A1CF (NM_001198818) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACF; ACF64; ACF65; APOBEC1CF; ASP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331225 representing NM_001198818. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAATCAAATCACAAATCCGGGGATGGATTGAGCGGCACTCAGAAGGAAGCAGCCCTCCGCGCACTG GTCCAGCGCACAGGATATAGCTTGGTCCAGGAAAATGGACAAAGAAAATATGGTGGCCCTCCACCTGGT TGGGATGCTGCACCCCCTGAAAGGGGCTGTGAAATTTTTATTGGAAAACTTCCCCGAGACCTTTTTGAG GATGAGCTTATACCATTATGTGAAAAAATCGGTAAAATTTATGAAATGAGAATGATGATGGATTTTAAT GGCAACAATAGAGGATATGCATTTGTAACATTTTCAAATAAAGTGGAAGCCAAGAATGCAATCAAGCAA CTTAATAATTATGAAATTAGAAATGGGCGCCTCTTAGGGGTTTGTGCCAGTGTGGACAACTGCCGATTA TTTGTTGGGGGCATCCCAAAAACCAAAAAGAGAGAAGAAATCTTATCGGAGATGAAAAAGGTTACTGAA GGTGTTGTCGATGTCATCGTCTACCCAAGCGCTGCAGATAAAACCAAAAACCGAGGCTTTGCCTTCGTG GAGTATGAGAGTCATCGAGCAGCTGCCATGGCGAGGAGGAAACTGCTACCAGGAAGAATTCAGTTATGG GGACATGGTATTGCAGTAGACTGGGCAGAGCCAGAAGTAGAAGTTGATGAAGATACAATGTCTTCAGTG AAAATCCTATATGTAAGAAATCTTATGCTGTCTACCTCTGAAGAGATGATTGAAAAGGAATTCAACAAT ATCAAACCAGGTGCTGTGGAGAGGGTGAAGAAAATTCGAGACTATGCTTTTGTGCACTTCAGTAACCGA GAAGATGCAGTTGAGGCTATGAAAGCTTTAAATGGCAAGGTGCTGGATGGTTCCCCCATTGAAGTCACC CTAGCAAAACCAGTGGACAAGGACAGTTATGTTAGGTATACCCGAGGCACAGGTGGAAGGGGCACCATG CTGCAAGGAGAGTATACCTACTCTTTGGGCCAAGTTTATGATCCCACCACAACCTACCTTGGAGCTCCT GTCTTCTATGCCCCCCAGACCTATGCAGCAATTCCCAGTCTTCATTTCCCAGCCACCAAAGGACATCTC AGCAACAGAGCCATTATCCGAGCCCCTTCTGTTAGAGGGGCTGCGGGAGTGAGAGGACTGGGCGGCCGT GGCTATTTGGCATACACAGGCCTGGGTCGAGGATACCAGGTCAAAGGAGACAAAAGAGAAGACAAACTC TATGACATTTTACCTGGGATGGAGCTCACCCCAATGAATCCTGTCACATTAAAACCCCAAGGAATTAAA CTCGCTCCCCAGATATTAGAAGAGATTTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAGCTGCAC TCTGCTATTGGACAAGACCAAAGACAGCTATTCTTGTACAAAATAACTATTCCTGCTCTAGCCAGCCAG AATCCTGCAATCCACCCTTTCACACCTCCAAAGCTGAGTGCCTTTGTGGATGAAGCAAAGACGTATGCA GCCGAATACACCCTGCAGACCCTGGGCATCCCCACTGATGGAGGCGATGGCACCATGGCTACTGCTGCT GCTGCTGCTACTGCTTTCCCAGGATATGCTGTCCCTAATGCAACTGCACCCGTGTCTGCAGCCCAGCTC AAGCAAGCGGTAACCCTTGGACAAGACTTAGCAGCATATACAACCTATGAGGTCTACCCAACTTTTGCA GTGACTGCCCGAGGGGATGGATATGGCACCTTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001198818
Insert Size	1761 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198818.1
RefSeq Size	9362 bp
RefSeq ORF	1761 bp
Locus ID	29974
UniProt ID	Q9NQ94
MW	64.3 kDa
Gene Summary	Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence and lacks an alternate in-frame segment compared to variant 5. The resulting isoform (1) has a shorter and distinct N-terminus and lacks a short internal segment compared to isoform 4. Variants 1, 4, and 7 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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