46.6 kDa
Gene Summary	This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011] Transcript Variant: This variant (3) lacks an in-frame segment in the 5' coding region, compared to variant 1, resulting in an isoform (3) that is 36 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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