NKD2 (NM_001271082) Human Tagged ORF Clone
CAT#: RC232453
- TrueORF®
NKD2 (Myc-DDK tagged) - Homo sapiens naked cuticle homolog 2 (Drosophila) (NKD2), transcript variant 2
ORF Plasmid: tGFP
"NM_001271082" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Naked2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC232453 representing NM_001271082
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAACTGCAGTCGAAGCACGCCGCCGCCGCCCGCAAGCGGAGAGAGAGCCCGGAAGGGGACAGCT TCGTGGCGTCCGCGTACGCGAGCGGCCGCAAAGGCGCGGAGGAAGCGGAGCGGCGCGCGCGGGACAAGCA GGAGCTGCCCAATGGGGACCCCAAGGAGGGGCCTTTCCGGGAGGACCAGTGTCCCCTACAGGTGGCACTC CCCGCTGAGAAAGCTGAGGGCCGCGAGCACCCGGGACAACTCCTCAGCGCAGATGACGGAGAGAGGGCAG CAAACCGCGAGGGCCCGCGAGGACCGGGCGGGCAGCGCCTCAACATTGACGCACTCCAGTGCGATGTCTC GGTGGAGGAGGACGACCGCCAGGAGTGGACGTTCACGCTCTATGACTTTGACAACTGCGGGAAGGTCACC AGGGAGGACATGTCCAGCCTCATGCACACCATCTATGAGGTCGTGGATGCCTCGGTCAACCACTCCTCGG GCAGCAGCAAGACCCTCCGTGTGAAGCTAACCGTCAGCCCTGAGCCCTCCAGCAAGAGGAAGGAGGGTCC TCCTGCTGGCCAGGACCGGGAGCCCACCCGTTGCAGGATGGAGGGTGAACTGGCAGAGGAGCCAAGGGTG GCTGACAGGAGGTTGTCTGCACACGTCAGGAGGCCCAGTACTGACCCCCAGCCCTGCTCGGAGCGGGGGC CCTACTGCGTGGACGAGAACACGGAGCGCAGAAACCACTACCTGGACCTCGCCGGGATTGAGAACTACAC GTCCAGATTCGGCCCTGGGTCTCAGCTGTGCGAGAAGAGAAGCTCCGCTCCCAGGACACACAGTGGGGAC AAGGCTAGAGGAGTCGGCCTTTGCAGGGAGCTGTGGAGCCAGGCAGGTCACCCACAGTGGCCAGGCCCCT TCCCTTCAGGGCTGGTGGCCGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC232453 representing NM_001271082
Red=Cloning site Green=Tags(s) MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVAL PAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVT REDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRV ADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSQLCEKRSSAPRTHSGD KARGVGLCRELWSQAGHPQWPGPFPSGLVAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271082 |
ORF Size | 933 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271082.1, NP_001258011.1 |
RefSeq Size | 2070 bp |
RefSeq ORF | 936 bp |
Locus ID | 85409 |
UniProt ID | Q969F2 |
Protein Families | Druggable Genome |
Protein Pathways | Wnt signaling pathway |
MW | 34.8 kDa |
Gene Summary | This gene encodes a member of a family of proteins that function as negative regulators of Wnt receptor signaling through interaction with Dishevelled family members. The encoded protein participates in the delivery of transforming growth factor alpha-containing vesicles to the cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
Documents
Product Manuals |
FAQs |
SDS |