SAPS2 (PPP6R2) (NM_001242898) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA0685; PP6R2; SAP190; SAPS2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331919 representing NM_001242898. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTCTGGAAGTTTGACTTGAACACCACGTCCCATGTTGACAAGCTGCTGGACAAGGAGCATGTGACG CTGCAGGAGTTAATGGATGAAGATGACATCTTGCAGGAGTGTAAGGCTCAGAACCAGAAGCTGCTGGAC TTCCTGTGCAGGCAGCAGTGCATGGAGGAGCTGGTGAGCCTCATCACACAGGATCCGCCCCTGGACATG GAGGAGAAGGTCCGCTTCAAATATCCAAACACAGCCTGTGAGCTTCTGACTTGTGATGTGCCGCAGATC AGCGACCGCCTCGGTGGGGACGAGAGCCTGCTGAGCCTCCTGTACGACTTCTTGGACCATGAGCCGCCT CTCAATCCTCTGCTCGCCAGTTTTTTCAGCAAGACCATTGGCAATCTCATTGCAAGAAAAACCGAACAG GTGATTACGTTTTTGAAGAAGAAGGACAAGTTCATCAGCCTGGTGTTGAAGCACATCGGCACCTCAGCG CTTATGGACCTGCTGCTGCGCCTGGTCAGCTGTGTGGAGCCAGCCGGGCTCCGGCAGGACGTCCTGCAC TGGCTGAATGAAGAGAAGGTCATCCAGAGGCTTGTGGAGTTGATCCACCCGAGCCAGGATGAAGATAGG CAGTCAAATGCTTCTCAGACTCTCTGTGACATAGTTAGGCTGGGCAGAGACCAGGGCAGTCAGCTGCAA GAGGCTCTGGAGCCAGACCCGCTCCTCACAGCGCTGGAGTCGCAGGACTGTGTGGAGCAGCTTCTGAAG AACATGTTTGATGGAGACCGGACGGAGAGCTGCCTCGTCAGTGGGACTCAGGTGTTACTCACCTTGCTG GAAACCAGGCGGGTTGGGACAGAGGGCTTGGTGGACTCCTTTTCTCAGGGACTGGAAAGGTCATACGCT GTCAGCAGCAGCGTACTACACGGCATCGAGCCTCGGCTGAAGGACTTCCACCAGCTCCTGCTCAACCCG CCCAAGAAGAAAGCGATCCTGACCACCATTGGTGTGCTGGAGGAGCCCCTGGGGAATGCCCGTCTGCAT GGCGCCCGCCTCATGGCAGCACTGCTGCACACAAACACACCCAGCATCAACCAGGAGCTCTGCCGGCTC AACACGATGGACTTACTGCTGGACTTGTTCTTTAAGTACACCTGGAATAACTTTTTGCACTTCCAAGTG GAACTATGCATAGCCGCTATTCTCTCCCACGCTGCCCGTGAGGAGAGGACAGAAGCCAGCGGATCCGAG AGCAGGGTGGAGCCTCCGCATGAGAACGGGAACCGGAGCCTGGAGACTCCCCAGCCGGCCGCCAGCCTC CCTGACAACACAATGGTGACCCACCTGTTCCAGAAGTGCTGCCTGGTGCAGAGGATCCTGGAGGCCTGG GAAGCCAACGACCACACGCAGGCAGCGGGTGGCATGAGACGTGGGAACATGGGCCACCTCACACGGATC GCCAACGCGGTGGTGCAGAACCTGGAGCGGGGCCCTGTGCAGACGCACATCAGCGAGGTCATCCGAGGG CTCCCTGCGGACTGCCGTGGCCGCTGGGAGAGCTTCGTGGAGGAGACGCTGACGGAGACGAACCGCAGG AACACTGTGGACCTGGTGAGCACTCACCACCTTCACTCCTCAAGTGAGGACGAGGACATTGAGGGTGCT TTCCCTAACGAGCTGTCCCTTCAGCAGGCCTTCTCTGACTACCAGATCCAGCAGATGACAGCCAACTTC GTGGATCAGTTTGGCTTCAATGATGAGGAGTTTGCCGACCAGGACGACAACATCAATGCCCCGTTTGAC AGGATCGCAGAGATCAACTTCAACATCGACGCTGACGAGGACAGTCCCAGCGCAGCTCTGTTTGAGGCC TGCTGCAGTGACCGCATCCAGCCCTTTGATGATGATGAGGACGAGGACATCTGGGAGGACAGTGACACT CGCTGTGCTGCCCGGGTGATGGCCAGACCCAGGTTTGGAGCCCCCCATGCTTCAGAGAGTTGCTCAAAG AATGGCCCAGAGCGTGGAGGCCAGGATGGGAAGGCGAGCTTGGAAGCACACAGAGATGCACCTGGGGCA GGTGCCCCACCGGCCCCCGGGAAGAAGGAAGCGCCCCCTGTGGAGGGTGACTCAGAAGGCGCCATGTGG ACGGCAGTGTTTGATGAGCCAGCGAACTCAACGCCCACAGCCCCAGGAGTGGTGAGGGACGTGGGTTCC AGTGTGTGGGCAGCTGGCACCTCAGCTCCAGAGGAGAAAGGCTGGGCCAAGTTCACTGACTTCCAACCT TTCTGCTGCTCCGAGTCAGGGCCCAGGTGCAGCTCTCCGGTGGACACAGAATGCAGCCATGCTGAGGGC AGCCGGAGCCAAGGCCCTGAGAAAGCCTTCAGCCCGGCTTCTCCATGTGCCTGGAACGTGTGTGTCACC AGGAAGGCCCCCCTGCTGGCCTCTGACAGTAGCTCCTCTGGGGGCTCCCACAGCGAGGATGGCGACCAG AAGGCAGCGAGTGCCATGGATGCGGTGAGCAGGGGTCCCGGCCGGGAGGCCCCCCCGCTGCCCACAGTG GCCAGGACAGAGGAGGCTGTCGGCAGGGTCGGGTGTGCTGACAGCCGGCTGTTAAGCCCTGCCTGCCCC GCGCCAAAGGAAGTGACTGCTGCCCCAGCCGTGGCTGTGCCCCCCGAGGCTACTGTGGCCATCACCACA GCACTGAGCAAGGCTGGCCCCGCCATACCCACCCCAGCAGTCTCTTCTGCACTGGCCGTGGCGGTCCCC CTAGGGCCCATCATGGCAGTCACAGCAGCCCCAGCCATGGTGGCCACCCTGGGGACAGTGACAAAGGAC GGGAAGACAGATGCCCCGCCAGAAGGAGCTGCCTTAAATGGCCCAGTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001242898
Insert Size	2880 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242898.1
RefSeq Size	4104 bp
RefSeq ORF	2880 bp
Locus ID	9701
UniProt ID	O75170
MW	104.2 kDa
Gene Summary	The protein encoded by this gene is a regulatory protein for the protein phosphatase-6 catalytic subunit. Together, these proteins act as a significant T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of function of either the regulatory or catalytic subunit of protein phosphatase-6 interferes with spindle formation and chromosome alignment. [provided by RefSeq, May 2017] Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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