CEP76 (NM_001271989) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C18orf9; HsT1705
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331018 representing NM_001271989. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCGCTGCCTCCGGAGAAAGCCTCCGAGCTGAAGCAGCTCATCCACCAGCAGCTGAGCAAGATGGAT GTCCATGGTAGAATAAGAGAAATCCTTGCTGAGACTATACGGGAAGAATTGGCACCTGATCAACAGCAT TTATCAACAGAAGATTTGATCAAAGCCCTTAGACGTCGAGGAATCATTGACGATGTGATGAAAGAACTT AATTTTGTTACTGACAGTGTTGAGCAAGAACTCCCTTCCTCTCCAAAACAACCTATTTGTTTTGATAGA CAATCGACATTAAAAAAAAGTGATGGAACTAGAATGGCTGATTCAACAACAATGTTATCAATAAGTGAT CCAATTCATATGGTGCTAATCAAAACAGACATATTTGGTGAGACGACTTTAGTAGCATCATATTTTCTG GAATGGCGATCGGTTTTGGGCTCAGAAAATGGAGTGACCAGTCTGACTGTGGAACTTATGGGTGTAGGC ACAGAATCAAAAGTTTCTGTGGGAATTTTAAATATAAAACTTGAAATGTATCCACCACTCAATCAAACG TTATCTCAAGAAGTAGTGAACACACAGCTTGCTTTGGAACGTCAGAAAACTGCAGAGAAAGAGCGATTA TTTCTTGTATATGCTAAGCAGTGGTGGAGAGAATATTTGCAAATTCGACCCTCACACAACTCACGACTG GTTAAGATTTTTGCACAGGATGAAAATGGGATAAATAGACCAGTCTGTTCCTATGTTAAACCACTTCGA GCTGGACGGCTTCTTGATACTCCAAGGCAAGCAGCAAGATTTGTTAATGTCCTTGGTTATGAACGAGCC CCTGTTATTGGAGGAGGAGGTAAACAGGAGCAGTGGTGCACTCTGCTGGCCTTTCTCTGTAGAAACAAG GGTGACTGTGAAGATCACGCTAACCTTCTGTGCAGCCTTCTTCTTGGATATGGATTAGAAGCCTTTGTT TGTGTTGGGACCAAGGCAAAAGGAGTACCTCATGCATGGGTTATGACTTGTGGAACTGATGGGGCCATC ACTTTTTGGGAGAGTTTAACAGGACACAGGTACATCCATAAACCTACCAATCCTGATGAACCTCCAGTT GCTGAACAGCCCAAACCACTGTACCCATATCGAACAATTGGTTGTGTTTTCAACCATCAGATGTTCCTG GGAAATTGTCAACCCTCTGATGCAGTAGAAACCTGTGTATTTGATTTGAACGATGAATCCAAATGGAAA CCCATGAGTGAGGAAGCAATTAAATCTGTGTGTGCTCCTGGAGCTACAACATCCCTTCCTCCCTTTCCA CCTCTGTGTGCATCCACAATTGACGCGTCAGTAACAAGTAATGAAATTGAAATGCAGCTGAGGCTCCTG GTGTCAGAACACAGGAAGGATCTTGGCCTCACTACTGTTTGGGAAGACCAGCTCTCCTACCTTTTATCA CCAGCTTTGGCTTCTTATGAATTTGAGCGTACAACAAGTATATCAGCAGGCAATGAAGAATTTCAAGAT GCCATAAGAAGGGCTGTACCTGATGGTCACACATTTAAAGGGTTCCCAATACATTTTGTGTATAGAAAT GCAAGACGTGCATTTGCCACATGTCTTCGATCTCCTTTCTGTGAAGAAATAATCTGTTGCCGTGGAGAC CAAGTGCGACTGGCAGTTCGTGTCCGAGTATTTACTTACCCTGAATCTGCATGTGCTGTTTGGATCATG TTTGCTTGTAAATATCGCTCGGTATTATAG
Restriction Sites	SgfI-RsrII
ACCN	NM_001271989
Insert Size	1755 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271989.1
RefSeq Size	2722 bp
RefSeq ORF	1755 bp
Locus ID	79959
UniProt ID	Q8TAP6
MW	65.7 kDa
Gene Summary	This gene encodes a centrosomal protein which regulates centriole amplification by limiting centriole duplication to once per cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform (b) which is shorter than isoform a.

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