P2X5 (P2RX5) (NM_001204520) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LRH-1; P2X5; P2X5R
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331602 representing NM_001204520. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGCAGGCGGGCTGCAAGGGGCTCTGCCTGTCGCTGTTCGACTACAAGACCGAGAAGTATGTCATC GCCAAGAACAAGAAGGTGGGCCTGCTGTACCGGCTGCTGCAGGCCTCCATCCTGGCGTACCTGGTCGTA TGGGTGTTCCTGATAAAGAAGGGTTACCAAGACGTCGACACCTCCCTGCAGAGTGCTGTCATCACCAAA GTCAAGGGCGTGGCCTTCACCAACACCTCGGATCTTGGGCAGCGGATCTGGGATGTCGCCGACTACGTC ATTCCAGCCCAGAATGAAGGCATTCCTGATGGCGCGTGCTCCAAGGACAGCGACTGCCACGCTGGGGAA GCGGTTACAGCTGGAAACGGAGTGAAGACCGGCCGCTGCCTGCGGAGAGAGAACTTGGCCAGGGGCACC TGTGAGATCTTTGCCTGGTGCCCGTTGGAGACAAGCTCCAGGCCGGAGGAGCCATTCCTGAAGGAGGCC GAAGACTTCACCATTTTCATAAAGAACCACATCCGTTTCCCCAAATTCAACTTCTCCAAAAGCAATGTG ATGGACGTCAAGGACAGATCTTTCCTGAAATCATGCCACTTTGGCCCCAAGAACCACTACTGCCCCATC TTCCGACTGGGCTCCGTGATCCGCTGGGCCGGGAGCGACTTCCAGGATATAGCCCTGGAGGGTGGCGTG ATAGGAATTAATATTGAATGGAACTGTGATCTTGATAAAGCTGCCTCTGAGTGCCACCCTCACTATTCT TTTAGCCGTCTGGACAATAAACTTTCAAAGTCTGTCTCCTCCGGGTACAACTTCAGATTTGCCAGATAT TACCGAGACGCAGCCGGGGTGGAGTTCCGCACCCTGATGAAAGCCTACGGGATCCGCTTTGACGTGATG GTGAACGGCAAGGGTGCTTTCTTCTGCGACCTGGTACTCATCTACCTCATCAAAAAGAGAGAGTTTTAC CGTGACAAGAAGTACGAGGAAGTGAGGGGCCTAGAAGACAGTTCCCAGGAGGCCGAGGACGAGGCATCG GGGCTGGGGCTATCTGAGCAGCTCACATCTGGGCCAGGGCTGCTGGGGATGCCGGAGCAGCAGGAGCTG CAGGAGCCACCCGAGGCGAAGCGTGGAAGCAGCAGTCAGAAGGGGAACGGATCTGTGTGCCCACAGCTC CTGGAGCCCCACAGGAGCACGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204520
Insert Size	1197 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204520.1
RefSeq Size	2269 bp
RefSeq ORF	1197 bp
Locus ID	5026
UniProt ID	Q93086
Protein Families	Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
Protein Pathways	Calcium signaling pathway, Neuroactive ligand-receptor interaction
MW	44.5 kDa
Gene Summary	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream gene, TAX1BP3 (Tax1 binding protein 3). [provided by RefSeq, Mar 2011] Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (E) that is shorter than isoform A.

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