RNF146 (NM_001242851) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331904 representing NM_001242851. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATGGCTGGCTGTGGTGAAATTGATCATTCAATAAACATGCTTCCTACAAACAGGAAAGCGAACGAG TCCTGTTCTAATACTGCACCTTCTTTAACCGTCCCTGAATGTGCCATTTGTCTGCAAACATGTGTTCAT CCAGTCAGTCTGCCCTGTAAGCACGTTTTCTGCTATCTATGTGTAAAAGGAGCTTCATGGCTTGGAAAG CGGTGTGCTCTTTGTCGACAAGAAATTCCCGAGGATTTCCTTGACAAGCCAACCTTGTTGTCACCAGAA GAACTCAAGGCAGCAAGTAGAGGAAATGGTGAATATGCATGGTATTATGAAGGAAGAAATGGGTGGTGG CAGTACGATGAGCGCACTAGTAGAGAGCTGGAAGATGCTTTTTCCAAAGGTAAAAAGAACACTGAAATG TTAATTGCTGGCTTTCTGTATGTCGCTGATCTTGAAAACATGGTTCAATATAGGAGAAATGAACATGGA CGTCGCAGGAAGATTAAGCGAGATATAATAGATATACCAAAGAAGGGAGTAGCTGGACTTAGGCTAGAC TGTGATGCTAATACCGTAAACCTAGCAAGAGAGAGCTCTGCTGACGGAGCGGACAGTGTATCAGCACAG AGTGGAGCTTCTGTTCAGCCCCTAGTGTCTTCTGTAAGGCCCCTAACATCAGTAGATGGTCAGTTAACA AGCCCTGCAACACCATCCCCTGATGCAAGCACTTCTCTGGAAGACTCTTTTGCTCATTTACAACTCAGT GGAGACAACACAGCTGAAAGGAGTCATAGGGGAGAAGGAGAAGAAGATCATGAATCACCATCTTCAGGC AGGGTACCAGCACCAGACACCTCCATTGAAGAAACTGAATCAGATGCCAGTAGTGATAGTGAGGATGTA TCTGCAGTTGTTGCACAGCACTCCTTGACCCAACAGAGACTTTTGGTTTCTAATGCAAACCAGACAGTA CCCGATCGATCAGATCGATCGGGAACTGATCGATCAGTAGCAGGGGGTGGAACAGTGAGTGTCAGTGTC AGATCTAGAAGGCCTGATGGACAGTGCACAGTAACTGAAGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242851
Insert Size	1080 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242851.1
RefSeq Size	2103 bp
RefSeq ORF	1080 bp
Locus ID	81847
UniProt ID	Q9NTX7
Protein Families	Druggable Genome
MW	39 kDa
Gene Summary	E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (10) contains an alternate 5' terminal exon and lacks two alternate internal exons, which results in the use of an upstream start codon, compared to variant 1. The encoded isoform (b) has a longer N-terminus than isoform a. Variants 8-10 encode the same isoform (b).

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