AE2 (SLC4A2) (NM_001199694) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AE2; BND3L; EPB3L1; HKB3; NBND3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331346 representing NM_001199694. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGACTCAGCCAGAGCCAGAGAGCTTGGGCCCTGGGACGCCTGGGTTCCCCGAGCAGGAGGAAGACGAA CTTCACCGCACCCTGGGCGTGGAGCGGTTTGAGGAGATCCTACAGGAGGCCGGGTCTCGTGGAGGGGAG GAGCCAGGCCGCAGCTATGGGGAGGAAGACTTTGAGTACCACCGCCAGTCCTCCCACCACATCCATCAC CCACTGTCCACCCACCTGCCTCCGGATGCACGCCGCCGCAAGACACCCCAGGGCCCAGGACGGAAGCCT CGAAGGCGCCCGGGAGCCTCCCCGACTGGAGAAACCCCGACCATTGAGGAGGGGGAGGAAGATGAGGAT GAGGCCAGCGAGGCTGAGGGGGCCCGGGCTCTCACTCAGCCGTCCCCTGTCTCCACACCCTCCTCGGTG CAGTTCTTTCTCCAAGAGGATGACAGTGCTGACCGGAAGGCAGAGAGGACCAGTCCATCTTCCCCTGCA CCACTGCCCCACCAGGAGGCGACTCCTCGGGCCTCCAAAGGGGCCCAGGCTGGAACCCAGGTGGAGGAG GCGGAGGCGGAGGCGGTGGCGGTGGCCAGTGGCACTGCAGGGGGTGACGACGGGGGTGCCTCGGGGCGC CCCCTGCCCAAAGCCCAGCCTGGGCACCGCAGCTACAACCTTCAGGAGAGGAGGCGCATCGGGAGCATG ACTGGGGCTGAGCAGGCACTGCTGCCCCGGGTCCCCACGGATGAGATTGAGGCCCAGACGCTGGCCACG GCCGACCTAGACCTCATGAAGAGTCACCGGTTTGAGGACGTTCCTGGGGTGCGGCGGCACTTGGTGCGG AAGAATGCCAAAGGTTCCACACAGAGTGGCCGAGAAGGGCGGGAGCCTGGCCCCACACCTCGGGCCCGA CCCCGGGCCCCCCACAAGCCCCATGAGGTGTTTGTGGAGCTGAATGAGTTGCTCCTGGACAAAAACCAG GAGCCCCAGTGGCGGGAGACAGCTCGCTGGATCAAATTTGAAGAGGACGTGGAGGAGGAGACTGAGCGC TGGGGGAAGCCCCACGTGGCCTCCCTCTCCTTCCGCAGTCTCCTGGAGCTCCGCAGGACCCTGGCCCAT GGGGCTGTGCTCTTGGATCTGGACCAGCAGACCCTGCCCGGAGTGGCCCACCAGGTGGTGGAGCAGATG GTCATCTCTGACCAGATCAAGGCCGAGGACAGGGCCAACGTGCTGCGGGCTCTGCTGTTGAAACACAGC CACCCAAGTGATGAGAAGGACTTCTCCTTCCCCCGCAACATCTCAGCTGGCTCCCTGGGCTCCCTGCTG GGGCATCACCATGGTCAGGGGGCTGAGAGTGACCCCCACGTCACCGAGCCTCTCATGGGAGGTGTTCCT GAGACCCGGCTGGAGGTGGAGCGAGAGCGTGAGCTGCCGCCTCCAGCACCACCAGCTGGCATCACCCGC TCCAAGTCCAAGCACGAGCTGAAACTGCTGGAGAAGATTCCTGAGAATGCCGAGGCCACGGTGGTCCTT GTGGGCTGCGTGGAGTTCCTCTCCCGCCCCACCATGGCCTTTGTGCGGCTCCGGGAGGCTGTGGAGTTG GACGCAGTGTTGGAGGTGCCGGTGCCTGTGCGTTTCCTCTTCCTGCTGCTGGGCCCGAGTAGTGCCAAC ATGGACTACCACGAGATCGGCCGCTCCATCTCCACCCTCATGTCAGACAAGCAATTCCACGAGGCAGCC TACCTGGCTGACGAGCGGGAGGACCTGCTGACGGCCATCAACGCCTTCCTGGACTGCAGCGTGGTGCTG CCGCCTTCAGAAGTGCAGGGCGAGGAGCTGCTGCGCTCTGTGGCCCACTTCCAGCGCCAGATGCTCAAG AAGCGAGAGGAGCAGGGCCGGCTGCTACCTACAGGGGCTGGGCTGGAGCCCAAATCTGCCCAAGATAAG GCGCTCCTGCAGATGGTAGAGGCGGCAGGGGCAGCTGAAGATGATCCCCTTCGGCGGACGGGGCGGCCC TTTGGGGGGCTGATCCGAGATGTGCGGCGCCGCTATCCCCACTACCTGAGTGACTTCCGAGATGCACTT GACCCTCAGTGCCTGGCCGCAGTCATCTTCATCTACTTTGCCGCCCTGTCTCCTGCCATCACCTTTGGG GGGCTGCTGGGAGAGAAGACGCAGGACCTGATAGGGGTGTCGGAGCTGATTATGTCCACAGCGCTCCAG GGCGTGGTCTTCTGCCTGCTGGGTGCCCAGCCCCTGTTGGTGATCGGCTTCTCAGGGCCCCTGCTGGTC TTTGAGGAGGCCTTCTTCTCGTTCTGTAGCAGCAACCACCTGGAGTACCTGGTGGGCCGTGTGTGGATC GGCTTCTGGCTGGTGTTCCTGGCCCTGCTCATGGTGGCCCTGGAGGGGAGCTTCCTGGTCCGCTTCGTC TCCCGCTTCACCCAGGAGATCTTCGCCTTCTTGATCTCACTCATCTTCATCTATGAGACCTTCTACAAG CTGGTGAAGATCTTCCAGGAGCACCCCCTGCATGGCTGCTCAGCCTCCAACAGCTCAGAGGTGGACGGC GGTGAGAACATGACATGGGCCGGGGCAAGACCCACGCTGGGGCCGGGCAACAGGAGCTTGGCTGGGCAG TCTGGGCAGGGGAAGCCCCGGGGCCAGCCCAACACGGCCCTGCTGTCGCTGGTGCTCATGGCCGGCACC TTCTTCATCGCCTTCTTCCTGCGCAAATTCAAGAACAGCCGGTTCTTTCCTGGCCGGATCCGGCGGGTG ATTGGGGACTTTGGGGTGCCCATCGCCATCCTCATCATGGTGCTTGTGGATTACAGTATTGAGGACACC TATACCCAGAAGCTGAGCGTTCCCAGTGGATTCTCGGTGACTGCCCCAGAAAAGAGGGGCTGGGTCATC AACCCCCTGGGAGAGAAGAGCCCCTTCCCTGTGTGGATGATGGTTGCCAGCCTGCTGCCCGCCATCCTG GTCTTCATTCTCATCTTCATGGAGACACAGATCACCACGCTCATCATCTCCAAGAAGGAGCGCATGCTG CAGAAGGGCTCCGGCTTCCACCTGGACCTGCTGCTCATCGTGGCCATGGGCGGCATCTGTGCCCTCTTT GGCCTGCCCTGGTTGGCTGCTGCCACTGTCCGCTCTGTCACTCACGCCAACGCGCTCACTGTCATGAGC AAGGCTGTGGCACCTGGGGACAAGCCCAAGATTCAGGAAGTCAAGGAGCAGCGGGTGACGGGGCTGCTG GTTGCCCTGCTTGTGGGCCTCTCCATAGTTATCGGGGATCTGCTCCGGCAGATCCCCCTGGCCGTGCTC TTTGGAATTTTCCTGTACATGGGAGTCACCTCCCTTAACGGGATCCAGTTCTATGAGCGGCTGCATCTG CTGCTCATGCCGCCCAAACACCACCCAGATGTCACTTACGTCAAGAAGGTCCGGACCCTCCGTATGCAC CTGTTCACGGCCCTGCAGCTGCTCTGCCTGGCCCTGCTCTGGGCCGTCATGTCCACAGCTGCCTCCCTG GCCTTCCCCTTCATCCTCATCCTCACAGTGCCGCTCCGCATGGTGGTGCTCACCCGTATCTTCACCGAC CGAGAGATGAAATGTCTGGATGCTAACGAGGCAGAGCCGGTGTTTGATGAGCGGGAGGGTGTGGACGAG TACAATGAGATGCCCATGCCTGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001199694
Insert Size	3684 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199694.1
RefSeq Size	4390 bp
RefSeq ORF	3684 bp
Locus ID	6522
UniProt ID	P04920
Protein Families	Transmembrane
MW	135.6 kDa
Gene Summary	This gene encodes a member of the anion exchanger family of membrane transport proteins. The encoded protein regulates intracellular pH, biliary bicarbonate secretion, and chloride uptake. Reduced expression of this gene may be associated with primary biliary cirrhosis (PBC) in human patients, while differential expression of this gene may be associated with malignant hepatocellular carcinoma, colon and gastric cancers. [provided by RefSeq, Nov 2016] Transcript Variant: This variant (4) lacks two exons from the 5' end and contains an alternate 5' exon, as compared to variant 1. The resulting isoform (3) has a shorter and different N-terminus, as compared to isoform 1.

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