hHR23A (RAD23A) (NM_001270362) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HHR23A; HR23A
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330807 representing NM_001270362. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCGTCACCATCACGCTCAAAACGCTGCAGCAGCAGACCTTCAAGATCCGCATGGAGCCTGACGAG ACGGTGAAGGTGCTAAAGGAGAAGATAGAAGCTGAGAAGGGTCGTGATGCCTTCCCCGTGGCTGGACAG AAACTCATCTATGCCGGCAAGATCTTGAGTGACGATGTCCCTATCAGGGACTATCGCATCGATGAGAAG AACTTTGTGGTCGTCATGGTGACCAAGACCAAAGCCGGCCAGGGTACCTCAGCACCCCCAGAGGCCTCA CCCACAGCTGCCCCAGAGTCCTCTACATCCTTCCCGCCTGCCCCCACCTCAGGCATGTCCCATCCCCCA CCTGCCGCCAGAGAGGACAAGAGCCCATCAGAGGAATCCGCCCCCACGACGTCCCCAGAGTCTGTGTCA GGCTCTGTTCCCTCTTCAGGTAGCAGCGGGCGAGAGGAAGACGCGGCCTCCACGCTAGTGACGGGCTCT GAGTATGAGACGATGCTGACGGAGATCATGTCCATGGGCTATGAGCGAGAGCGGGTCGTGGCCGCCCTG AGAGCCAGCTACAACAACCCCCACCGAGCCGTGGAGTATCTGCTCACGGGAATTCCTGGGAGCCCCGAG CCGGAACACGGTTCTGTCCAGGAGAGCCAGGTATCGGAGCAGCCGGCCACGGAAGCAGGAGAGAACCCC CTGGAGTTCCTGCGGGACCAGCCCCAGTTCCAGAACATGCGGCAGGTGATTCAGCAGAACCCTGCGCTG CTGCCCGCCCTGCTCCAGCAGCTGGGCCAGGAGAACCCTCAGCTTTTACAGCAAATCAGCCGGCACCAG GAGCAGTTCATCCAGATGCTGAACGAGCCCCCTGGGGAGCTGGCGGACATCTCAGATGTGGAGGGGGAG GTGGGCGCCATAGGAGAGGAGGCCCCGCAGATGAACTACATCCAGGTGACGCCGCAGGAGAAAGAAGCT ATAGAGAGGTTGAAGGCCCTGGGCTTCCCAGAGAGCCTGGTCATCCAGGCCTATTTCGCGTGTGAAAAA AATGAGAACTTGGCTGCCAACTTCCTCCTGAGTCAGAACTTTGATGACGAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270362
Insert Size	1089 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270362.1
RefSeq Size	1818 bp
RefSeq ORF	1089 bp
Locus ID	5886
UniProt ID	P54725
Protein Families	Druggable Genome
Protein Pathways	Nucleotide excision repair
MW	39.5 kDa
Gene Summary	The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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