Thyroid Peroxidase (TPO) (NM_001206745) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MSA; TDH2A; TPX
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331678 representing NM_001206745. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGAGCGCTCGCTGTGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATC TCGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCTCGTGTCTCTAGCGTCTTGGAGGAAAGC AAGCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCT CCAGCTCAGCTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAG ATAATGGAAACATCAATACAAGCGATGAAAAGAAAAGTCAACCTGAAAACTCAACAATCACAGCATCCA ACGGATGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATGCTG CCCCCAAAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGA GACCACCCCAGATGGGGCGCCTCCAACACGGCCCTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGC TTCAGTCAGCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCCGGGAG GTGACAAGACATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTG ATGGCATGGGGACAATACATCGACCACGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTC GGGGGAGGGGCTGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATACAACTCCCGGAG GAGGCCCGGCCGGCCGCGGGCACCGCCTGTCTGCCCTTCTACCGCTCTTCGGCCGCCTGCGGCACCGGG GACCAAGGCGCGCTCTTTGGGAACCTGTCCACGGCCAACCCGCGGCAGCAGATGAACGGGTTGACCTCG TTCCTGGACGCGTCCACCGTGTATGGCAGCTCCCCGGCCCTAGAGAGGCAGCTGCGGAACTGGACCAGT GCCGAAGGGCTGCTCCGCGTCCACGCGCGCCTCCGGGACTCCGGCCGCGCCTACCTGCCCTTCGTGCCG CCACGCGCGCCTGCGGCCTGTGCGCCCGAGCCCGGCATCCCCGGAGAGACCCGCGGGCCCTGCTTCCTG GCCGGAGACGGCCGCGCCAGCGAGGTCCCCTCCCTGACGGCACTGCACACGCTGTGGCTGCGCGAGCAC AACCGCCTGGCCGCGGCGCTCAAGGCCCTCAATGCGCACTGGAGCGCGGACGCCGTGTACCAGGAGGCG CGCAAGGTCGTGGGCGCTCTGCACCAGATCATCACCCTGAGGGATTACATCCCCAGGATCCTGGGACCC GAGGCCTTCCAGCAGTACGTGGGTCCCTATGAAGGCTATGACTCCACCGCCAACCCCACTGTGTCCAAC GTGTTCTCCACAGCCGCCTTCCGCTTCGGCCATGCCACGATCCACCCGCTGGTGAGGAGGCTGGACGCC AGCTTCCAGGAGCACCCCGACCTGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTA CTCCGTGGAGGTTACAATGAGTGGAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCCCCGCTGACCTG AGCACAGCCATCGCCAGCAGGAGCGTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATC GATGTCTGGCTGGGAGGCTTAGCTGAAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTGTTTGCCTGT CTCATTGGGAAGCAGATGAAGGCTCTGCGGGACGGTGACTGGTTTTGGTGGGAGAACAGCCACGTCTTC ACGGATGCACAGAGGCGTGAGCTGGAGAAGCACTCCCTGTCTCGGGTCATCTGTGACAACACTGGCCTC ACCAGGGTGCCCATGGATGCCTTCCAAGTCGGCAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATC ACTGGCATGAACCTGGAGGCCTGGAGGGAAACCTTTCCTCAAGACGACAAGTGTGGCTTCCCAGAGAGC GTGGAGAATGGGGACTTTGTGCACTGTGAGGAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCAC GGGTATGAGCTCCAAGGCCGGGAGCAGCTCACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTC TGCAAAGATGTGAACGAGTGTGCAGACGGTGCCCACCCCCCCTGCCACGCCTCTGCGAGGTGCAGAAAC ACCAAAGGCGGCTTCCAGTGTCTCTGCGCGGACCCCTACGAGTTAGGAGACGATGGGAGAACCTGCGTA GACTCCGGGAGGCTCCCTCGGGTGACTTGGATCTCCATGTCGCTGGCTGCTCTGCTGATCGGAGGCTTC GCAGGTCTCACCTCGACGGTGATTTGCAGGTGGACACGCACTGGCACTAAATCCACACTGCCCATCTCG GAGACAGGCGGAGGAACTCCCGAGCTGAGATGCGGAAAGCACCAGGCCGTAGGGACCTCACCGCAGCGG GCCGCAGCTCAGGACTCGGAGCAGGAGAGTGCTGGGATGGAAGGCCGGGATACTCACAGGCTGCCGAGA GCCCTCTGA
Restriction Sites	SgfI-NotI
ACCN	NM_001206745
Insert Size	2631 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206745.1
RefSeq Size	2980 bp
RefSeq ORF	2631 bp
Locus ID	7173
UniProt ID	P07202
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Metabolic pathways, Tyrosine metabolism
MW	96.7 kDa
Gene Summary	This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011] Transcript Variant: This variant (7) lacks a segment in the 5' UTR and an in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b, also known as TPO2) that is shorter than isoform a.

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