TTYH1 (NM_001201461) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331427 representing NM_001201461. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGGCGCCCCCGGGCTACCGGCCCTCAGCTTGGGTGCATCTCCTCCACCAGCTGCCCCGCGCCGAC TTCCAGCTCCGCCCGGTGCCCAGCGTTTTCGCGCCCCAAGAGCAGGAATACCAGCAGGCCTTGTTGCTG GTGGCGGCCTTGGCGGGCCTGGGCTTGGGCCTGAGCCTCATTTTCATCGCTGTCTACCTCATCCGCTTC TGCTGCTGCCGGCCCCCCGAGCCCCCCGGGTCCAAGATCCCCTCGCCCGGGGGAGGCTGCGTCACCTGG AGCTGCATTGTCGCCCTTCTCGCCGGCTGCACTGGCATTGGCATCGGTTTCTATGGCAACAGTGAGACC AGTGATGGGGTGTCCCAGCTCAGCTCTGCGCTGCTGCACGCCAACCACACACTCAGCACCATTGACCAC CTGGTGTTGGAGACGGTGGAGAGGCTGGGCGAGGCGGTGAGGACAGAGCTGACCACCCTGGAGGAGGTG CTCGAGCCGCGCACGGAGCTGGTGGCTGCCGCCCGAGGGGCTCGACGGCAGGCGGAGGCTGCGGCCCAG CAGCTGCAGGGGCTGGCCTTCTGGCAGGGAGTGCCCCTGAGCCCCCTGCAGGTGGCTGAAAATGTGTCC TTTGTGGAGGAGTACAGGTGGCTGGCCTACGTCCTCCTGCTGCTCCTGGAGCTGCTGGTCTGCCTCTTC ACCCTCCTGGGCCTGGCGAAGCAGAGCAAGTGGCTGGTGATCGTGATGACAGTCATGAGTCTCCTGGTT CTCGTCCTGAGCTGGGGCTCCATGGGCCTGGAGGCAGCCACGGCCGTGGGCCTCAGTGACTTCTGCTCC AATCCAGACCCTTATGTTCTGAACCTGACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTGAGCTAT TATCTCCTCTGCAACCGGGCCGTCTCCAACCCCTTCCAACAGAGGCTGACTCTGTCCCAGCGAGCTCTG GCCAACATCCACTCCCAGCTGCTGGGCCTGGAGCGAGAAGCTGTGCCTCAGTTCCCTTCAGCGCAGAAG CCTCTGCTGTCCTTGGAGGAGACTCTGAATGTGACAGAAGGAAATTTCCACCAGTTGGTGGCACTGCTA CACTGCCGCAGCCTGCACAAGGACTATGGTGCAGCCCTGCGGGGCCTGTGCGAAGACGCCCTGGAAGGC CTGCTCTTCCTGCTACTCTTCTCCCTGCTGTCTGCAGGAGCGCTGGCCACTGCCCTCTGCAGCCTGCCC CGAGCCTGGGCCCTCTTCCCACCCAGTGACGACTACGATGACACAGACGATGACGACCCTTTCAACCCT CAGCAGGAATCCAAGCGCTTTGTGCAGTGGCAGTCGTCTATCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001201461
Insert Size	1356 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201461.1
RefSeq Size	1924 bp
RefSeq ORF	1356 bp
Locus ID	57348
UniProt ID	Q9H313
Protein Families	Ion Channels: Other, Transmembrane
MW	49.2 kDa
Gene Summary	This gene encodes a member of the tweety family of proteins. Members of this family function as chloride anion channels. The encoded protein functions as a calcium(2+)-independent, volume-sensitive large conductance chloride(-) channel. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 5' end of an exon and contains an alternate exon compared to variant 2, that causes a frameshift. The resulting isoform (1) has a shorter and distinct C-terminus compared to isoform 2.

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