ANO6 (NM_001204803) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BDPLT7; SCTS; TMEM16F
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331608 representing NM_001204803. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAAAAGATGAGCAGGAATGTTTTGCTACAAATGGAGGAGGAGGAGGACGACGACGATGGGGATATC GGTGATGTTCCTGCCTCCAGGAGGCCTTTTCTGACTCCTCATACTCACCTCCCATCCAGCCTGGTGTTG GAAAACCTTGGACAGACAATTGTCCCCGATTTGGGATCACTGGAAAGTCAGCATGATTTTCGAACCCCG GAGTTTGAAGAATTTAATGGAAAACCTGACTCCCTCTTTTTTAATGATGGCCAGCGAAGAATTGACTTT GTTCTAGTATATGAGGATGAAAGCAGAAAAGAGACCAATAAAAAGGGTACAAATGAAAAACAAAGGAGG AAAAGACAAGCATACGAATCTAACCTTATCTGTCATGGCCTGCAGTTAGAAGCAACAAGATCAGTATTG GATGACAAGCTTGTATTTGTAAAAGTACACGCACCATGGGAGGTGTTATGTACGTATGCTGAGATAATG CACATCAAATTGCCTCTGAAACCCAATGATCTGAAAAACCGGTCCTCAGCCTTTGGTACACTCAACTGG TTTACCAAAGTCCTCAGTGTAGACGAAAGCATCATCAAGCCAGAGCAAGAGTTTTTCACTGCCCCATTT GAGAAGAACCGGATGAATGATTTTTACATAGTTGATAGAGATGCTTTCTTCAATCCAGCCACCAGAAGC CGCATTGTTTACTTCATCCTCTCTCGGGTCAAGTATCAAGTGATAAACAATGTTAGCAAGTTTGGGATC AACAGACTTGTAAACTCTGGGATCTACAAGGCAGCTTTCCCACTCCATGATTGCAAATTCCGCCGTCAG TCAGAGGATCCCAGCTGCCCTAATGAACGGTACCTTCTGTACAGAGAATGGGCTCATCCTCGAAGCATA TACAAAAAGCAGCCCTTGGATCTTATCAGGAAATACTATGGAGAGAAGATTGGAATCTACTTTGCTTGG CTGGGCTATTACACTCAGATGCTTCTCCTGGCCGCAGTTGTAGGAGTGGCTTGCTTTCTCTATGGATAT CTTAATCAAGATAACTGTACATGGAGCAAAGAAGTTTGTCATCCTGATATTGGTGGCAAGATCATAATG TGTCCTCAGTGTGATAGGCTTTGTCCATTCTGGAAACTCAATATTACTTGCGAGTCCTCAAAGAAATTG TGCATCTTCGACAGTTTTGGAACCCTGGTCTTTGCAGTATTTATGGGAGTATGGGTTACCTTGTTTTTG GAGTTTTGGAAGCGACGCCAGGCAGAACTTGAGTATGAATGGGATACTGTTGAGTTACAGCAGGAAGAA CAAGCCCGACCAGAATACGAAGCACGATGTACTCACGTAGTGATAAATGAGATTACTCAGGAAGAAGAA CGCATTCCCTTTACTGCCTGGGGAAAATGTATACGGATAACCCTCTGTGCCAGTGCTGTCTTTTTCTGG ATCCTATTGATCATCGCTTCAGTTATTGGGATCATTGTCTATAGGCTCTCGGTGTTCATTGTATTTTCT GCAAAACTTCCCAAGAACATTAATGGAACAGACCCAATCCAGAAATACCTGACTCCACAGACAGCCACG TCCATCACGGCCTCCATCATCAGCTTTATAATTATCATGATTCTGAACACCATATATGAAAAAGTGGCA ATTATGATTACTAACTTCGAACTCCCAAGGACCCAGACTGATTATGAGAACAGCCTCACCATGAAGATG TTCTTATTCCAGTTTGTCAACTACTACTCTTCATGCTTCTACATAGCATTCTTTAAGGGCAAATTTGTA GGCTATCCAGGAGACCCAGTTTATTGGTTGGGAAAATACAGAAATGAAGAGTGTGACCCAGGTGGCTGT CTTCTTGAACTGACAACTCAGCTGACAATAATCATGGGAGGAAAAGCAATCTGGAATAACATACAAGAA GTATTATTGCCCTGGATCATGAATCTAATTGGGCGATTTCACAGAGTTTCTGGATCAGAAAAGATAACC CCACGATGGGAACAGGACTACCATCTGCAGCCTATGGGCAAACTGGGATTATTTTATGAATATCTTGAA ATGATTATTCAGTTTGGGTTCGTCACCTTATTTGTGGCCTCTTTTCCACTGGCCCCTCTGTTGGCTCTC GTGAACAATATATTGGAAATAAGAGTGGACGCATGGAAACTGACCACCCAGTTTAGACGCCTGGTACCA GAGAAAGCCCAAGACATTGGAGCATGGCAGCCCATCATGCAAGGAATAGCAATTCTGGCTGTGGTGACC AATGCCATGATCATAGCTTTCACGTCGGACATGATCCCCCGCCTAGTGTACTACTGGTCCTTCTCCGTC CCTCCCTACGGGGACCACACTTCCTACACCATGGAAGGGTACATCAACAACACTCTCTCCATCTTCAAA GTCGCAGACTTCAAAAACAAAAGCAAGGGAAACCCGTACTCTGACCTGGGTAACCATACCACATGCAGG TATCGTGATTTCCGATACCCACCTGGACACCCCCAGGAGTATAAACACAACATCTACTATTGGCATGTG ATTGCAGCCAAGCTGGCTTTTATCATTGTCATGGAGCACGTCATCTACTCTGTGAAATTTTTCATTTCA TATGCAATTCCCGATGTATCAAAACGCACAAAGAGCAAGATCCAGAGAGAAAAATACCTAACCCAAAAG CTTCTTCATGAGAATCACCTCAAAGATATGACGAAAAATATGGGGGTGATAGCTGAGCGGATGATAGAA GCAGTAGATAACAATTTACGGCCAAAATCAGAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204803
Insert Size	2796 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204803.1
RefSeq Size	6196 bp
RefSeq ORF	2796 bp
Locus ID	196527
UniProt ID	Q4KMQ2
Protein Families	Transmembrane
MW	108.4 kDa
Gene Summary	This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] Transcript Variant: This variant (5) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (d) with an internal protein segment not found in isoform a.

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