PTPN7 (NM_001199797) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BPTP-4; HEPTP; LC-PTP; LPTP; PTPNI
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331363 representing NM_001199797. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAACCGGCTGGGCTGCTGGCCCGCAAGGAGCCTGCCAAGGTCAGGCAGGGGCAGTGGGCAGAGGAGT CTTGGACTTGGGGGCCACCCTTCAGGTCCCAGGCATCCAGGCACCCCAGCAGCGAAGAGGACATGGGAG TCCCCTGGTGTGAGGGCCAGAGACTGTCCACTGTCTACCTCATGGCTGAGTGAGCCTCCCCTGGGCCCA GCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCGCTCCAGAGCACAGCCGTTGACCTTGTCTTTG GGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCAGCCAAGAAGCATGTGCGACTGCAGGAGAGG CGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCCTGGGGGCCGTAGAACCCATCTGCTCTGTG AACACACCCCGGGAGGTCACCCTACACTTTCTGCGCACTGCTGGACACCCCCTTACCCGCTGGGCCCTT CAGCGCCAGCCACCCAGCCCCAAGCAACTGGAAGAAGAATTCTTGAAGATCCCTTCAAACTTTGTCAGC CCCGAAGACCTGGACATCCCTGGCCACGCCTCCAAGGACCGATACAAGACCATCTTGCCAAATCCCCAG AGCCGTGTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGATTACATCAATGCCAACTACATCCGA GGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCCATGCCCAACACTGTGTCGGACTTC TGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCACTCAGCTCCGAGAGGGCAAGGAG AAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTATGGACCCTTCCAGATCCGCATCCAGGACATG AAAGAGTGCCCAGAATACACTGTGCGGCAGCTCACCATCCAGTACCAGGAAGAGCGCCGGTCAGTAAAG CACATCCTCTTTTCGGCCTGGCCAGACCATCAGACACCAGAATCAGCTGGGCCCCTGCTGCGCCTAGTG GCAGAGGTGGAGGAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGTAGTCCACTGCAGTGCAGGG ATTGGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTGAAAGCCCGAGGAGAAGTG GACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCCAGACGGCAGAGCAGTAC CAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCTGAGGAACCCAGCCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199797
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199797.1
RefSeq Size	2926 bp
RefSeq ORF	1305 bp
Locus ID	5778
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	MAPK signaling pathway
MW	48.3 kDa
Gene Summary	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (3) lacks a segment in the 5' region, resulting in an upstream AUG start codon, as compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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