RFC1 (NM_001204747) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331606 representing NM_001204747. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACATTCGGAAATTCTTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAG AATGAGAAAACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAAT AGCTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATCTATGATTCA GATTCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCAGAAAAACTGCCAGTATCT TCTAAACCTGGTAAAATTTCACGGCAGGATCCTGTTACATACATTTCAGAAACAGATGAAGAAGATGAC TTTATGTGTAAGAAGGCGGCCTCTAAATCAAAAGAGAATGGAAGATCTACAAATAGTCATCTTGGAACA TCAAACATGAAAAAGAATGAAGAAAACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACA CCCACATCAGTACTTGATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGC AAAAGAAAAGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAATTA CAGCTTGATGAAGATGCGGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAGTTTGCCAGAACATTAGCC ATGTTGGATGAAGAACCCAAGACCAAAAAGGCTCGAAAGGACACAGAAGCGGGAGAAACGTTTTCATCT GTCCAAGCCAATTTAAGTAAAGCAGAAAAACATAAATATCCTCATAAAGTAAAAACAGCACAAGTTTCA GATGAAAGAAAGAGCTACAGTCCTAGGAAGCAAAGTAAATATGAAAGTTCAAAAGAATCTCAGCAACAT TCCAAGTCATCAGCTGACAAAATAGGAGAAGTCTCTTCTCCCAAGGCCAGTTCTAAGCTGGCAATTATG AAAAGAAAAGAAGAGAGCTCTTATAAAGAAATAGAGCCTGTGGCCTCAAAAAGAAAAGAAAATGCCATT AAATTGAAAGGAGAGACAAAAACTCCTAAGAAAACCAAAAGTTCTCCAGCTAAAAAAGAGTCTGTAAGT CCTGAAGATTCTGAAAAGAAACGCACTAATTATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCC AAGGCTCTGGGCTCCAAAGAAATACCAAAGGGAGCTGAAAATTGCTTGGAAGGCCTTATATTTGTAATC ACAGGCGTGCTGGAGTCTATTGAACGAGATGAGGCCAAGTCTCTAATTGAACGTTATGGGGGAAAAGTA ACAGGAAATGTCAGCAAGAAAACAAATTATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGAT AAGGCCGCAGCCTTGGGGACAAAAATTATTGATGAAGATGGCCTGTTGAATCTGATTCGGACTATGCCA GGCAAGAAATCCAAGTATGAAATAGCAGTTGAAACTGAGATGAAGAAAGAGTCCAAACTGGAGAGAACA CCCCAAAAAAATGTCCAAGGAAAAAGAAAAATTAGTCCATCTAAAAAGGAATCAGAATCTAAAAAGAGC AGGCCGACTTCCAAAAGGGACAGTTTGGCAAAGACAATAAAAAAGGAAACAGATGTGTTTTGGAAAAGC CTGGATTTCAAGGAGCAGGTGGCTGAGGAGACAAGTGGTGACAGCAAGGCTAGGAATTTGGCTGATGAC AGCAGTGAAAACAAAGTGGAAAATTTGCTCTGGGTGGATAAATATAAGCCAACCTCGCTCAAGACCATA ATTGGACAGCAAGGTGACCAGAGCTGTGCCAACAAACTCCTACGCTGGCTCCGAAACTGGCAAAAGAGT TCTTCCGAAGATAAAAAACACGCAGCAAAGTTTGGTAAATTTTCCGGCAAAGATGATGGCTCTAGTTTT AAAGCAGCGTTGCTGTCAGGCCCTCCTGGTGTTGGCAAAACCACCACAGCTTCCCTGGTGTGTCAGGAG TTGGGATACAGCTACGTGGAACTGAATGCAAGTGACACCCGGAGTAAGAGCAGTTTGAAGGCGATTGTT GCTGAGTCACTGAACAATACCAGCATCAAAGGCTTTTATTCAAATGGAGCAGCCTCTTCAGTAAGCACG AAACATGCTCTCATCATGGATGAAGTAGATGGCATGGCAGGCAATGAGGATAGGGGAGGAATTCAGGAA TTAATTGGCCTGATAAAACATACTAAAATTCCCATTATTTGTATGTGCAATGATAGAAATCATCCCAAG ATTCGCTCTCTGGTTCATTATTGTTTTGATCTTCGTTTTCAAAGACCTCGGGTTGAACAGATTAAGGGT GCTATGATGTCTATTGCATTTAAAGAAGGTTTAAAGATTCCCCCTCCAGCTATGAATGAAATAATTTTG GGAGCCAATCAAGATATCAGACAGGTTTTACATAATCTGAGTATGTGGTGTGCACGAAGTAAAGCATTA ACCTATGACCAGGCCAAAGCTGATTCTCACAGAGCCAAAAAGGATATCAAAATGGGCCCATTTGATGTT GCCCGGAAAGTGTTTGCAGCTGGAGAGGAGACTGCTCACATGTCACTTGTGGACAAGTCAGATCTCTTT TTTCATGATTATTCAATAGCACCCCTCTTCGTCCAGGAAAATTACATACACGTGAAGCCTGTAGCAGCA GGGGGTGACATGAAAAAGCACCTGATGCTTTTAAGCAGAGCAGCAGACAGCATATGCGATGGTGACCTA GTGGACAGCCAGATCCGGAGTAAGCAAAACTGGAGTCTTCTGCCTGCGCAGGCCATTTATGCCAGTGTT CTTCCTGGAGAGTTGATGAGGGGGTACATGACCCAGTTTCCCACCTTCCCAAGCTGGCTGGGGAAGCAC TCGTCTACAGGCAAACATGATCGTATTGTTCAGGACCTGGCCTTGCATATGAGTCTCAGAACTTACTCC AGCAAAAGGACTGTAAACATGGATTATCTGTCGCTTCTAAGGGATGCACTTGTACAGCCCTTGACCTCA CAAGGAGTAGACGGAGTACAGGATGTTGTTGCACTTATGGACACATATTATTTGATGAAAGAAGACTTT GAGAATATCATGGAAATCAGCAGCTGGGGTGGCAAACCTAGTCCCTTTTCAAAGCTGGATCCCAAGGTG AAAGCAGCCTTCACAAGAGCTTACAATAAGGAAGCCCACCTTACTCCATACTCACTTCAAGCTATAAAG GCATCTAGACACAGCACAAGCCCATCCCTGGATTCGGAATACAATGAAGAATTAAATGAAGATGACTCT CAATCTGATGAGAAAGACCAAGATGCTATAGAAACTGATGCCATGATCAAGAAAAAGACAAAATCTTCA AAGCCTTCAAAACCAGAAAAAGATAAGGAGCCCAGAAAAGGAAAAGGAAAAAGTTCGAAGAAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204747
Insert Size	3447 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204747.1
RefSeq Size	4899 bp
RefSeq ORF	3447 bp
Locus ID	5981
UniProt ID	P35251
Protein Families	Transcription Factors
Protein Pathways	DNA replication, Mismatch repair, Nucleotide excision repair
MW	128.3 kDa
Gene Summary	This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011] Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1, resulting in an isoform (2) that is 1 aa longer than isoform 1.

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