ABHD10 (NM_001272069) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331032 representing NM_001272069. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGTTGCGCGCTTGGCAGCTGTGGCGGCCTGGGTACCTTGTCGGAGCTGGGGCTGGGCAGCCGTC CCCTTCGGTCCCCACCGTGGCCTCAGCGTGCTGCTTGCACGGATACCTCAGCGGGCGCCACGGTGGCTC CCAGCTTGTAGACAAAAGACGTCACTCTCATTCCTTAATCGACCAGACCTTCCAAACCTGGCTTATAAG AAGCTAAAAGGCAAAAGTCCAGGAATTATCTTCATCCCTGGCTATCTTTCTTATATGAATGGTACAAAA GCGTTGGCGATTGAGGAGTTTTGCAAATCTCTAGGTCACGCCTGCATAAGGTTTGATTACTCAGGAGTT GGAAGTTCAGATGGTAACTCAGAGGAAAGCACACTGGGGAAATGGAGAAAAGATGTTCTTTCTATAATT GATGACTTAGCTGATGGGCCACAGATTCTTGTTGGATCTAGCCTTGGAGGGTGGCTTATGCTTCATGCT GCAATTGCACGACCAGAGAAGGTCGTGGCTCTTATTGGTGTAGCTACAGCTGCAGATACCTTAGTGACA AAGTTTAATCAGCTTCCTGTTGAGGACTCTGGAAGGAAAAACTATATTTCTTTACATTCAGCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001272069
Insert Size	618 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272069.1
RefSeq Size	2823 bp
RefSeq ORF	618 bp
Locus ID	55347
UniProt ID	Q9NUJ1
MW	22.1 kDa
Gene Summary	This gene encodes a mitochondrially-localized enzyme that acts in liver cells as a hydrolase. The encoded protein removes glucuronide from mycophenolic acid acyl-glucuronide. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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