NKD2 (NM_001271082) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Naked2
Vector	PCMV6-Neo
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001271082, the custom clone sequence may differ by one or more nucleotides ATGGGGAAACTGCAGTCGAAGCACGCCGCCGCCGCCCGCAAGCGGAGAGAGAGCCCGGAAGGGGACAGCT TCGTGGCGTCCGCGTACGCGAGCGGCCGCAAAGGCGCGGAGGAAGCGGAGCGGCGCGCGCGGGACAAGCA GGAGCTGCCCAATGGGGACCCCAAGGAGGGGCCTTTCCGGGAGGACCAGTGTCCCCTACAGGTGGCACTC CCCGCTGAGAAAGCTGAGGGCCGCGAGCACCCGGGACAACTCCTCAGCGCAGATGACGGAGAGAGGGCAG CAAACCGCGAGGGCCCGCGAGGACCGGGCGGGCAGCGCCTCAACATTGACGCACTCCAGTGCGATGTCTC GGTGGAGGAGGACGACCGCCAGGAGTGGACGTTCACGCTCTATGACTTTGACAACTGCGGGAAGGTCACC AGGGAGGACATGTCCAGCCTCATGCACACCATCTATGAGGTCGTGGATGCCTCGGTCAACCACTCCTCGG GCAGCAGCAAGACCCTCCGTGTGAAGCTAACCGTCAGCCCTGAGCCCTCCAGCAAGAGGAAGGAGGGTCC TCCTGCTGGCCAGGACCGGGAGCCCACCCGTTGCAGGATGGAGGGTGAACTGGCAGAGGAGCCAAGGGTG GCTGACAGGAGGTTGTCTGCACACGTCAGGAGGCCCAGTACTGACCCCCAGCCCTGCTCGGAGCGGGGGC CCTACTGCGTGGACGAGAACACGGAGCGCAGAAACCACTACCTGGACCTCGCCGGGATTGAGAACTACAC GTCCAGATTCGGCCCTGGGTCTCAGCTGTGCGAGAAGAGAAGCTCCGCTCCCAGGACACACAGTGGGGAC AAGGCTAGAGGAGTCGGCCTTTGCAGGGAGCTGTGGAGCCAGGCAGGTCACCCACAGTGGCCAGGCCCCT TCCCTTCAGGGCTGGTGGCCGTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271082
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271082.1, NP_001258011.1
RefSeq Size	2070 bp
RefSeq ORF	936 bp
Locus ID	85409
UniProt ID	Q969F2
Protein Families	Druggable Genome
Protein Pathways	Wnt signaling pathway
Gene Summary	This gene encodes a member of a family of proteins that function as negative regulators of Wnt receptor signaling through interaction with Dishevelled family members. The encoded protein participates in the delivery of transforming growth factor alpha-containing vesicles to the cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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