RBM15 (NM_001201545) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	OTT; OTT1; SPEN
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331110 representing NM_001201545. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGACTGCGGGGCGGGACCCTGTGCCGCGGCGGAGTCCAAGATGGCGGCGTGCGGTTCCGCTGTGT GAAACGAGCGCGGGGCGGCGGGTTACTCAGCTCCGCGGAGACGACCTCCGACGACCCGCAACAATGAAG GGAAAAGAGCGCTCGCCAGTGAAGGCCAAACGCTCCCGTGGTGGTGAGGACTCGACTTCCCGCGGTGAG CGGAGCAAGAAGTTAGGGGGCTCTGGTGGCAGCAATGGGAGCAGCAGCGGAAAGACCGATAGCGGCGGT GGGTCGCGGCGGAGTCTCCACCTGGACAAGTCCAGCAGTCGAGGTGGCAGCCGCGAGTATGATACCGGT GGGGGCAGCTCCAGTAGCCGCTTGCATAGTTATAGCTCCCCGAGCACCAAAAATTCTTCGGGCGGGGGC GAGTCGCGCAGCAGCTCCCGGGGTGGAGGCGGGGAGTCACGTTCCTCTGGGGCCGCCTCCTCAGCTCCC GGCGGCGGGGACGGCGCGGAATACAAGACTCTGAAGATAAGCGAGTTGGGGTCCCAGCTTAGTGACGAA GCGGTGGAGGACGGCCTGTTTCATGAGTTCAAACGCTTCGGTGATGTAAGTGTGAAAATCAGTCATCTG TCGGGTTCTGGCAGCGGGGATGAGCGGGTAGCCTTTGTGAACTTCCGGCGGCCAGAGGACGCGCGGGCG GCCAAGCATGCCAGAGGCCGCCTGGTGCTCTATGACCGGCCTCTGAAGATAGAAGCTGTGTATGTGAGC CGGCGCCGCAGCCGCTCCCCTTTAGACAAAGATACTTATCCTCCATCAGCCAGTGTGGTCGGGGCCTCT GTAGGTGGTCACCGGCACCCCCCTGGAGGTGGTGGAGGCCAGAGATCACTTTCCCCTGGTGGCGCTGCT TTGGGATACAGAGACTACCGGCTGCAGCAGTTGGCTCTTGGCCGCCTGCCCCCTCCACCTCCGCCACCA TTGCCTCGAGACCTGGAGAGAGAAAGAGACTACCCGTTCTATGAGAGAGTGCGCCCTGCATACAGTCTT GAGCCAAGGGTGGGAGCTGGAGCAGGTGCTGCTCCTTTCAGAGAAGTGGATGAGATTTCACCCGAGGAT GATCAGCGAGCTAACCGGACGCTCTTCTTGGGCAACCTAGACATCACTGTAACGGAGAGTGATTTAAGA AGGGCGTTTGATCGCTTTGGAGTCATCACAGAAGTAGATATCAAGAGGCCTTCTCGCGGCCAGACTAGT ACTTACGGCTTTCTCAAATTTGAGAACTTAGATATGTCTCACCGGGCCAAATTAGCAATGTCTGGCAAA ATTATAATTCGGAATCCTATCAAAATTGGTTATGGTAAAGCTACACCCACCACCCGCCTCTGGGTGGGA GGCCTGGGACCTTGGGTTCCTCTTGCTGCCCTGGCACGAGAATTTGATCGATTTGGCACCATACGCACC ATAGACTACCGAAAAGGTGATAGTTGGGCATATATCCAGTATGAAAGCCTGGATGCAGCGCATGCTGCC TGGACCCATATGCGGGGCTTCCCACTTGGTGGCCCAGATCGACGCCTTAGAGTAGACTTTGCCGACACC GAACATCGTTACCAGCAGCAGTATCTGCAGCCTCTGCCCTTGACTCATTATGAGCTGGTGACAGATGCT TTTGGACATCGGGCACCAGACCCTTTGAGGGGTGCTCGGGATAGGACACCACCCTTACTATACAGAGAT CGTGATAGGGACCTTTATCCTGACTCTGATTGGGTGCCACCCCCACCCCCAGTCCGAGAACGCAGCACT CGGACTGCAGCTACTTCTGTGCCTGCTTACGAGCCACTGGATAGCCTAGATCGCAGGCGGGATGGTTGG TCCTTGGACCGGGACAGAGGTGATCGAGATCTGCCCAGCAGCAGAGACCAGCCTAGGAAGCGAAGGCTG CCTGAGGAGAGTGGAGGACGTCATCTGGATAGGTCTCCTGAGAGTGACCGCCCACGAAAACGTCACTGC GCTCCTTCTCCTGACCGCAGTCCAGAATTGAGCAGTAGCCGGGATCGTTACAACAGCGACAATGATCGA TCTTCCCGTCTTCTCTTGGAAAGGCCCTCTCCAATCAGAGACAGACGAGGTAGTTTGGAGAAGAGCCAG GGTGACAAGCGAGACCGTAAAAACTCTGCATCAGCTGAACGAGATAGGAAGCACCGGACAACTGCTCCC ACTGAGGGAAAAAGCCCTCTGAAAAAAGAAGACCGCTCTGATGGGAGTGCACCTAGCACCAGCACTGCT TCCTCCAAGCTGAAGTCCCCGTCCCAGAAACAGGATGGGGGGACAGCCCCTGTGGCATCAGCCTCTCCC AAACTCTGTTTGGCCTGGCAGGGCATGCTTCTACTGAAGAACAGCAACTTTCCTTCCAACATGCATCTG TTGCAGGGTGACCTCCAAGTGGCTAGTAGTCTTCTTGTGGAGGGTTCAACTGGAGGCAAAGTGGCCCAG CTCAAGATCACTCAGCGTCTCCGTTTGGACCAGCCCAAGTTGGATGAAGTAACTCGACGCATCAAAGTA GCAGGGCCCAATGGTTATGCCATTCTTTTGGCTGTGCCTGGAAGTTCTGACAGCCGGTCCTCCTCTTCC TCAGCTGCATCAGACACTGCCACTTCTACTCAGAGGCCACTTAGGAACCTTGTGTCCTATTTAAAGCAA AAGCAGGCAGCCGGGGTGATCAGCCTCCCTGTGGGGGGCAACAAAGACAAGGAAAACACCGGGGTCCTT CATGCCTTCCCACCTTGTGAGTTCTCCCAGCAGTTCCTGGATTCCCCTGCCAAGGCACTGGCCAAATCT GAAGAAGATTACCTGGTCATGATCATTGTCCGTGCAAAACTGGTTGAACAGCGGATGAAGATATGGAAT TCAAAGCTCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001201545
Insert Size	2910 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201545.1
RefSeq Size	3338 bp
RefSeq ORF	2910 bp
Locus ID	64783
UniProt ID	Q96T37
Protein Families	Transcription Factors
MW	106.4 kDa
Gene Summary	Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM, Feb 2009] Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1.

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