KCTD1 (NM_001258222) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C18orf5
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330647 representing NM_001258222. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTTCAGGACAGTCGGCCCAATATGTCAAGACCTCTGATCACTAGATCCCCTGCATCTCCACTGAAC AACCAAGGCATCCCTACTCCAGCACAACTCACAAAATCCAATGCGCCTGTCCACATTGATGTGGGCGGC CACATGTACACCAGCAGCCTGGCCACCCTCACCAAATACCCTGAATCCAGAATCGGAAGACTTTTTGAT GGTACAGAGCCCATTGTTTTGGACAGTCTCAAACAGCACTATTTCATTGACAGAGATGGACAGATGTTC AGATATATCTTGAATTTTCTACGAACATCCAAACTCCTCATTCCTGATGATTTCAAGGACTACACTTTG TTATATGAAGAGGCAAAATATTTTCAGCTTCAGCCCATGTTGTTGGAGATGGAAAGATGGAAGCAGGAC AGAGAAACTGGTCGATTTTCAAGGCCCTGTGAGTGCCTCGTCGTGCGTGTGGCCCCAGACCTCGGAGAA AGGATCACGCTAAGCGGTGACAAATCCTTGATAGAAGAAGTATTTCCAGAGATCGGCGACGTGATGTGT AACTCTGTCAATGCAGGCTGGAATCACGACTCGACGCACGTCATCAGGTTTCCACTAAATGGCTACTGT CACCTCAACTCAGTCCAGGTCCTCGAGAGGTTGCAGCAAAGAGGATTTGAAATCGTGGGCTCCTGTGGG GGAGGAGTAGACTCGTCCCAGTTCAGCGAATACGTCCTTCGGCGGGAACTGAGGCGGACGCCCCGTGTA CCCTCCGTCATCCGGATAAAGCAAGAGCCTCTGGACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258222
Insert Size	798 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258222.1
RefSeq Size	1671 bp
RefSeq ORF	798 bp
Locus ID	284252
Protein Families	Ion Channels: Other
MW	30.4 kDa
Gene Summary	This gene encodes a protein containing a BTB (Broad-complex, tramtrack and bric a brac), also known as a POZ (POxvirus and zinc finger) protein-protein interaction domain. The encoded protein negatively regulates the AP-2 family of transcription factors and the Wnt signaling pathway. A mechanism for the modulation of Wnt signaling has been proposed in which the encoded protein enhances ubiquitination and degradation of the beta-catenin protein. Mutations in this gene have been identified in Scalp-ear-nipple (SEN) syndrome. [provided by RefSeq, May 2017] Transcript Variant: This variant (5) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 3. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform b.

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