Beta TRCP (BTRC) (NM_001256856) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330538 representing NM_001256856. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGACCCGGCCGAGGCGGTGCTGCAAGAGAAGGCACTCAAGTTTATGTGCTCTATGCCCAGGTCTCTG TGGCTGGGCTGCTCCAGCCTGGCGGACAGCATGCCTTCGCTGCGATGCCTGTATAACCCAGGGACTGGC GCACTCACAGCTTTCCAGACATACAACAGCTGTGCCAGACTCTGCTTAAACCAAGAAACAGTATGTTTA GCAAGCACTGCTATGAAGACTGAGAATTGTGTGGCCAAAACAAAACTTGCCAATGGCACTTCCAGTATG ATTGTGCCCAAGCAACGGAAACTCTCAGCAAGCTATGAAAAGGAAAAGGAACTGTGTGTCAAATACTTT GAGCAGTGGTCAGAGTCAGATCAAGTGGAATTTGTGGAACATCTTATATCCCAAATGTGTCATTACCAA CATGGGCACATAAACTCGTATCTTAAACCTATGTTGCAGAGAGATTTCATAACTGCTCTGCCAGCTCGG GGATTGGATCATATTGCTGAGAACATTCTGTCATACCTGGATGCCAAATCACTATGTGCTGCTGAACTT GTGTGCAAGGAATGGTACCGAGTGACCTCTGATGGCATGCTGTGGAAGAAGCTTATCGAGAGAATGGTC AGGACAGATTCTCTGTGGAGAGGCCTGGCAGAACGAAGAGGATGGGGACAGTATTTATTCAAAAACAAA CCTCCTGACGGGAATGCTCCTCCCAACTCTTTTTATAGAGCACTTTATCCTAAAATTATACAAGACATT GAGACAATAGAATCTAATTGGAGATGTGGAAGACATAGTTTACAGAGAATTCACTGCCGAAGTGAAACA AGCAAAGGAGTTTACTGTTTACAGTATGATGATCAGAAAATAGTAAGCGGCCTTCGAGACAACACAATC AAGATCTGGGATAAAAACACATTGGAATGCAAGCGAATTCTCACAGGCCATACAGGTTCAGTCCTCTGT CTCCAGTATGATGAGAGAGTGATCATAACAGGATCATCGGATTCCACGGTCAGAGTGTGGGATGTAAAT ACAGGTGAAATGCTAAACACGTTGATTCACCATTGTGAAGCAGTTCTGCACTTGCGTTTCAATAATGGC ATGATGGTGACCTGCTCCAAAGATCGTTCCATTGCTGTATGGGATATGGCCTCCCCAACTGACATTACC CTCCGGAGGGTGCTGGTCGGACACCGAGCTGCTGTCAATGTTGTAGACTTTGATGACAAGTACATTGTT TCTGCATCTGGGGATAGAACTATAAAGGTATGGAACACAAGTACTTGTGAATTTGTAAGGACCTTAAAT GGACACAAACGAGGCATTGCCTGTTTGCAGTACAGGGACAGGCTGGTAGTGAGTGGCTCATCTGACAAC ACTATCAGATTATGGGACATAGAATGTGGTGCATGTTTACGAGTGTTAGAAGGCCATGAGGAATTGGTG CGTTGTATTCGATTTGATAACAAGAGGATAGTCAGTGGGGCCTATGATGGAAAAATTAAAGTGTGGGAT CTTGTGGCTGCTTTGGACCCCCGTGCTCCTGCAGGGACACTCTGTCTACGGACCCTTGTGGAGCATTCC GGAAGAGTTTTTCGACTACAGTTTGATGAATTCCAGATTGTCAGTAGTTCACATGATGACACAATCCTC ATCTGGGACTTCCTAAATGATCCAGCTGCCCAAGCTGAACCCCCCCGTTCCCCTTCTCGAACATACACC TACATCTCCAGATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256856
Insert Size	1740 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256856.1
RefSeq Size	6102 bp
RefSeq ORF	1740 bp
Locus ID	8945
Protein Families	Druggable Genome
Protein Pathways	Hedgehog signaling pathway, Oocyte meiosis, Ubiquitin mediated proteolysis, Wnt signaling pathway
MW	65.9 kDa
Gene Summary	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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