Transketolase (TKT) (NM_001258028) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HEL-S-48; HEL107; SDDHD; TK; TKT1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330630 representing NM_001258028. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGAGCTACCACAAGCCTGACCAGCAGAAGCTGCAGGCCTTGAAGGACACGGCCAACCGCCTACGT ATCAGCTCCATCCAGGCCACCACTGCGGCGGGCTCTGGCCACCCCACGTCATGCTGCAGCGCCGCAGAG ATCATGGCTGTCCTCTTTTTCCACACCATGCGCTACAAGTCCCAGGACCCCCGGAATCCGCACAATGAC CGCTTTGTGCTCTCCAAGGGCCATGCAGCTCCCATCCTCTACGCGGTCTGGGCTGAAGCTGGTTTCCTG GCCGAGGCGGAGCTGCTGAACCTGAGGAAGATCAGCTCCGACTTGGACGGGCACCCGGTCCCGAAACAA GCTTTCACCGACGTGGCCACTGGCTCCCTGGGCCAGGGCCTCGGGGCCGCTTGTGGGATGGCCTACACC GGCAAATACTTCGACAAGGCCAGCCTCCCGAGTAGCTGGGACTACAGCTACCGAGTCTATTGCTTGCTG GGAGACGGGGAGCTGTCAGAGGGCTCTGTATGGGAGGCCATGGCCTTCGCCAGCATCTATAAGCTGGAC AACCTTGTGGCCATTCTAGACATCAATCGCCTGGGCCAGAGTGACCCGGCCCCACTGCAGCACCAGATG GACATCTACCAGAAGCGGTGCGAGGCCTTCGGTTGGCATGCCATCATCGTGGATGGACACAGCGTGGAG GAGCTGTGCAAGGCCTTTGGCCAGGCCAAGCACCAGCCAACAGCCATCATTGCCAAGACCTTCAAGGGC CGAGGGATCACGGGGGTAGAAGATAAGGAGTCTTGGCATGGGAAGCCCCTCCCCAAAAACATGGCTGAG CAGATCATCCAGGAGATCTACAGCCAGATCCAGAGCAAAAAGAAGATCCTGGCAACCCCTCCACAGGAG GACGCACCCTCAGTGGACATTGCCAACATCCGCATGCCCAGCCTGCCCAGCTACAAAGTTGGGGACAAG ATAGCCACCCGCAAGGCCTACGGGCAGGCACTGGCCAAGCTGGGCCATGCCAGTGACCGCATCATCGCC CTGGATGGGGACACCAAAAATTCCACCTTCTCGGAGATCTTCAAAAAGGAGCACCCGGACCGCTTCATC GAGTGCTACATTGCTGAGCAGAACATGGTGAGCATCGCGGTGGGCTGTGCCACCCGCAACAGGACGGTG CCCTTCTGCAGCACTTTTGCAGCCTTCTTCACGCGGGCCTTTGACCAGATTCGCATGGCCGCCATCTCC GAGAGCAACATCAACCTCTGCGGCTCCCACTGCGGCGTTTCCATCGGGGAAGACGGGCCCTCCCAGATG GCCCTAGAAGATCTGGCTATGTTTCGGTCAGTCCCCACATCAACTGTCTTTTACCCAAGTGATGGCGTT GCTACAGAGAAGGCAGTGGAACTAGCCGCCAATACAAAGGGTATCTGCTTCATCCGGACCAGCCGCCCA GAAAATGCCATCATCTATAACAACAATGAGGACTTCCAGGTCGGACAAGCCAAGGTGGTCCTGAAGAGC AAGGATGACCAGGTGACCGTTATCGGGGCTGGGGTGACCCTGCACGAGGCCTTGGCCGCTGCCGAACTG CTGAAGAAAGAAAAGATCAACATCCGCGTGCTGGACCCCTTCACCATCAAGCCCCTGGACAGAAAACTC ATTCTCGACAGCGCTCGTGCCACCAAGGGCAGGATCCTCACCGTGGAGGACCATTATTATGAAGGTGGC ATTGGTGAGGCTGTGTCCAGTGCAGTAGTGGGCGAGCCTGGCATCACTGTCACCCACCTGGCAGTTAAC CGGGTACCAAGAAGTGGGAAGCCGGCTGAGCTGCTGAAGATGTTTGGTATCGACAGGGATGCCATTGCA CAAGCTGTGAGGGGCCTCATCACCAAGGCCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001258028
Insert Size	1896 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258028.1
RefSeq Size	2203 bp
RefSeq ORF	1896 bp
Locus ID	7086
UniProt ID	P29401
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Pentose phosphate pathway
MW	68.8 kDa
Gene Summary	This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (3) includes an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is longer than isoform 1.

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