Estrogen induced gene 121 protein (KIAA1324) (NM_001267049) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIG121; RP11-352P4.1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330749 representing NM_001267049. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTACAAATGGGCCAAGCCGAAAATCTGTAGCGAGGACCTTGAGGGGGCAGTGAAGCTGCCTGCCTCT GGTGTGAAGACCCACTGCCCACCCTGCAACCCAGGCTTCTTCAAAACCAACAACAGCACCTGCCAGCCC TGCCCATATGGTTCCTACTCCAATGGCTCAGACTGTACCCGCTGCCCTGCAGGGACTGAACCTGCTGTG GGATTTGAATACAAATGGTGGAACACGCTGCCCACAAACATGGAAACGACCGTTCTCAGTGGGATCAAC TTCGAGTACAAGGGCATGACAGGCTGGGAGGTGGCTGGTGATCACATTTACACAGCTGCTGGAGCCTCA GACAATGACTTCATGATTCTCACTCTGGTTGTGCCAGGATTTAGACCTCCGCAGTCGGTGATGGCAGAC ACAGAGAATAAAGAGGTGGCCAGAATCACATTTGTCTTTGAGACCCTCTGTTCTGTGAACTGTGAGCTC TACTTCATGGTGGGTGTGAATTCTAGGACCAACACTCCTGTGGAGACGTGGAAAGGTTCCAAAGGCAAA CAGTCCTATACCTACATCATTGAGGAGAACACTACCACGAGCTTCACCTGGGCCTTCCAGAGGACCACT TTTCATGAGGCAAGCAGGAAGTACACCAATGACGTTGCCAAGATCTACTCCATCAATGTCACCAATGTT ATGAATGGTGTGGCCTCCTACTGCCGTCCCTGTGCCCTAGAAGCCTCTGATGTGGGCTCCTCCTGCACC TCTTGTCCTGCTGGTTACTATATTGACCGAGATTCAGGAACCTGCCACTCCTGCCCCACTAACACAATT CTGAAAGCCCACCAGCCTTATGGTGTCCAGGCCTGTGTGCCCTGTGGTCCAGGGACCAAGAACAACAAG ATCCACTCTCTGTGCTACAACGATTGCACCTTCTCACGCAACACTCCGACCAGGACTTTCAACTACAAC TTCTCCGCTTTGGCAAACACTGTCACTCTTGCTGGAGGGCCAAGCTTCACTTCCAAAGGGCTGAAATAC TTCCATCACTTTACCCTCAGTCTCTGTGGAAACCAGGGTAGGAAAATGTCTGTGTGCACCGACAATGTC ACTGACCTCCGGATTCCTGAGGGTGAGTCAGGGTTCTCCAAATCTATCACAGCCTACGTCTGCCAGGCA GTCATCATCCCCCCAGAGGTGACAGGCTACAAGGCCGGGGTTTCCTCACAGCCTGTCAGCCTTGCTGAT CGACTTATTGGGGTGACAACAGATATGACTCTGGATGGAATCACCTCCCCAGCTGAACTTTTCCACCTG GAGTCCTTGGGAATACCGGACGTGATCTTCTTTTATAGGTCCAATGATGTGACCCAGTCCTGCAGTTCT GGGAGATCAACCACCATCCGCGTCAGGTGCAGTCCACAGAAAACTGTCCCTGGAAGTTTGCTGCTGCCA GGAACGTGCTCGGATGGGACCTGTGATGGCTGCAACTTCCACTTCCTGTGGGAGAGCGCGGCTGCTTGC CCGCTCTGCTCAGTGGCTGACTACCATGCTATCGTCAGCAGCTGTGTGGCTGGGATCCAGAAGACTACT TACGTGTGGCGAGAACCCAAGCTATGCTCTGGTGGCATTTCTCTGCCTGAGCAGAGAGTCACCATCTGC AAAACCATAGATTTCTGGCTGAAAGTGGGCATCTCTGCAGGCACCTGTACTGCCATCCTGCTCACCGTC TTGACCTGCTACTTTTGGAAAAAGAATCAAAAACTAGAGTACAAGTACTCCAAGCTGGTGATGAATGCT ACTCTCAAGGACTGTGACCTGCCAGCAGCTGACAGCTGCGCCATCATGGAAGGCGAGGATGTAGAGGAC GACCTCATCTTTACCAGCAAGAAGTCACTCTTTGGGAAGATCAAATCATTTACCTCCAAGAGGACTCCT GATGGATTTGACTCAGTGCCGCTGAAGACATCCTCAGGAGGCCTAGACATGGACCTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001267049
Insert Size	1992 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267049.1
RefSeq Size	5990 bp
RefSeq ORF	1992 bp
Locus ID	57535
Protein Families	Transmembrane
MW	72.3 kDa
Gene Summary	Expression of this gene is induced by estrogen and the encoded protein has been characterized as a transmembrane protein. The encoded protein has been found in to correlate with survival in certain carcinomas (PMID: 21102415) and may be important for cellular response to stress (PMID: 21072319). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream AUG compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1.

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