EPHX2 (NM_001256484) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ABHD20; CEH; SEH
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330440 representing NM_001256484. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAAGGAGAGATCACACTTTCCCAGTGGATACCACTCATGGAAGAAAACTGCAGGAAGTGCTCCGAG ACCGCTAAAGTCTGCCTCCCCAAGAATTTCTCCATAAAAGAAATCTTTGACAAGGCGATTTCAGCCAGA AAGATCAACCGCCCCATGCTCCAGGCAGCTCTCATGCTCAGGAAGAAAGGATTCACTACTGCCATCCTC ACCAACACCTGGCTGGACGACCGTGCTGAGAGAGATGGCCTGGCCCAGCTGATGTGTGAGCTGAAGATG CACTTTGACTTCCTGATAGAGTCGTGTCAGGTGGGAATGGTCAAACCTGAACCTCAGATCTACAAGTTT CTGCTGGACACCCTGAAGGCCAGCCCCAGTGAGGTCGTTTTTTTGGATGACATCGGGGCTAATCTGAAG CCAGCCCGTGACTTGGGAATGGTCACCATCCTGGTCCAGGACACTGACACGGCCCTGAAAGAACTGGAG AAAGTGACCGGAATCCAGCTTCTCAATACCCCGGCCCCTCTGCCGACCTCTTGCAATCCAAGTGACATG AGCCATGGGTACGTGACAGTAAAGCCCAGGGTCCGTCTGCATTTTGTGGAGCTGGGCTCCGGCCCTGCT GTGTGCCTCTGCCATGGATTTCCCGAGAGTTGGTATTCTTGGAGGTACCAGATCCCTGCTCTGGCCCAG GCAGGTTACCGGGTCCTAGCTATGGACATGAAAGGCTATGGAGAGTCATCTGCTCCTCCCGAAATAGAA GAATATTGCATGGAAGTGTTATGTAAGGAGATGGTAACCTTCCTGGATAAACTGGGCCTCTCTCAAGCA GTGTTCATTGGCCATGACTGGGGTGGCATGCTGGTGTGGTACATGGCTCTCTTCTACCCCGAGAGAGTG AGGGCGGTGGCCAGTTTGAATACTCCCTTCATACCAGCAAATCCCAACATGTCCCCTTTGGAGAGTATC AAAGCCAACCCAGTATTTGATTACCAGCTCTACTTCCAAGAACCAGGAGTGGCTGAGGCTGAACTGGAA CAGAACCTGAGTCGGACTTTCAAAAGCCTCTTCAGAGCAAGCGATGAGAGTGTTTTATCCATGCATAAA GTCTGTGAAGCGGGAGGACTTTTTGTAAATAGCCCAGAAGAGCCCAGCCTCAGCAGGATGGTCACTGAG GAGGAAATCCAGTTCTATGTGCAGCAGTTCAAGAAGTCTGGTTTCAGAGGTCCTCTAAACTGGTACCGA AACATGGAAAGGAACTGGAAGTGGGCTTGCAAAAGCTTGGGACGGAAGATCCTGATTCCGGCCCTGATG GTCACGGCGGAGAAGGACTTCGTGCTCGTTCCTCAGATGTCCCAGCACATGGAGGACTGGATTCCCCAC CTGAAAAGGGGACACATTGAGGACTGTGGGCACTGGACACAGATGGACAAGCCAACCGAGGTGAATCAG ATCCTCATTAAGTGGCTGGATTCTGATGCCCGGAACCCACCGGTGGTCTCAAAGATGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256484
Insert Size	1509 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256484.1
RefSeq Size	2122 bp
RefSeq ORF	1509 bp
Locus ID	2053
UniProt ID	P34913
Protein Pathways	Arachidonic acid metabolism, Metabolic pathways
MW	57.1 kDa
Gene Summary	This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (4) uses an alternate splice site at its 5' end and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Variants 2 and 4 encode the same isoform (b).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.