LIM Kinase 1 (LIMK1) (NM_001204426) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LIMK; LIMK-1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331586 representing NM_001204426. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTGTTGGCTTCAGCCCCAAGAAGACGCCGCTTCCTCCAGAGGGCTAAGTGTTGTGACTGCAGTGCC TCCCTGTCGCACCAGTACTATGAGAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCGCTAT GGCGAGTCCTGCCATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTGGCTGGGGAGCTGAAG TACCACCCCGAGTGTTTCATCTGCCTCACGTGTGGGACCTTTATCGGTGACGGGGACACCTACACGCTG GTGGAGCACTCCAAGCTGTACTGCGGGCACTGCTACTACCAGACTGTGGTGACCCCCGTCATCGAGCAG ATCCTGCCTGACTCCCCTGGCTCCCACCTGCCCCACACCGTCACCCTGGTGTCCATCCCAGCCTCATCT CATGGCAAGCGTGGACTTTCAGTCTCCATTGACCCCCCGCACGGCCCACCGGGCTGTGGCACCGAGCAC TCACACACCGTCCGCGTCCAGGGAGTGGATCCGGGCTGCATGAGCCCAGATGTGAAGAATTCCATCCAC GTCGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCCGAAATGTGCCCCTGGACGAGATTGACCTG CTGATTCAGGAAACCAGCCGCCTGCTCCAGCTGACCCTCGAGCATGACCCTCACGATACACTGGGCCAC GGGCTGGGGCCTGAGACCAGCCCCCTGAGCTCTCCGGCTTATACTCCCAGCGGGGAGGCGGGCAGCTCT GCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGCATCGACAGGTCTCCGGGCGCTGGCTCACTGGGCTCC CCGGCCTCCCAGCGCAAGGACCTGGGTCGCTCTGAGTCCCTCCGCGTAGTCTGCCGGCCACACCGCATC TTCCGGCCGTCGGACCTCATCCACGGGGAGGTGCTGGGCAAGGGCTGCTTCGGCCAGGCTATCAAGGTG ACACACCGTGAGACAGGTGAGGTGATGGTGATGAAGGAGCTGATCCGGTTCGACGAGGAGACCCAGAGG ACGTTCCTCAAGGAGGTGAAGGTCATGCGATGCCTGGAACACCCCAACGTGCTCAAGTTCATCGGGGTG CTCTACAAGGACAAGAGGCTCAACTTCATCACTGAGTACATCAAGGGCGGCACGCTCCGGGGCATCATC AAGAGCATGGACAGCCAGTACCCATGGAGCCAGAGAGTGAGCTTTGCCAAGGACATCGCATCAGGGATG GCCTACCTCCACTCCATGAACATCATCCACCGAGACCTCAACTCCCACAACTGCCTGGTCCGCGAGAAC AAGAATGTGGTGGTGGCTGACTTCGGGCTGGCGCGTCTCATGGTGGACGAGAAGACTCAGCCTGAGGGC CTGCGGAGCCTCAAGAAGCCAGACCGCAAGAAGCGCTACACCGTGGTGGGCAACCCCTACTGGATGGCA CCTGAGATGATCAACGGCCGCAGCTATGATGAGAAGGTGGATGTGTTCTCCTTTGGGATCGTCCTGTGC GAGATCATCGGGCGGGTGAACGCAGACCCTGACTACCTGCCCCGCACCATGGACTTTGGCCTCAACGTG CGAGGATTCCTGGACCGCTACTGCCCCCCAAACTGCCCCCCGAGCTTCTTCCCCATCACCGTGCGCTGT TGCGATCTGGACCCCGAGAAGAGGCCATCCTTTGTGAAGCTGGAACACTGGCTGGAGACCCTCCGCATG CACCTGGCCGGCCACCTGCCACTGGGCCCACAGCTGGAGCAGCTGGACAGAGGTTTCTGGGAGACCTAC CGGCGCGGCGAGAGCGGACTGCCTGCCCACCCTGAGGTCCCCGACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001204426
Insert Size	1842 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204426.1
RefSeq Size	3176 bp
RefSeq ORF	1842 bp
Locus ID	3984
UniProt ID	P53667
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
MW	68.7 kDa
Gene Summary	There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) lacks multiple exons at the 5' end and uses a different start codon, compared to variant 1. This variant encodes an isoform (2) with a shorter and distinct N-terminus that lacks one of two LIM zinc-binding domains, compared to isoform 1.

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