SEMA4F (NM_001271662) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	M-SEMA; m-Sema-M; PRO2353; S4F; SEMAM; SEMAW
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330951 representing NM_001271662. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGGCCTCTGCTGCGCGGCCCCGCCCGGGTCCCGGGCAGCCTACAGCCTCGCCCTTCCCGCTACTG CTGCTGGCGGTGCTGAGCGGCCCGGTATCCGGCCGCGTCCCCCGCTCGGTGCCCAGAACCTCGCTTCCA ATCTCTGAGGCTGACTCCTGTCTCACCCGGTTCGCAGTCCCTCACACATACAATTACTCTGTTCTCCTT GTGGATCCTGCCTCCCACACACTTTATGTTGGCGCCCGGGACACCATCTTCGCTTTATCCCTGCCCTTC TCAGGGGAGAGACCCCGCAGGATTGACTGGATGGTTCCTGAGGCTCACAGACAGAACTGTAGGAAGAAA GGCAAGAAAGAGGATGTGTCCAGGTTCCAGCAGGTTGAAAGACTTGAGAGTGGCCGGGGGAAATGTCCT TTTGAGCCAGCTCAGCGGTCAGCAGCTGTAATGGCTGGGGGGGTCCTCTATGCTGCCACTGTGAAAAAC TACCTGGGGACGGAGCCAATTATCACCAGAGCAGTGGGTCGTGCCGAGGACTGGATTCGGACAGATACC TTGCCTTCCTGGCTGAACGCCCCAGCCTTTGTCGCAGCCGTGGCCTTGAGCCCAGCCGAATGGGGGGAT GAAGATGGAGACGACGAAATCTACTTCTTCTTTACGGAGACTTCCCGAGCATTTGACTCATACGAGCGC ATTAAAGTCCCACGGGTGGCCCGTGTGTGTGCGGGGGACCTCGGGGGCCGGAAGACCCTCCAGCAGAGA TGGACGACGTTTTTGAAAGCTGACCTGCTCTGTCCAGGGCCTGAGCATGGCCGGGCCTCCAGTGTCCTG CAGGATGTTGCTGTGCTTCGACCTGAGCTTGGGGCAGGGACTCCCATCTTTTATGGCATCTTTTCTTCC CAGTGGGAGGGGGCTACTATCTCTGCTGTCTGTGCCTTCCGACCACAAGACATTCGGACAGTGCTGAAT GGTCCCTTCAGAGAACTAAAACATGACTGCAACAGAGGACTGCCTGTCGTGGACAATGATGTGCCCCAG CCCAGACCTGGAGAGTGCATCACCAACAACATGAAGCTCCGGCACTTTGGCTCATCTCTCTCCCTGCCT GACCGCGTACTCACCTTCATCCGGGACCACCCACTCATGGACAGGCCAGTGTTTCCAGCTGATGGCCAC CCCCTGCTGGTCACTACAGATACAGCCTATCTCAGAGTCGTGGCCCACAGGGTGACCAGCCTCTCAGGG AAAGAGTATGATGTGCTCTACCTGGGGACAGAGGATGGACACCTCCACCGAGCAGTGCGGATCGGAGCT CAGCTCAGCGTTCTTGAAGATCTGGCCTTATTCCCAGAGCCACAGCCAGTTGAGAACATGAAATTGTAC CACAGCTGGCTCCTGGTTGGCTCCCGTACTGAGGTGACACAAGTGAATACAACCAACTGTGGCCGTCTC CAGAGCTGCTCAGAGTGCATCCTGGCCCAGGACCCAGTCTGTGCCTGGAGCTTCCGGCTGGATGAGTGT GTGGCCCATGCCGGGGAGCACCGAGGGTTGGTCCAAGACATAGAGTCAGCAGATGTCTCCTCTTTGTGT CCTAAAGAGCCTGGAGAACGTCCAGTAGTGTTTGAAGTTCCCGTGGCTACAGCTGCGCATGTGGTCTTG CCATGTTCTCCAAGCTCAGCATGGGCATCCTGTGTGTGGCACCAGCCCAGTGGAGTGACTGCACTCACC CCCCGGCGGGATGGACTGGAGGTGGTGGTGACCCCAGGGGCCATGGGCGCTTATGCCTGTGAATGTCAG GAGGGTGGGGCAGCCCATGTGGTAGCAGCTTACAGCTTGGTATGGGGCAGCCAGCGAGATGCTCCGAGC CGGGCCCACACAGTGGGGGCGGGACTGGCTGGCTTCTTCTTGGGGATTCTCGCAGCATCCCTGACTCTC ATTCTGATTGGTCGGCGTCAGCAGCGACGGCGACAGAGGGAACTTCTGGCTAGAGACAAGGTGGGCCTG GACCTGGGGGCTCCACCTTCTGGGACCACAAGCTACAGCCAAGACCCTCCCTCCCCCTCTCCTGAAGAT GAGCGGTTGCCGCTGGCCCTGGCCAAGAGGGGCAGTGGCTTTGGTGGATTCTCACCACCCTTCCTGCTT GATCCTTGCCCAAGCCCAGCCCACATTCGGCTAACTGGGGCTCCTCTAGCCACATGTGATGAAACATCC ATCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271662
Insert Size	2214 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271662.1
RefSeq Size	6023 bp
RefSeq ORF	2214 bp
Locus ID	10505
UniProt ID	O95754
Protein Families	Transmembrane
Protein Pathways	Axon guidance
MW	80 kDa
Gene Summary	This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (3) lacks an internal in-frame exon in the coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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