VPS50 (NM_001257998) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCDC132; VPS54L
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330627 representing NM_001257998. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTAACTTTGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGACCCTCAAGCTGAACAAGAGCTT ATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATTGTTAAATATGAGCTGGAGAAG CTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATAGAGACAAATTGAAACAACAGCAAGCTGCA GTATCTAAAAAAGTGGCAGATTTAATCCTTGAAAAACAGCCTGCTTATGTAAAGGAACTTGAAAGAGTT ACCTCATTGCAGACAGGTCTTCAATTAGCTGCTGTTATCTGTACAAATGGGAGAAGACACTTGAATATT GCAAAGGAAGGTTTTACTCAAGCTAGTTTAGGCCTTCTTGCAAATCAAAGGAAACGTCAGTTGCTGATT GGACTTCTGAAATCTCTGAGAACTATAAAAACATTGCAAAGAACAGATGTACGGTTAAGTGAAATGCTG GAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCTGCCAGCACTTTTAAA CATTACAGTTGTATAAGTGAACTGAATTCAAAGCTGCAAGATACTTTGGAACAGATTGAGGAACAGCTG GACGTAGCTCTTTCCAAAATCTGCAAGAATTTTGACATTAACCATTATACCAAGGTTCAACAAGCTTAT CGACTTCTTGGAAAAACACAGACAGCAATGGATCAACTTCATATGCACTTCACCCAAGCCATTCACAAC ACCGTGTTTCAAGTTGTTCTTGGTTATGTGGAACTATGTGCAGGAAACACAGACACAAAATTCCAAAAG CTGCAATATAAGGATCTCTGTACACATGTTACACCAGACAGCTATATTCCATGCCTTGCAGACCTGTGC AAAGCACTATGGGAAGTTATGCTCAGCTATTATAGGACTATGGAATGGCATGAAAAGCATGACAATGAG GATACTGCTTCAGCTTCTGAAGGGAGTAATATGATAGGTACTGAAGAAACTAATTTTGATCGTGGCTAC ATAAAAAAGAAATTAGAACATGGACTTACACGAATATGGCAGGATGTTCAGCTAAAAGTAAAAACCTAC TTGCTTGGAACTGATTTGTCTATATTCAAATATGATGATTTCATCTTTGTTTTGGATATAATCAGCAGG TTGATGCAAGTTGGAGAAGAATTTTGTGGTAGCAAGTCTGAAGTTTTACAGGAATCTATTAGAAAACAA AGTGTCAATTATTTCAAGAATTACCATAGAACACGGCTCGATGAACTGAGAATGTTCTTAGAGAATGAG ACTTGGGAACTTTGTCCTGTTAAGTCAAATTTCAGCATCTTGCAACTTCATGAATTTAAATTCATGGAA CAGTCTCGCTCCCCATCAGTTTCACCTAGTAAACAGCCAGTCTCAACTTCTTCAAAAACAGTGACCTTG TTTGAGCAGTACTGTAGTGGTGGGAATCCATTTGAAATTCAGGCCAACCACAAAGATGAAGAAACAGAA GATGTCTTAGCTTCTAATGGGTATGAATCTGATGAACAAGAAAAGAGTGCCTATCAAGAGTATGACAGT GACAGTGATGTTCCTGAGGAACTCAAACGAGACTATGTGGATGAGCAGACAGGAGATGGTCCTGTGAAA AGTGTTTCTCGGGAAACTCTAAAAAGCAGGAAGAAATCAGATTACAGTCTAAATAAAGTGAATGCACCT ATCTTAACAAATACAACATTGAACGTCATAAGACTTGTTGGAAAATATATGCAGATGATGAACATTCTT AAGCCAATTGCCTTTGATGTTATTCATTTCATGTCTCAACTATTTGATTATTACTTGTATGCAATATAT ACCTTTTTTGGTCGGAATGATTCATTGGAATCAACTGGACTCGGCCTTAGTAGTAGTAGACTAAGAACA ACTCTAAACAGAATACAAGAAAGCCTTATTGATCTAGAAGTTTCAGCTGATCCTACTGCCACACTCACA GCAGCAGAAGAAAGAAAGGAGAAGGTGCCAAGTCCACACCTCAGTCACCTAGTGGTTTTGACATCTGGG GATACGCTGTATGGGTTGGCAGAAAGAGTGGTAGCCACGGAATCCTTGGTATTCTTGGCTGAACAGTTT GAGTTCCTTCAGCCACATCTGGATGCTGTGATGCCTGCAGTCAAAAAGCCCTTTCTTCAGCAGTTCTAT TCTCAGACAGTCTCAACCGCCAGTGAACTACGGAAACCAATTTACTGGATTGTAGCTGGTAAAGCCCTT GATTATGAACAGATGCTGCTTCTCATGGCTAATGTGAAATGGGATGTAAAAGAAATTATGTCACAGCAC AACATATATGTAGATGCACTATTAAAGGAATTTGAGCAGTTTAACAGGAGGCTAAATGAAGTTTCTAAG AGAGTTCGCATACCCTTGCCTGTGTCTAATATACTTTGGGAACATTGTATACGATTGGCTAATCGAACT ATTGTAGAAGGATATGCCAATGTCAAGAAATGCAGTAATGAGGGTCGTGCCCTGATGCAATTGGATTTT CAACAGTTTTTAATGAAACTTGAAAAACTAACAGATATTAGACCCATTCCTGATAAAGAATTTGTAGAA ACTTATATTAAAGCTTATTACCTAACTGAGAATGACATGGAACGGTGGATCAAAGAGCACAGGGAATAT TCAACGAAGCAGCTGACCAATCTGGTGAATGTTTGCCTGGGATCCCATATCAATAAGAAAGCAAGACAA AAACTTCTAGCAGCTATAGATGATATAGACAGACCTAAAAGATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257998
Insert Size	2805 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257998.1
RefSeq Size	5741 bp
RefSeq ORF	2805 bp
Locus ID	55610
UniProt ID	Q96JG6
MW	107.9 kDa
Gene Summary	Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate exon compared to variant 1. The resulting isoform (c) has a shorter and distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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