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Estrogen induced gene 121 protein (KIAA1324) (NM_001267049) Human Tagged ORF Clone
CAT＃: RG233063
- TrueORF®
KIAA1324 (tGFP-tagged) - Homo sapiens KIAA1324 (KIAA1324), transcript variant 3
ORF Plasmid: DDK
"NM_001267049" in other vectors (2)
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CNY 7,320.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EIG121; RP11-352P4.1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233063 representing NM_001267049
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAAATGGGCCAAGCCGAAAATCTGTAGCGAGGACCTTGAGGGGGCAGTGAAGCTGCCTGCCTCTG GTGTGAAGACCCACTGCCCACCCTGCAACCCAGGCTTCTTCAAAACCAACAACAGCACCTGCCAGCCCTG CCCATATGGTTCCTACTCCAATGGCTCAGACTGTACCCGCTGCCCTGCAGGGACTGAACCTGCTGTGGGA TTTGAATACAAATGGTGGAACACGCTGCCCACAAACATGGAAACGACCGTTCTCAGTGGGATCAACTTCG AGTACAAGGGCATGACAGGCTGGGAGGTGGCTGGTGATCACATTTACACAGCTGCTGGAGCCTCAGACAA TGACTTCATGATTCTCACTCTGGTTGTGCCAGGATTTAGACCTCCGCAGTCGGTGATGGCAGACACAGAG AATAAAGAGGTGGCCAGAATCACATTTGTCTTTGAGACCCTCTGTTCTGTGAACTGTGAGCTCTACTTCA TGGTGGGTGTGAATTCTAGGACCAACACTCCTGTGGAGACGTGGAAAGGTTCCAAAGGCAAACAGTCCTA TACCTACATCATTGAGGAGAACACTACCACGAGCTTCACCTGGGCCTTCCAGAGGACCACTTTTCATGAG GCAAGCAGGAAGTACACCAATGACGTTGCCAAGATCTACTCCATCAATGTCACCAATGTTATGAATGGTG TGGCCTCCTACTGCCGTCCCTGTGCCCTAGAAGCCTCTGATGTGGGCTCCTCCTGCACCTCTTGTCCTGC TGGTTACTATATTGACCGAGATTCAGGAACCTGCCACTCCTGCCCCACTAACACAATTCTGAAAGCCCAC CAGCCTTATGGTGTCCAGGCCTGTGTGCCCTGTGGTCCAGGGACCAAGAACAACAAGATCCACTCTCTGT GCTACAACGATTGCACCTTCTCACGCAACACTCCGACCAGGACTTTCAACTACAACTTCTCCGCTTTGGC AAACACTGTCACTCTTGCTGGAGGGCCAAGCTTCACTTCCAAAGGGCTGAAATACTTCCATCACTTTACC CTCAGTCTCTGTGGAAACCAGGGTAGGAAAATGTCTGTGTGCACCGACAATGTCACTGACCTCCGGATTC CTGAGGGTGAGTCAGGGTTCTCCAAATCTATCACAGCCTACGTCTGCCAGGCAGTCATCATCCCCCCAGA GGTGACAGGCTACAAGGCCGGGGTTTCCTCACAGCCTGTCAGCCTTGCTGATCGACTTATTGGGGTGACA ACAGATATGACTCTGGATGGAATCACCTCCCCAGCTGAACTTTTCCACCTGGAGTCCTTGGGAATACCGG ACGTGATCTTCTTTTATAGGTCCAATGATGTGACCCAGTCCTGCAGTTCTGGGAGATCAACCACCATCCG CGTCAGGTGCAGTCCACAGAAAACTGTCCCTGGAAGTTTGCTGCTGCCAGGAACGTGCTCGGATGGGACC TGTGATGGCTGCAACTTCCACTTCCTGTGGGAGAGCGCGGCTGCTTGCCCGCTCTGCTCAGTGGCTGACT ACCATGCTATCGTCAGCAGCTGTGTGGCTGGGATCCAGAAGACTACTTACGTGTGGCGAGAACCCAAGCT ATGCTCTGGTGGCATTTCTCTGCCTGAGCAGAGAGTCACCATCTGCAAAACCATAGATTTCTGGCTGAAA GTGGGCATCTCTGCAGGCACCTGTACTGCCATCCTGCTCACCGTCTTGACCTGCTACTTTTGGAAAAAGA ATCAAAAACTAGAGTACAAGTACTCCAAGCTGGTGATGAATGCTACTCTCAAGGACTGTGACCTGCCAGC AGCTGACAGCTGCGCCATCATGGAAGGCGAGGATGTAGAGGACGACCTCATCTTTACCAGCAAGAAGTCA CTCTTTGGGAAGATCAAATCATTTACCTCCAAGAGGACTCCTGATGGATTTGACTCAGTGCCGCTGAAGA CATCCTCAGGAGGCCTAGACATGGACCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233063 representing NM_001267049
Red=Cloning site Green=Tags(s) MYKWAKPKICSEDLEGAVKLPASGVKTHCPPCNPGFFKTNNSTCQPCPYGSYSNGSDCTRCPAGTEPAVG FEYKWWNTLPTNMETTVLSGINFEYKGMTGWEVAGDHIYTAAGASDNDFMILTLVVPGFRPPQSVMADTE NKEVARITFVFETLCSVNCELYFMVGVNSRTNTPVETWKGSKGKQSYTYIIEENTTTSFTWAFQRTTFHE ASRKYTNDVAKIYSINVTNVMNGVASYCRPCALEASDVGSSCTSCPAGYYIDRDSGTCHSCPTNTILKAH QPYGVQACVPCGPGTKNNKIHSLCYNDCTFSRNTPTRTFNYNFSALANTVTLAGGPSFTSKGLKYFHHFT LSLCGNQGRKMSVCTDNVTDLRIPEGESGFSKSITAYVCQAVIIPPEVTGYKAGVSSQPVSLADRLIGVT TDMTLDGITSPAELFHLESLGIPDVIFFYRSNDVTQSCSSGRSTTIRVRCSPQKTVPGSLLLPGTCSDGT CDGCNFHFLWESAAACPLCSVADYHAIVSSCVAGIQKTTYVWREPKLCSGGISLPEQRVTICKTIDFWLK VGISAGTCTAILLTVLTCYFWKKNQKLEYKYSKLVMNATLKDCDLPAADSCAIMEGEDVEDDLIFTSKKS LFGKIKSFTSKRTPDGFDSVPLKTSSGGLDMDL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001267049 |
| ORF Size | 1989 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001267049.1, NP_001253978.1 |
| RefSeq Size | 5990 bp |
| RefSeq ORF | 1991 bp |
| Locus ID | 57535 |
| Protein Families | Transmembrane |
| Gene Summary | Expression of this gene is induced by estrogen and the encoded protein has been characterized as a transmembrane protein. The encoded protein has been found in to correlate with survival in certain carcinomas (PMID: 21102415) and may be important for cellular response to stress (PMID: 21072319). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012] |
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