l Afadin (AFDN) (NM_001207008) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AF6; l-afadin; MLL-AF6; MLLT4
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331731 representing NM_001207008. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCGGCGGGCGGCCGTGACGAGGAGCGGCGGAAGCTGGCCGACATCATCCACCACTGGAACGCCAAC CGGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTCCATGGAGTGATGAGATTTTAT TTTCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGTACTGCCACCACTCAA GATGTAATCGAAACGCTCGCGGAGAAATTTCGACCTGATATGCGAATGCTGTCCTCTCCCAAGTATTCA CTCTATGAAGTGCATGTCAGCGGAGAAAGAAGATTGGATATAGATGAGAAACCTCTAGTTGTACAACTG AATTGGAACAAAGATGATCGGGAAGGCAGATTTGTTCTTAAGAATGAGAATGACGCCATTCCTCCTAAG GCTCAAAGTAATGGACCTGAAAAGCAGGAAAAAGAAGGGGTTATCCAGAACTTCAAGAGAACTCTCTCA AAGAAAGAAAAGAAGGAAAAAAAGAAGAGAGAAAAAGAGGCATTGCGACAGGCATCTGATAAAGATGAT AGACCTTTCCAAGGGGAGGATGTTGAAAATTCTCGACTGGCTGCTGAGGTTTACAAAGACATGCCGGAA ACCAGCTTTACTCGAACCATTTCTAATCCTGAGGTGGTTATGAAACGACGGAGGCAGCAAAAATTGGAA AAGAGAATGCAGGAATTTCGGAGCTCAGATGGGCGGCCTGATTCAGGTGGAACATTGAGAATTTATGCA GATAGTTTAAAACCAAATATTCCCTACAAGACAATCCTGCTGTCTACTACAGATCCTGCAGACTTTGCT GTGGCTGAAGCTTTAGAGAAGTATGGTCTGGAAAAAGAAAACCCTAAGGATTACTGCATCGCCCGGGTT ATGCTTCCTCCTGGAGCCCAGCATTCTGATGAAAAGGGTGCTAAAGAAATTATTCTTGATGATGATGAG TGTCCTTTACAAATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGG CCACCAGACCACATCCCAAAGAAAACCAAGAAACACTTGGAAGGCAAGACACCCAAGGGAAAGGAGAGA GCTGACGGGTCTGGCTATGGCTCCACCCTTCCTCCGGAGAAGCTGCCCTATTTAGTAGAGTTAAGCCCA GATGGTTCTGACTCTAGAGATAAGCCAAAGCTTTACCGCCTTCAGTTAAGTGTTACTGAAGTTGGGACA GAAAAGTTGGATGACAACTCTATCCAGTTGTTTGGCCCAGGAATTCAGCCCCATCACTGTGACCTTACC AACATGGATGGAGTGGTCACTGTGACGCCCAGAAGTATGGACGCAGAAACCTACGTGGAAGGCCAGCGC ATCTCAGAAACCACCATGCTGCAGAGTGGCATGAAAGTGCAGTTTGGGGCGTCCCATGTATTTAAGTTT GTGGACCCCAGTCAGGATCATGCTCTTGCAAAAAGATCTGTGGATGGAGGCCTGATGGTTAAGGGCCCA AGACATAAACCTGGAATTGTTCAGGAGACAACTTTTGATTTGGGAGGAGATATTCATAGTGGGACAGCA TTACCGACAAGCAAGAGCACCACTAGGCTGGACAGCGACAGAGTGTCGTCTGCCTCTAGCACAGCCGAG CGGGGAATGGTGAAGCCGATGATCAGAGTAGAACAGCAGCCAGATTATCGCAGGCAAGAAAGCAGAACA CAGGATGCTTCTGGGCCTGAGCTGATACTACCTGCAAGCATTGAATTCAGGGAAAGTTCTGAAGATTCA TTTTTGTCTGCCATTATAAATTATACTAATAGCTCTACAGTCCACTTTAAGTTGTCCCCTACATATGTA TTATATATGGCATGCCGGTATGTATTGTCCAACCAGTACAGACCTGACATCAGCCCTACAGAGCGCACA CATAAAGTCATTGCAGTCGTCAACAAGATGGTGAGCATGATGGAGGGTGTCATCCAGAAACAGAAGAAT ATTGCAGGGGCACTTGCCTTCTGGATGGCAAATGCATCTGAACTTCTCAACTTCATTAAGCAAGACCGA GACCTTAGTCGGATCACACTGGATGCTCAAGATGTTTTAGCACATTTGGTTCAAATGGCATTTAAATAC TTGGTTCACTGTCTTCAATCAGAACTTAATAATTACATGCCAGCCTTTCTAGATGACCCTGAAGAGAAC AGTCTGCAACGACCAAAAATAGATGATGTGCTGCACACGCTCACAGGAGCCATGTCCTTGCTACGACGC TGCAGAGTCAATGCCGCCCTGACCATCCAGCTCTTCTCTCAGCTCTTCCACTTCATCAATATGTGGCTG TTCAATAGATTGGTGACCGACCCAGATTCGGGGCTGTGCTCCCATTACTGGGGTGCGATTATCCGTCAG CAGTTGGGCCATATTGAAGCCTGGGCTGAGAAGCAGGGGCTGGAACTGGCTGCGGACTGTCATCTGAGC AGGATCGTGCAGGCAACGACTTTGCTTACCATGGATAAGTATGCACCTGATGACATTCCAAATATAAAC AGCACCTGCTTTAAGTTAAATTCATTACAACTTCAAGCCTTATTACAGAACTATCACTGTGCACCTGAT GAGCCTTTTATCCCAACGGATCTTATAGAAAATGTAGTGACTGTGGCTGAAAACACTGCCGATGAGCTG GCCCGCAGTGATGGAAGGGAAGTGCAGTTGGAGGAGGATCCTGATCTGCAGCTGCCGTTTCTTTTGCCA GAAGATGGTTATTCTTGTGATGTTGTCAGAAACATTCCAAATGGTTTACAAGAATTTTTAGACCCTCTG TGCCAGAGAGGATTTTGCAGGTTAATTCCTCACACACGTTCACCAGGTACTTGGACAATATATTTTGAA GGTGCAGATTATGAAAGTCACCTTCTGCGTGAGAACACAGAGCTGGCTCAGCCTCTGAGGAAAGAACCT GAAATAATCACTGTGACCCTAAAAAAGCAGAATGGAATGGGCCTTAGCATTGTTGCAGCAAAGGGTGCT GGTCAAGATAAACTAGGAATCTATGTGAAGTCGGTTGTGAAAGGAGGTGCTGCAGATGATGGACGTCTA GCTGCAGGTGATCAGCTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACTCTCTCAGGAAAGGGCGGCA GAACTCATGACAAGAACAAGCTCTGTGGTGACACTGGAAGTAGCAAAGCAGGGTGCCATCTACCACGGT CTGGCCACCCTTCTCAATCAGCCATCCCCCATGATGCAGAGAATTTCAGATCGTCGTGGCTCAGGTAAA CCCCGACCAAAGAGTGAAGGCTTTGAGCTCTATAATAATTCAACTCAAAATGGGTCTCCTGAGAGTCCT CAGCTGCCTTGGGCAGAATATAGTGAACCAAAGAAATTGCCTGGTGATGACAGACTGATGAAAAATAGA GCTGATCACCGTTCCAGCCCCAACGTAGCAAATCAGCCTCCTAGTCCTGGAGGGAAAAGTGCATATGCC TCTGGAACAACAGCGAAGATAACATCTGTCTCTACTGGAAACCTCTGCACTGAGGAGCAGACGCCTCCG CCTAGACCTGAAGCCTACCCCATCCCCACTCAGACGTACACCAGAGAGTATTTTACCTTCCCAGCTTCC AAATCCCAGGATCGGATGGCTCCTCCTCAGAACCAGTGGCCAAATTATGAGGAAAAGCCACATATGCAC ACAGATAGTAATCATTCCAGTATTGCAATTCAGCGTGTTACACGTTCCCAAGAAGAACTTCGAGAAGAT AAAGCTTACCAACTTGAGCGGCATCGAATAGAGGCAGCTATGGACCGAAAGTCTGATAGTGATATGTGG ATAAATCAGAGCTCCTCACTGGACTCCAGTACCTCTAGCCAGGAGCATCTGAACCATTCCTCTAAGTCG GTCACCCCTGCTTCCACACTGACCAAAAGTGGCCCTGGCCGTTGGAAAACACCAGCAGCCATACCGGCC ACCCCTGTGGCCGTCTCCCAGCCAATCCGAACAGACCTGCCTCCGCCACCCCCGCCACCTCCAGTCCAC TATGCCGGTGATTTCGATGGAATGTCCATGGATTTGCCTCTCCCACCACCCCCTTCCGCCAACCAGATA GGGCTGCCGTCTGCGCAGGTGGCTGCTGCTGAACGGAGAAAGAGAGAAGAACATCAGCGTTGGTATGAG AAGGAGAAGGCCCGCCTGGAGGAGGAGCGGGAGAGGAAGCGGAGAGAGCAGGAGAGGAAGTTGGGCCAG ATGCGCACTCAGTCCTTAAACCCTGCTCCGTTTTCTCCCCTGACTGCACAGCAGATGAAGCCCGAAAAG CCTTCCACACTCCAGCGGCCACAGGAAACAGTCATTCGGGAGCTGCAGCCTCAGCAGCAGCCCCGCACG ATCGAGCGCAGAGACTTGCAGTACATTACAGTCAGCAAAGAGGAGCTTTCCTCGGGGGACAGTCTGTCC CCCGACCCGTGGAAGCGGGACGCCAAGGAGAAGCTGGAGAAGCAGCAGCAGATGCACATCGTGGACATG CTGAGCAAGGAGATCCAGGAGCTCCAGAGCAAACCGGACCGCAGCGCCGAGGAGAGCGACCGGCTGCGC AAGCTCATGCTGGAGTGGCAGTTCCAGAAGAGACTCCAGGAGTCGAAGCAGAAGGACGAAGATGACGAG GAGGAGGAGGACGATGATGTGGACACCATGCTGATCATGCAGCGCCTGGAGGCTGAACGAAGAGCGAGG TTGCAGGACGAGGAGCGGAGGCGGCAGCAGCAGTTAGAAGAGATGCGCAAGCGGGAAGCGGAAGACCGA GCGAGGCAAGAGGAAGAGCGCCGGCGGCAGGAGGAGGAGCGAACAAAACGAGACGCTGAAGAAAAGAGG CGACAGGAAGAAGGGTATTACAGCCGCCTGGAAGCCGAGAGGCGCAGACAGCACGACGAGGCGGCGCGC AGGTTGCTGGAGCCCGAGGCGCCCGGACCAAACTCTTACCCAGGATCTACTGGAGCAGCTGTTGGAGCC CATGACGCCTGTCGGGATGCAAAAGAGAAGCGCTCTAAAAGCCAAGATGCAGATTCACCTGGAAGTTCT GGGGCCCCTGAAAACTTGACATTCAAGGAACGCCAGCGTCTTTTTTCACAAGGTCAAGATGTATCCAAT AAAGTGAAAGCTTCTCGTAAATTAACAGAACTGGAGAATGAACTGAACACAAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001207008
Insert Size	5232 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001207008.1
RefSeq Size	7459 bp
RefSeq ORF	5232 bp
Locus ID	4301
UniProt ID	P55196
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Leukocyte transendothelial migration, Tight junction
MW	197.7 kDa
Gene Summary	This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011] Transcript Variant: This variant (1) has multiple differences compared to variant 4. The resulting isoform (1, also known as AF6i3) contains a C-terminal F-actin-binding site and has been shown to be involved in the regulation of epithelial wound closure and intercellular adhesion (PMID:16882694).

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